Restriction map for pF1KB8734
##################################
# NO CUT ENZYMES (  138 enzymes) #
##################################

AatII       Acc65I      AccI        AcsI        AcyI        
AflIII      AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
Asp718I     AspEI       AspI        AvaI        AviII       
BalI        BanII       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BcnI        BglI        
BlpI        Bpu1102I    BsaAI       BsaHI       BsaI        
BsiEI       BsmBI       BsoBI       Bsp1407I    BspDI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstXI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        DrdI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HgaI        Hin1I       HincII      HindII      
HpaI        KasI        KpnI        KspI        MluI        
MluNI       MscI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PvuI        PvuII       RcaI        
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        TaqI        
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccIII        114  TCCGGA               T^CCGGA
AclI          999  AACGTT               AA^CGTT
AflII        1287  CTTAAG               C^TTAAG
AlwNI         812  CAGATTCTG            CAG[ACGT][ACGT][ACGT]^CTG
AseI          543  ATTAAT               AT^TAAT
AsnI          543  ATTAAT               AT^TAAT
Asp700I       169  GAAAGCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI        1026  GGATCC               G^GATCC
BanI           41  GGCACC               G^G[CT][GA]CC
BbsI          154  GTCTTC               GAAGAC(2/6)
BfrI         1287  CTTAAG               C^TTAAG
BglII        1173  AGATCT               A^GATCT
BpuAI         154  GTCTTC               GAAGAC(2/6)
BsaBI        1579  GATTTTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         114  TCCGGA               [AT]^CCGG[AT]
BseAI         114  TCCGGA               T^CCGGA
BsgI           19  GTGCAG               GTGCAG(16/14)
BsiWI         105  CGTACG               C^GTACG
BsmAI         976  GAGAC                GTCTC(1/5)
BsmFI         419  GGGAC                GGGAC(10/14)
BsmI         1179  GCATTC               GAATGC(1/-1)
BspEI         114  TCCGGA               T^CCGGA
BspMI          21  GCAGGT               ACCTGC(4/8)
BssSI           9  CTCGTG               CACGAG(-5/-1)
CpoI          111  CGGTCCG              CG^G[AT]CCG
DraIII        136  CACTTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI           71  CCGTGG               C^C[GA][CT]GG
EarI          679  GAAGAG               CTCTTC(1/4)
HapII         115  CCGG                 C^CGG
HindIII       171  AAGCTT               A^AGCTT
HpaII         115  CCGG                 C^CGG
Ksp632I       679  GAAGAG               CTCTTC(1/4)
MaeII        1000  ACGT                 A^CGT
MamI         1579  GATTTTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          251  CAATTG               C^AATTG
MroI          114  TCCGGA               T^CCGGA
MspA1I        351  CCGCTG               C[AC]G^C[GT]G
MspI          115  CCGG                 C^CGG
MunI          251  CAATTG               C^AATTG
NheI            4  GCTAGC               G^CTAGC
PshBI         543  ATTAAT               AT^TAAT
Psp1406I      999  AACGTT               AA^CGTT
PstI         1516  CTGCAG               CTGCA^G
RsrII         111  CGGTCCG              CG^G[AT]CCG
SfaNI         318  GCATC                GCATC(5/9)
SmlI         1287  CTTAAG               C^T[CT][GA]AG
StuI         1246  AGGCCT               AGG^CCT
Tsp45I       1445  GTGAC                ^GT[GC]AC
XmnI          169  GAAAGCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AccII          46  CGCG                 CG^CG
              109  CGCG                
AfaI          106  GTAC                 GT^AC
              518  GTAC                
AspHI         118  GAGCAC               G[AT]GC[AT]^C
              553  GAGCAC              
AvrII         178  CCTAGG               C^CTAGG
              283  CCTAGG              
BlnI          178  CCTAGG               C^CTAGG
              283  CCTAGG              
BsiHKAI       118  GAGCAC               G[AT]GC[AT]^C
              553  GAGCAC              
BstUI          46  CGCG                 CG^CG
              109  CGCG                
CfoI           47  GCGC                 GCG^C
              884  GCGC                
DraI          265  TTTAAA               TTT^AAA
              868  TTTAAA              
DraII         419  GGGACCC              [GA]G^G[ACGT]CC[CT]
             1149  AGGTCCT             
EcoO109I      419  GGGACCC              [GA]G^G[ACGT]CC[CT]
             1149  AGGTCCT             
EcoT14I       178  CCTAGG               C^C[AT][AT]GG
              283  CCTAGG              
HhaI           47  GCGC                 GCG^C
              884  GCGC                
HinP1I         47  GCGC                 G^CGC
              884  GCGC                
MslI          589  CATAAAAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1180  CATTCTGATG          
MvnI           46  CGCG                 CG^CG
              109  CGCG                
NlaIII        723  CATG                 CATG^
              943  CATG                
PleI          577  GACTC                GAGTC(4/5)
             1231  GACTC               
PpuMI         419  GGGACCC              [GA]G^G[AT]CC[CT]
             1149  AGGTCCT             
RsaI          106  GTAC                 GT^AC
              518  GTAC                
SfcI         1516  CTGCAG               C^T[GA][CT]AG
             1621  CTACAG              
StyI          178  CCTAGG               C^C[AT][AT]GG
              283  CCTAGG              


#########################################
# 3 OR MORE CUT ENZYMES (   49 enzymes) #
#########################################

AciI        AluI        AlwI        AvaII       BbvI        
BfaI        BmyI        BpmI        BsaJI       BseRI       
BsiYI       BslI        Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       Cfr13I      
DdeI        DpnII       EcoRII      Fnu4HI      FokI        
HaeIII      HinfI       HphI        ItaI        MaeI        
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MvaI        MwoI        NdeII       NlaIV       
Sau3AI      Sau96I      ScrFI       TfiI        Tru9I       
TseI        Tsp509I     TspRI       XhoII       
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