Restriction map for pF1KB8734
 (Linear) (Six-base) MAPSORT of: KIBB8734.seq  Check: 8463  from: 1  to: 1653



KIBB8734.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      12    1653 
   Size:         12    1641
 
AclI AA'CG_TT

Cuts at:      0    1000    1653 
   Size:       1000     653
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     427     839    1653 
   Size:        427     412     814

  Fragments arranged by size: 

                 814     427     412
 
AflII C'TTAA_G

Cuts at:      0    1287    1653 
   Size:       1287     366
 
AlwNI CAG_nnn'CTG

Cuts at:      0     817    1653 
   Size:        817     836
 
AseI AT'TA_AT

Cuts at:      0     544    1653 
   Size:        544    1109
 
AvrII C'CTAG_G

Cuts at:      0     178     283    1653 
   Size:        178     105    1370

  Fragments arranged by size: 

                1370     178     105
 
BamHI G'GATC_C

Cuts at:      0    1026    1653 
   Size:       1026     627
 
BanI G'GyrC_C

Cuts at:      0      41    1653 
   Size:         41    1612
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     147    1653 
   Size:        147    1506
 
BglII A'GATC_T

Cuts at:      0    1173    1653 
   Size:       1173     480
 
Bme1580I G_kGCm'C

Cuts at:      0      44    1653 
   Size:         44    1609
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1079    1653 
   Size:       1079     574
 
BmtI G_CTAG'C

Cuts at:      0       8    1653 
   Size:          8    1645
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     564    1083    1089    1095    1101    1653 
   Size:         52     512     519       6       6       6     552

  Fragments arranged by size: 

                 552     519     512      52       6       6       6
 
BsaBI GATnn'nnATC

Cuts at:      0    1583    1653 
   Size:       1583      70
 
BsaWI w'CCGG_w

Cuts at:      0     114    1653 
   Size:        114    1539
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1063    1210    1274    1653 
   Size:       1063     147      64     379

  Fragments arranged by size: 

                1063     379     147      64
 
BseYI C'CCAG_C

Cuts at:      0      36    1653 
   Size:         36    1617
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40    1653 
   Size:         40    1613
 
BsiHKAI G_wGCw'C

Cuts at:      0     122     557    1653 
   Size:        122     435    1096

  Fragments arranged by size: 

                1096     435     122
 
BsiWI C'GTAC_G

Cuts at:      0     105    1653 
   Size:        105    1548
 
BsmI GAATG_Cn'

Cuts at:      0    1179    1653 
   Size:       1179     474
 
Bsp1286I G_dGCh'C

Cuts at:      0      44     122     557    1653 
   Size:         44      78     435    1096

  Fragments arranged by size: 

                1096     435      78      44
 
BspEI T'CCGG_A

Cuts at:      0     114    1653 
   Size:        114    1539
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      12    1653 
   Size:         12    1641
 
BssSI C'ACGA_G

Cuts at:      0       9    1653 
   Size:          9    1644
 
BstYI r'GATC_y

Cuts at:      0     201    1026    1173    1653 
   Size:        201     825     147     480

  Fragments arranged by size: 

                 825     480     201     147
 
BtgI C'CryG_G

Cuts at:      0      71    1653 
   Size:         71    1582
 
DraI TTT'AAA

Cuts at:      0     267     870    1653 
   Size:        267     603     783

  Fragments arranged by size: 

                 783     603     267
 
DraIII CAC_nnn'GTG

Cuts at:      0     141    1653 
   Size:        141    1512
 
EarI CTCTTCn'nnn_

Cuts at:      0     674    1653 
   Size:        674     979
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     427     564     839    1083    1089    1095    1101 
   Size:         52     375     137     275     244       6       6       6

Cuts at:   1101    1653 
   Size:        552

  Fragments arranged by size: 

                 552     375     275     244     137      52       6       6
                   6
 
EcoO109I rG'GnC_Cy

Cuts at:      0     420    1150    1653 
   Size:        420     730     503

  Fragments arranged by size: 

                 730     503     420
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     720    1653 
   Size:        720     933
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1042    1074    1186    1216    1218    1248    1576    1608 
   Size:       1042      32     112      30       2      30     328      32

Cuts at:   1608    1653 
   Size:         45

  Fragments arranged by size: 

                1042     328     112      45      32      32      30      30
                   2
 
HindIII A'AGCT_T

Cuts at:      0     171    1653 
   Size:        171    1482
 
MfeI C'AATT_G

Cuts at:      0     251    1653 
   Size:        251    1402
 
MslI CAynn'nnrTG

Cuts at:      0     593    1184    1653 
   Size:        593     591     469

  Fragments arranged by size: 

                 593     591     469
 
MspA1I CmG'CkG

Cuts at:      0     353    1653 
   Size:        353    1300
 
NheI G'CTAG_C

Cuts at:      0       4    1653 
   Size:          4    1649
 
PpuMI rG'GwC_Cy

Cuts at:      0     420    1150    1653 
   Size:        420     730     503

  Fragments arranged by size: 

                 730     503     420
 
PstI C_TGCA'G

Cuts at:      0    1520    1653 
   Size:       1520     133
 
RsrII CG'GwC_CG

Cuts at:      0     112    1653 
   Size:        112    1541
 
SanDI GG'GwC_CC

Cuts at:      0     420    1653 
   Size:        420    1233
 
SfcI C'TryA_G

Cuts at:      0    1516    1621    1653 
   Size:       1516     105      32

  Fragments arranged by size: 

                1516     105      32
 
SmlI C'TyrA_G

Cuts at:      0    1287    1653 
   Size:       1287     366
 
StuI AGG'CCT

Cuts at:      0    1248    1653 
   Size:       1248     405
 
StyI C'CwwG_G

Cuts at:      0     178     283    1653 
   Size:        178     105    1370

  Fragments arranged by size: 

                1370     178     105
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     100    1653 
   Size:        100    1553
 
XmnI GAAnn'nnTTC

Cuts at:      0     173    1653 
   Size:        173    1480
 

 Enzymes that do cut:

     AarI     AclI     AcuI    AflII    AlwNI     AseI    AvrII    BamHI
     BanI     BbsI    BglII Bme1580I     BmrI     BmtI     BpmI    BsaBI
    BsaWI    BseRI    BseYI     BsgI  BsiHKAI    BsiWI     BsmI Bsp1286I
    BspEI    BspMI    BssSI    BstYI     BtgI     DraI   DraIII     EarI
  Eco57MI EcoO109I     FalI    Hin4I  HindIII     MfeI     MslI   MspA1I
     NheI    PpuMI     PstI    RsrII    SanDI     SfcI     SmlI     StuI
     StyI    TaqII     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AfeI   AflIII     AgeI     AhdI     AleI
     AlfI     AloI     ApaI    ApaLI     ApoI     AscI    AsiSI     AvaI
     BaeI    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI     BlpI    BmgBI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaHI    BsaXI    BsiEI    BsmBI    BspHI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII
   HincII     HpaI     KasI     KpnI     MluI     MmeI     MscI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII     SacI
    SacII     SalI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com