Restriction map for pF1KB8755
 (Linear) (Six-base) MAPSORT of: KIBB8755.seq  Check: 5716  from: 1  to: 597



KIBB8755.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:08  ..

AhdI GACnn_n'nnGTC

Cuts at:      0     521     597 
   Size:        521      76
 
AlwNI CAG_nnn'CTG

Cuts at:      0     219     492     597 
   Size:        219     273     105

  Fragments arranged by size: 

                 273     219     105
 
BanII G_rGCy'C

Cuts at:      0      64     290     379     597 
   Size:         64     226      89     218

  Fragments arranged by size: 

                 226     218      89      64
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     128     597 
   Size:        128     469
 
BbvCI CC'TCA_GC

Cuts at:      0     450     597 
   Size:        450     147
 
BclI T'GATC_A

Cuts at:      0     140     590     597 
   Size:        140     450       7

  Fragments arranged by size: 

                 450     140       7
 
Bme1580I G_kGCm'C

Cuts at:      0     239     597 
   Size:        239     358
 
BmgBI CAC'GTC

Cuts at:      0     330     597 
   Size:        330     267
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     511     523     597 
   Size:        511      12      74

  Fragments arranged by size: 

                 511      74      12
 
Bpu10I CC'TnA_GC

Cuts at:      0       5     450     597 
   Size:          5     445     147

  Fragments arranged by size: 

                 445     147       5
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     274     597 
   Size:        274     323
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     439     597 
   Size:        439     158
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     298     597 
   Size:        298     299
 
BseYI C'CCAG_C

Cuts at:      0     379     597 
   Size:        379     218
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     412     597 
   Size:        412     185
 
BsiHKAI G_wGCw'C

Cuts at:      0      64     290     597 
   Size:         64     226     307

  Fragments arranged by size: 

                 307     226      64
 
Bsp1286I G_dGCh'C

Cuts at:      0      64     239     290     379     597 
   Size:         64     175      51      89     218

  Fragments arranged by size: 

                 218     175      89      64      51
 
BsrDI GCAATG_nn'

Cuts at:      0     166     597 
   Size:        166     431
 
BsrFI r'CCGG_y

Cuts at:      0     131     597 
   Size:        131     466
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     351     597 
   Size:        351     246
 
EaeI y'GGCC_r

Cuts at:      0      29     206     597 
   Size:         29     177     391

  Fragments arranged by size: 

                 391     177      29
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     421     597 
   Size:        421     176
 
EcoICRI GAG'CTC

Cuts at:      0      62     288     597 
   Size:         62     226     309

  Fragments arranged by size: 

                 309     226      62
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     511     523     597 
   Size:        511      12      74

  Fragments arranged by size: 

                 511      74      12
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     453     597 
   Size:        453     144
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     507     539     597 
   Size:        507      32      58

  Fragments arranged by size: 

                 507      58      32
 
MslI CAynn'nnrTG

Cuts at:      0     250     352     429     597 
   Size:        250     102      77     168

  Fragments arranged by size: 

                 250     168     102      77
 
MspA1I CmG'CkG

Cuts at:      0     146     216     597 
   Size:        146      70     381

  Fragments arranged by size: 

                 381     146      70
 
NdeI CA'TA_TG

Cuts at:      0     160     597 
   Size:        160     437
 
PfoI T'CCnGG_A

Cuts at:      0      95     485     597 
   Size:         95     390     112

  Fragments arranged by size: 

                 390     112      95
 
PvuII CAG'CTG

Cuts at:      0     146     597 
   Size:        146     451
 
SacI G_AGCT'C

Cuts at:      0      64     290     597 
   Size:         64     226     307

  Fragments arranged by size: 

                 307     226      64
 
SmlI C'TyrA_G

Cuts at:      0     289     597 
   Size:        289     308
 
StuI AGG'CCT

Cuts at:      0     412     597 
   Size:        412     185
 
StyI C'CwwG_G

Cuts at:      0     278     413     597 
   Size:        278     135     184

  Fragments arranged by size: 

                 278     184     135
 
TatI w'GTAC_w

Cuts at:      0     254     597 
   Size:        254     343
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     351     383     597 
   Size:        351      32     214

  Fragments arranged by size: 

                 351     214      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     351     597 
   Size:        351     246
 

 Enzymes that do cut:

     AhdI    AlwNI    BanII     BbsI    BbvCI     BclI Bme1580I    BmgBI
     BpmI   Bpu10I    BpuEI     BsaI    BseRI    BseYI     BsgI  BsiHKAI
 Bsp1286I    BsrDI    BsrFI    BstXI     EaeI     EciI  EcoICRI  Eco57MI
     FalI    Hin4I     MslI   MspA1I     NdeI     PfoI    PvuII     SacI
     SmlI     StuI     StyI     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI     BmrI
     BmtI     BplI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     NaeI     NarI     NcoI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI    TaqII
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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