Restriction map for pF1KB8745
 (Linear) (Six-base) MAPSORT of: KIBB8745.seq  Check: 3713  from: 1  to: 1206



KIBB8745.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:08  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     286    1206 
   Size:        286     920
 
AfeI AGC'GCT

Cuts at:      0     253    1206 
   Size:        253     953
 
AflII C'TTAA_G

Cuts at:      0     574    1206 
   Size:        574     632
 
AflIII A'CryG_T

Cuts at:      0      18    1206 
   Size:         18    1188
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1137    1206 
   Size:       1137      69
 
ApaI G_GGCC'C

Cuts at:      0      66     277    1206 
   Size:         66     211     929

  Fragments arranged by size: 

                 929     211      66
 
ApoI r'AATT_y

Cuts at:      0     397    1113    1206 
   Size:        397     716      93

  Fragments arranged by size: 

                 716     397      93
 
AvaI C'yCGr_G

Cuts at:      0     144    1206 
   Size:        144    1062
 
BanI G'GyrC_C

Cuts at:      0     156     871    1206 
   Size:        156     715     335

  Fragments arranged by size: 

                 715     335     156
 
BanII G_rGCy'C

Cuts at:      0      66     277     751     948    1206 
   Size:         66     211     474     197     258

  Fragments arranged by size: 

                 474     258     211     197      66
 
BbvCI CC'TCA_GC

Cuts at:      0      76    1206 
   Size:         76    1130
 
BclI T'GATC_A

Cuts at:      0    1118    1206 
   Size:       1118      88
 
BglI GCCn_nnn'nGGC

Cuts at:      0     118    1206 
   Size:        118    1088
 
Bme1580I G_kGCm'C

Cuts at:      0      66     277     876    1206 
   Size:         66     211     599     330

  Fragments arranged by size: 

                 599     330     211      66
 
BmtI G_CTAG'C

Cuts at:      0       8    1206 
   Size:          8    1198
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     450    1206 
   Size:        450     756
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     451     649    1114    1206 
   Size:        451     198     465      92

  Fragments arranged by size: 

                 465     451     198      92
 
Bpu10I CC'TnA_GC

Cuts at:      0      76    1206 
   Size:         76    1130
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     232     404    1206 
   Size:        232     172     802

  Fragments arranged by size: 

                 802     232     172
 
BsaAI yAC'GTr

Cuts at:      0      19    1206 
   Size:         19    1187
 
BsaBI GATnn'nnATC

Cuts at:      0     763    1206 
   Size:        763     443
 
BsaHI Gr'CG_yC

Cuts at:      0     209    1206 
   Size:        209     997
 
BsaWI w'CCGG_w

Cuts at:      0     512    1206 
   Size:        512     694
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     250    1206 
   Size:        250     956
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     481    1206 
   Size:        481     725
 
BsmI GAATG_Cn'

Cuts at:      0      48    1206 
   Size:         48    1158
 
Bsp1286I G_dGCh'C

Cuts at:      0      66     277     751     876     948    1206 
   Size:         66     211     474     125      72     258

  Fragments arranged by size: 

                 474     258     211     125      72      66
 
BsrBI CCG'CTC

Cuts at:      0     612    1206 
   Size:        612     594
 
BsrDI GCAATG_nn'

Cuts at:      0      11     789    1206 
   Size:         11     778     417

  Fragments arranged by size: 

                 778     417      11
 
BsrFI r'CCGG_y

Cuts at:      0      32    1206 
   Size:         32    1174
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     205     354     627    1206 
   Size:        205     149     273     579

  Fragments arranged by size: 

                 579     273     205     149
 
Bsu36I CC'TnA_GG

Cuts at:      0     268     902    1206 
   Size:        268     634     304

  Fragments arranged by size: 

                 634     304     268
 
BtgI C'CryG_G

Cuts at:      0     390    1206 
   Size:        390     816
 
BtsI GCAGTG_nn'

Cuts at:      0     384     777    1206 
   Size:        384     393     429

  Fragments arranged by size: 

