Restriction map for pF1KB8756
##################################
# NO CUT ENZYMES (  191 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvaI        AvaII       AviII       AvrII       BalI        
BamHI       BanI        BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BcnI        BfrI        
BglI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiWI       BsmAI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrFI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
Cac8I       CelII       CfoI        Cfr10I      Cfr13I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoO109I    EcoRI       EcoT22I     FseI        FspI        
HaeII       HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
HpaII       KasI        KpnI        Ksp632I     KspI        
MaeII       MamI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MslI        MspA1I      
MspI        MunI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NlaIV       NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshBI       Psp1406I    PvuI        PvuII       RcaI        
RsrII       SacII       SalI        SapI        Sau96I      
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TfiI        Tth111I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AluI          469  AGCT                 AG^CT
AspHI         468  GAGCTC               G[AT]GC[AT]^C
BanII         468  GAGCTC               G[GA]GC[CT]^C
BclI          554  TGATCA               T^GATCA
BfaI          356  CTAG                 C^TAG
BmyI          468  GAGCTC               G[AGT]GC[ACT]^C
BsiHKAI       468  GAGCTC               G[AT]GC[AT]^C
BsiYI         148  CCTACCAAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          148  CCTACCAAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI         379  GTCCC                GGGAC(10/14)
Bsp1286I      468  GAGCTC               G[AGT]GC[ACT]^C
BspMI          41  ACCTGC               ACCTGC(4/8)
BsrDI         454  GCAATG               GCAATG(2/0)
BssKI         482  CCTGG                ^CC[ACGT]GG
BstEII        180  GGTAACC              G^GT[ACGT]ACC
BstNI         482  CCTGG                CC^[AT]GG
BstPI         180  GGTAACC              G^GT[ACGT]ACC
DpnII         555  GATC                 ^GATC
EcoNI         148  CCTACCAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       180  GGTAACC              G^GT[ACGT]ACC
EcoRII        482  CCTGG                ^CC[AT]GG
EcoRV         360  GATATC               GAT^ATC
EcoT14I       185  CCTTGG               C^C[AT][AT]GG
FauI           91  GCGGG                CCCGC(4/6)
FbaI          554  TGATCA               T^GATCA
HphI          460  GGTGA                GGTGA(8/7)
MaeI          356  CTAG                 C^TAG
MboI          555  GATC                 ^GATC
MnlI          414  GAGG                 CCTC(7/6)
MvaI          482  CCTGG                CC^[AT]GG
NdeII         555  GATC                 ^GATC
NlaIII        547  CATG                 CATG^
PshAI          99  GACAACTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          118  CTGCAG               CTGCA^G
SacI          468  GAGCTC               GAGCT^C
Sau3AI        555  GATC                 ^GATC
ScrFI         482  CCTGG                CC^[ACGT]GG
SfaNI         272  GATGC                GCATC(5/9)
SfcI          118  CTGCAG               C^T[GA][CT]AG
SmlI          535  CTTGAG               C^T[CT][GA]AG
StyI          185  CCTTGG               C^C[AT][AT]GG
TaqI          314  TCGA                 T^CGA
TthHB8I       314  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AciI           91  GCGG                 CCGC(-3/-1)
              579  GCGG                
AcsI          130  GAATTT               [GA]^AATT[CT]
              192  AAATTC              
AfaI          145  GTAC                 GT^AC
              385  GTAC                
ApoI          130  GAATTT               [GA]^AATT[CT]
              192  AAATTC              
BbvI          492  GCAGC                GCAGC(8/12)
              117  GCTGC               
BsaJI         185  CCTTGG               C^C[ACGT][ACGT]GG
              481  CCCTGG              
BstF5I        222  GGATG                GGATG(2/0)
              320  GGATG               
DdeI          167  CTGAG                C^T[ACGT]AG
              466  CTGAG               
FokI          222  GGATG                GGATG(9/13)
              320  GGATG               
HaeIII        248  GGCC                 GG^CC
              520  GGCC                
HinfI         301  GACTC                G^A[ACGT]TC
              377  GAGTC               
MboII         325  GAAGA                GAAGA(8/7)
              566  GAAGA               
MwoI          275  GCACAGATGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              486  GCATTTGCAGC         
PleI          301  GACTC                GAGTC(4/5)
              377  GAGTC               
RsaI          145  GTAC                 GT^AC
              385  GTAC                
TseI          117  GCTGC                G^C[AT]GC
              492  GCAGC               
Tsp45I        205  GTCAC                ^GT[GC]AC
              426  GTCAC               
TspRI         165  CACTG                CA[GC]TG[ACGT][ACGT]^
              251  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (    6 enzymes) #
#########################################

Fnu4HI      ItaI        MaeIII      MseI        Tru9I       
Tsp509I     
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