Restriction map for pF1KB8732
##################################
# NO CUT ENZYMES (  117 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AflIII      AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       AscI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       BalI        BbeI        BbrPI       BbsI        
BcgI        BciVI       BfrI        BglII       BpuAI       
BsaAI       BsaBI       BsaWI       BseAI       BsgI        
BsiHKAI     BsiWI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrGI       BssHII      Bst1107I    BstEII      
BstPI       BstXI       BstZ17I     Bsu36I      ClaI        
CpoI        DraI        DraIII      Eam1105I    Eco47III    
Eco81I      EcoO65I     EcoRV       FseI        FspI        
HindIII     HpaI        KasI        KpnI        MaeII       
MamI        MluI        MluNI       MroI        MscI        
NaeI        NarI        NgoMIV      NheI        NotI        
NruI        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SgrAI       SmaI        SnaBI       SpeI        Sse8387I    
SspBI       SspI        StuI        SwaI        TfiI        
Tth111I     XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccI         1044  GTCGAC               GT^[AC][GT]AC
AcyI         1041  GGCGTC               G[GA]^CG[CT]C
AseI         1501  ATTAAT               AT^TAAT
AsnI         1501  ATTAAT               AT^TAAT
BamHI         444  GGATCC               G^GATCC
BanI          574  GGTGCC               G^G[CT][GA]CC
BclI          455  TGATCA               T^GATCA
BcnI           29  CCGGG                CC^[GC]GG
BglI          952  GCCCTGGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI        1041  GGCGTC               G[GA]^CG[CT]C
BsaI          852  GGTCTC               GGTCTC(1/5)
BsiEI          26  CGGCCG               CG[GA][CT]^CG
BsrFI         437  ACCGGC               [GA]^CCGG[CT]
BssSI         364  CTCGTG               CACGAG(-5/-1)
BstBI         190  TTCGAA               TT^CGAA
Cfr10I        437  ACCGGC               [GA]^CCGG[CT]
DrdI         1035  GACCCTGGCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI           26  CGGCCG               [CT]^GGCC[GA]
EagI           26  CGGCCG               C^GGCCG
EarI          652  GAAGAG               CTCTTC(1/4)
EclXI          26  CGGCCG               C^GGCCG
Eco52I         26  CGGCCG               C^GGCCG
EcoNI         229  CCTGCCCTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I      1405  ATGCAT               ATGCA^T
FauI           19  CCCGC                CCCGC(4/6)
FbaI          455  TGATCA               T^GATCA
HgaI         1042  GCGTC                GACGC(5/10)
Hin1I        1041  GGCGTC               G[GA]^CG[CT]C
HincII       1044  GTCGAC               GT[CT]^[GA]AC
HindII       1044  GTCGAC               GT[CT]^[GA]AC
Ksp632I       652  GAAGAG               CTCTTC(1/4)
KspI           20  CCGCGG               CCGC^GG
MfeI         1330  CAATTG               C^AATTG
MunI         1330  CAATTG               C^AATTG
NciI           29  CCGGG                CC^[GC]GG
NsiI         1405  ATGCAT               ATGCA^T
NspV          190  TTCGAA               TT^CGAA
PpuMI          32  GGGACCC              [GA]G^G[AT]CC[CT]
PshBI        1501  ATTAAT               AT^TAAT
SacII          20  CCGCGG               CCGC^GG
SalI         1044  GTCGAC               G^TCGAC
SfuI          190  TTCGAA               TT^CGAA
SphI          239  GCATGC               GCATG^C


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AvrII         234  CCTAGG               C^CTAGG
             1138  CCTAGG              
BanII        1235  GGGCTC               G[GA]GC[CT]^C
             1644  GAGCCC              
BbvCI         270  CCTCAGC              CCTCAGC(-5/-2)
              118  GCTGAGG             
BfaI          235  CTAG                 C^TAG
             1139  CTAG                
BlnI          234  CCTAGG               C^CTAGG
             1138  CCTAGG              
BlpI          543  GCTCAGC              GC^T[ACGT]AGC
             1274  GCTCAGC             
BmyI         1235  GGGCTC               G[AGT]GC[ACT]^C
             1644  GAGCCC              
Bpu1102I      543  GCTCAGC              GC^T[ACGT]AGC
             1274  GCTCAGC             
BseRI         212  CTCCTC               GAGGAG(10/8)
             1586  CTCCTC              
Bsp1286I     1235  GGGCTC               G[AGT]GC[ACT]^C
             1644  GAGCCC              
BsrDI         281  CATTGC               GCAATG(2/0)
             1206  CATTGC              
BstYI         444  GGATCC               [GA]^GATC[CT]
              750  AGATCC              
CelII         543  GCTCAGC              GC^T[ACGT]AGC
             1274  GCTCAGC             
DraII          32  GGGACCC              [GA]G^G[ACGT]CC[CT]
              950  AGGCCCT             
EcoO109I       32  GGGACCC              [GA]G^G[ACGT]CC[CT]
              950  AGGCCCT             
EcoRI         187  GAATTC               G^AATTC
              301  GAATTC              
HaeII         547  AGCGCC               [GA]GCGC^[CT]
             1535  AGCGCC              
MaeI          235  CTAG                 C^TAG
             1139  CTAG                
MaeIII       1192  GTCAC                ^GT[ACGT]AC
             1201  GTCAC               
MflI          444  GGATCC               [GA]^GATC[CT]
              750  AGATCC              
NcoI         1253  CCATGG               C^CATGG
             1265  CCATGG              
NdeI          403  CATATG               CA^TATG
              782  CATATG              
PflMI         169  CCAAGCCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              703  CCAGACTCTGG         
SmlI          354  CTCAAG               C^T[CT][GA]AG
             1700  CTCAAG              
Tsp45I       1192  GTCAC                ^GT[GC]AC
             1201  GTCAC               
Van91I        169  CCAAGCCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              703  CCAGACTCTGG         
XhoII         444  GGATCC               [GA]^GATC[CT]
              750  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   71 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        AvaII       BbvI        
BpmI        BsaJI       BsiYI       BslI        BsmAI       
BsmFI       BspMI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DsaI        EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        MboI        MboII       MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NdeII       NlaIII      NlaIV       NspI        
PleI        PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StyI        
TaqI        Tru9I       TseI        Tsp509I     TspRI       
TthHB8I     
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