                 429     393     384
 
EaeI y'GGCC_r

Cuts at:      0     110    1206 
   Size:        110    1096
 
EarI CTCTTCn'nnn_

Cuts at:      0     650    1206 
   Size:        650     556
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     286     451     649    1114    1206 
   Size:        286     165     198     465      92

  Fragments arranged by size: 

                 465     286     198     165      92
 
EcoO109I rG'GnC_Cy

Cuts at:      0      80     273     274     558     849    1206 
   Size:         80     193       1     284     291     357

  Fragments arranged by size: 

                 357     291     284     193      80       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     454    1206 
   Size:        454     752
 
HaeII r_GCGC'y

Cuts at:      0     255    1206 
   Size:        255     951
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1110    1142    1206 
   Size:       1110      32      64

  Fragments arranged by size: 

                1110      64      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     588    1206 
   Size:        588     618
 
MscI TGG'CCA

Cuts at:      0     112    1206 
   Size:        112    1094
 
MslI CAynn'nnrTG

Cuts at:      0     295    1206 
   Size:        295     911
 
MspA1I CmG'CkG

Cuts at:      0    1037    1206 
   Size:       1037     169
 
NaeI GCC'GGC

Cuts at:      0      34    1206 
   Size:         34    1172
 
NcoI C'CATG_G

Cuts at:      0     390    1206 
   Size:        390     816
 
NgoMIV G'CCGG_C

Cuts at:      0      32    1206 
   Size:         32    1174
 
NheI G'CTAG_C

Cuts at:      0       4    1206 
   Size:          4    1202
 
PasI CC'CwG_GG

Cuts at:      0      84    1206 
   Size:         84    1122
 
PfoI T'CCnGG_A

Cuts at:      0    1130    1206 
   Size:       1130      76
 
PmlI CAC'GTG

Cuts at:      0      19    1206 
   Size:         19    1187
 
PpuMI rG'GwC_Cy

Cuts at:      0      80     558     849    1206 
   Size:         80     478     291     357

  Fragments arranged by size: 

                 478     357     291      80
 
PshAI GACnn'nnGTC

Cuts at:      0     199    1206 
   Size:        199    1007
 
PspOMI G'GGCC_C

Cuts at:      0      62     273    1206 
   Size:         62     211     933

  Fragments arranged by size: 

                 933     211      62
 
SmlI C'TyrA_G

Cuts at:      0     247     419     574    1206 
   Size:        247     172     155     632

  Fragments arranged by size: 

                 632     247     172     155
 
StyI C'CwwG_G

Cuts at:      0      58     165     390    1206 
   Size:         58     107     225     816

  Fragments arranged by size: 

                 816     225     107      58
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      88    1206 
   Size:         88    1118
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     863    1206 
   Size:        863     343
 
XmnI GAAnn'nnTTC

Cuts at:      0     139    1206 
   Size:        139    1067
 

 Enzymes that do cut:

     AcuI     AfeI    AflII   AflIII    AlwNI     ApaI     ApoI     AvaI
     BanI    BanII    BbvCI     BclI     BglI Bme1580I     BmtI     BplI
     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI
     BsgI     BsmI Bsp1286I    BsrBI    BsrDI    BsrFI    BstXI   Bsu36I
     BtgI     BtsI     EaeI     EarI  Eco57MI EcoO109I     FalI    HaeII
    Hin4I     MmeI     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV
     NheI     PasI     PfoI     PmlI    PpuMI    PshAI   PspOMI     SmlI
     StyI    TaqII     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AgeI     AhdI     AleI
     AlfI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbeI     BbsI     BcgI    BciVI    BfrBI    BglII     BlpI
    BmgBI     BmrI     BsaI    BsaXI    BseYI    BsiEI  BsiHKAI    BsiWI
    BsmBI    BspEI    BspHI    BspMI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstYI  BstZ17I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     NarI     NdeI     NotI     NruI     NsiI     NspI
     PacI     PciI    PflMI     PmeI     PpiI     PsiI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com