Restriction map for pF1KB8744
##################################
# NO CUT ENZYMES (  175 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
Alw44I      AlwI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspI        AviII       AvrII       BalI        
BamHI       BanI        BbeI        BcgI        BciVI       
BclI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsiEI       BsiWI       
BsmFI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrFI       
BsrGI       BsrI        BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstF5I      BstPI       BstXI       
BstYI       BstZ17I     CelII       Cfr10I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        EarI        EclXI       
Eco47III    Eco52I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FokI        
FseI        FspI        Hin1I       HincII      HindII      
HindIII     HpaI        HphI        KasI        KpnI        
Ksp632I     KspI        MaeI        MaeIII      MamI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MseI        MspA1I      MunI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NlaIII      NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmeI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsrII       SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        TfiI        Tru9I       Tsp45I      Tsp509I     
TspRI       Tth111I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AfaI          147  GTAC                 GT^AC
AhdI          112  GACATCGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI          76  CAGTTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI           34  CCTGAGG              CC^T[ACGT]AGG
AspEI         112  GACATCGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII         155  GGACC                G^G[AT]CC
BbrPI         241  CACGTG               CAC^GTG
BbsI          375  GTCTTC               GAAGAC(2/6)
BbvCI         298  GCTGAGG              CCTCAGC(-5/-2)
BbvI          246  GCAGC                GCAGC(8/12)
BcnI           87  CCCGG                CC^[GC]GG
BpmI           52  CTGGAG               CTGGAG(16/14)
BpuAI         375  GTCTTC               GAAGAC(2/6)
BsaAI         241  CACGTG               [CT]AC^GT[GA]
BsgI          244  GTGCAG               GTGCAG(16/14)
BsiYI          81  CCTGCTCCCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           81  CCTGCTCCCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         120  GTCTC                GTCTC(1/5)
BsmBI         119  CGTCTC               CGTCTC(1/5)
BstAPI         75  GCAGTTCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I         34  CCTGAGG              CC^T[ACGT]AGG
DpnII         198  GATC                 ^GATC
Eam1105I      112  GACATCGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I         34  CCTGAGG              CC^T[ACGT]AGG
EcoT14I       311  CCAAGG               C^C[AT][AT]GG
FauI          141  GCGGG                CCCGC(4/6)
HapII          88  CCGG                 C^CGG
HgaI          118  GCGTC                GACGC(5/10)
HinfI         373  GAGTC                G^A[ACGT]TC
HpaII          88  CCGG                 C^CGG
MaeII         242  ACGT                 A^CGT
MboI          198  GATC                 ^GATC
MslI          250  CACCCGAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI           88  CCGG                 C^CGG
NciI           87  CCCGG                CC^[GC]GG
NdeII         198  GATC                 ^GATC
PleI          373  GAGTC                GAGTC(4/5)
PmaCI         241  CACGTG               CAC^GTG
PmlI          241  CACGTG               CAC^GTG
RsaI          147  GTAC                 GT^AC
SacI          346  GAGCTC               GAGCT^C
Sau3AI        198  GATC                 ^GATC
StuI          215  AGGCCT               AGG^CCT
StyI          311  CCAAGG               C^C[AT][AT]GG
TseI          246  GCAGC                G^C[AT]GC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AluI          167  AGCT                 AG^CT
              347  AGCT                
AspHI         133  GAGCAC               G[AT]GC[AT]^C
              346  GAGCTC              
AvaI           58  CCCGAG               C^[CT]CG[GA]G
              252  CCCGAG              
BanII          55  GAGCCC               G[GA]GC[CT]^C
              346  GAGCTC              
BsiHKAI       133  GAGCAC               G[AT]GC[AT]^C
              346  GAGCTC              
BsoBI          58  CCCGAG               C^[CT]CG[GA]G
              252  CCCGAG              
BspMI         149  ACCTGC               ACCTGC(4/8)
              207  GCAGGT              
BstNI           9  CCTGG                CC^[AT]GG
              158  CCAGG               
Cfr13I          5  GGCCC                G^G[ACGT]CC
              155  GGACC               
DdeI           35  CTGAG                C^T[ACGT]AG
              299  CTGAG               
EcoRII          9  CCTGG                ^CC[AT]GG
              158  CCAGG               
Fnu4HI          3  GCGGC                GC^[ACGT]GC
              246  GCAGC               
HaeII          30  AGCGCC               [GA]GCGC^[CT]
              229  AGCGCC              
HaeIII          5  GGCC                 GG^CC
              216  GGCC                
ItaI            3  GCGGC                GC^[ACGT]GC
              246  GCAGC               
MboII         324  GAAGA                GAAGA(8/7)
              376  TCTTC               
MvaI            9  CCTGG                CC^[AT]GG
              158  CCAGG               
NlaIV           5  GGCCCC               GG[ACGT]^[ACGT]CC
               54  GGAGCC              
Sau96I          5  GGCCC                G^G[ACGT]CC
              155  GGACC               
SfaNI         102  GATGC                GCATC(5/9)
              336  GATGC               
TaqI           71  TCGA                 T^CGA
              344  TCGA                
TthHB8I        71  TCGA                 T^CGA
              344  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   16 enzymes) #
#########################################

AccII       AciI        BmyI        BsaJI       BseRI       
Bsp1286I    BssKI       BstUI       Cac8I       CfoI        
HhaI        HinP1I      MnlI        MvnI        MwoI        
ScrFI       
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