Restriction map for pF1KB8746
##################################
# NO CUT ENZYMES (  170 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AlwNI       AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AviII       AvrII       BamHI       BanI        
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BfaI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaI        BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmAI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrGI       
BsrI        BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstF5I      BstPI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DdeI        DpnII       DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FokI        FseI        FspI        
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MaeI        MamI        MboI        
MfeI        MflI        MluI        MroI        MseI        
MslI        MunI        NarI        NdeI        NdeII       
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        Sau3AI      
SbfI        ScaI        SexAI       SfaNI       SfoI        
SfuI        SgrAI       SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TfiI        Tru9I       Tsp509I     Tth111I     
Van91I      XbaI        XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   26 enzymes) #
###################################

AcyI          420  GGCGTC               G[GA]^CG[CT]C
AfaI           71  GTAC                 GT^AC
BalI          173  TGGCCA               TGG^CCA
BanII          14  GAGCCC               G[GA]GC[CT]^C
BpmI          298  CTGGAG               CTGGAG(16/14)
BsaHI         420  GGCGTC               G[GA]^CG[CT]C
BsmFI         449  GGGAC                GGGAC(10/14)
EarI          488  CTCTTC               CTCTTC(1/4)
EcoNI         321  CCTGCTGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII         456  GGCGCT               [GA]GCGC^[CT]
Hin1I         420  GGCGTC               G[GA]^CG[CT]C
HinfI         125  GACTC                G^A[ACGT]TC
HphI          278  TCACC                GGTGA(8/7)
Ksp632I       488  CTCTTC               CTCTTC(1/4)
MaeII         452  ACGT                 A^CGT
MboII         489  TCTTC                GAAGA(8/7)
MluNI         173  TGGCCA               TGG^CCA
MscI          173  TGGCCA               TGG^CCA
PleI          125  GACTC                GAGTC(4/5)
RsaI           71  GTAC                 GT^AC
SapI          487  GCTCTTC              GCTCTTC(1/4)
SfiI          364  GGCCCGCTGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
TaqI          239  TCGA                 T^CGA
TspRI         262  CACTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       239  TCGA                 T^CGA
XcmI          515  CCACAGAGGCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AccII          79  CGCG                 CG^CG
              474  CGCG                
AvaII         303  GGACC                G^G[AT]CC
              330  GGTCC               
BglI          142  GCCCTGCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              365  GCCCGCTGGGC         
BmyI           14  GAGCCC               G[AGT]GC[ACT]^C
              196  GTGCCC              
Bsp1286I       14  GAGCCC               G[AGT]GC[ACT]^C
              196  GTGCCC              
BsrFI         147  GCCGGC               [GA]^CCGG[CT]
              374  GCCGGC              
BstUI          79  CGCG                 CG^CG
              474  CGCG                
Cfr10I        147  GCCGGC               [GA]^CCGG[CT]
              374  GCCGGC              
DraII         317  AGGCCCT              [GA]G^G[ACGT]CC[CT]
              521  AGGCCCT             
EaeI          173  TGGCCA               [CT]^GGCC[GA]
              470  TGGCCG              
EcoO109I      317  AGGCCCT              [GA]G^G[ACGT]CC[CT]
              521  AGGCCCT             
HgaI          246  GACGC                GACGC(5/10)
              421  GCGTC               
MaeIII        244  GTGAC                ^GT[ACGT]AC
              266  GTGAC               
MvnI           79  CGCG                 CG^CG
              474  CGCG                
NaeI          147  GCCGGC               GCC^GGC
              374  GCCGGC              
NcoI           92  CCATGG               C^CATGG
              360  CCATGG              
NgoMIV        147  GCCGGC               G^CCGGC
              374  GCCGGC              
SmaI          201  CCCGGG               CCC^GGG
              435  CCCGGG              
Tsp45I        244  GTGAC                ^GT[GC]AC
              266  GTGAC               
XmaCI         201  CCCGGG               C^CCGGG
              435  CCCGGG              
XmaI          201  CCCGGG               C^CCGGG
              435  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   41 enzymes) #
#########################################

AciI        AluI        AvaI        BbvI        BcnI        
BsaJI       BsiYI       BslI        BsoBI       BspMI       
BssKI       BstNI       Cac8I       CfoI        Cfr13I      
DsaI        EcoRII      EcoT14I     FauI        Fnu4HI      
HaeIII      HapII       HhaI        HinP1I      HpaII       
ItaI        MnlI        MspA1I      MspI        MvaI        
MwoI        NciI        NlaIII      NlaIV       PstI        
PvuII       Sau96I      ScrFI       SfcI        StyI        
TseI        
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