Restriction map for pF1KB8755
##################################
# NO CUT ENZYMES (  168 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
Alw44I      AlwI        AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BbeI        
BbrPI       BcgI        BciVI       BfaI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstYI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
DsaI        EagI        EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT22I     FseI        FspI        
HaeII       HgaI        Hin1I       HincII      HindII      
HindIII     HpaI        KasI        KpnI        Ksp632I     
KspI        MaeI        MamI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MseI        
MunI        NaeI        NarI        NcoI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        TfiI        Tru9I       Tsp509I     TspRI       
Tth111I     Van91I      XbaI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   39 enzymes) #
###################################

AccII         385  CGCG                 CG^CG
AfaI          255  GTAC                 GT^AC
AhdI          516  GACCGTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI         516  GACCGTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII         515  GGACC                G^G[AT]CC
BbsI          121  GAAGAC               GAAGAC(2/6)
BbvCI         449  CCTCAGC              CCTCAGC(-5/-2)
BpuAI         121  GAAGAC               GAAGAC(2/6)
BsaI          445  GAGACC               GGTCTC(1/5)
BseRI         283  GAGGAG               GAGGAG(10/8)
BsgI          391  GTGCAG               GTGCAG(16/14)
BsmFI         326  GGGAC                GGGAC(10/14)
BsrDI         167  CATTGC               GCAATG(2/0)
BsrFI         131  ACCGGC               [GA]^CCGG[CT]
BsrI          198  CCAGT                ACTGG(1/-1)
BstUI         385  CGCG                 CG^CG
BstXI         344  CCATCATGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI          510  GCGC                 GCG^C
Cfr10I        131  ACCGGC               [GA]^CCGG[CT]
Eam1105I      516  GACCGTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI          419  CCCGC                CCCGC(4/6)
HhaI          510  GCGC                 GCG^C
HinP1I        510  GCGC                 G^CGC
HinfI         522  GAGTC                G^A[ACGT]TC
HphI          176  TCACC                GGTGA(8/7)
MboII         121  GAAGA                GAAGA(8/7)
MvnI          385  CGCG                 CG^CG
NdeI          159  CATATG               CA^TATG
NlaIV         191  GGAGCC               GG[ACGT]^[ACGT]CC
PleI          522  GAGTC                GAGTC(4/5)
PvuII         144  CAGCTG               CAG^CTG
RsaI          255  GTAC                 GT^AC
SfaNI         338  GCATC                GCATC(5/9)
SmlI          289  CTCAAG               C^T[CT][GA]AG
StuI          410  AGGCCT               AGG^CCT
TaqI          533  TCGA                 T^CGA
Tsp45I        149  GTGAC                ^GT[GC]AC
TthHB8I       533  TCGA                 T^CGA
XcmI          344  CCATCATGCTGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AlwNI         214  CAGCGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              487  CAGGAGCTG           
AspHI          60  GAGCTC               G[AT]GC[AT]^C
              286  GAGCTC              
BclI          140  TGATCA               T^GATCA
              590  TGATCA              
BpmI          526  CTCCAG               CTGGAG(16/14)
              502  CTGGAG              
BsiHKAI        60  GAGCTC               G[AT]GC[AT]^C
              286  GAGCTC              
BsmAI         445  GAGAC                GTCTC(1/5)
              524  GTCTC               
Cfr13I        417  GGCCC                G^G[ACGT]CC
              515  GGACC               
DdeI            5  CTGAG                C^T[ACGT]AG
              450  CTCAG               
EaeI           29  TGGCCG               [CT]^GGCC[GA]
              206  CGGCCA              
EcoT14I       278  CCTTGG               C^C[AT][AT]GG
              413  CCTTGG              
FbaI          140  TGATCA               T^GATCA
              590  TGATCA              
MaeII         329  ACGT                 A^CGT
              470  ACGT                
MaeIII         14  GTAAC                ^GT[ACGT]AC
              149  GTGAC               
MspA1I        144  CAGCTG               C[AC]G^C[GT]G
              214  CAGCGG              
MwoI           47  GCGGTGTTGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              377  GCCCCAGCCGC         
SacI           60  GAGCTC               GAGCT^C
              286  GAGCTC              
Sau96I        417  GGCCC                G^G[ACGT]CC
              515  GGACC               
StyI          278  CCTTGG               C^C[AT][AT]GG
              413  CCTTGG              


#########################################
# 3 OR MORE CUT ENZYMES (   33 enzymes) #
#########################################

AciI        AluI        BanII       BbvI        BcnI        
BmyI        BsaJI       BsiYI       BslI        Bsp1286I    
BssKI       BstF5I      BstNI       Cac8I       DpnII       
EcoRII      Fnu4HI      FokI        HaeIII      HapII       
HpaII       ItaI        MboI        MnlI        MslI        
MspI        MvaI        NciI        NdeII       NlaIII      
Sau3AI      ScrFI       TseI        
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