Restriction map for
pF1KB8732
(Circular) (Six-base) MAPSORT of: pF1KB8732.seq Check: 1246 from: 1 to: 4841
pF1KB8732 4841 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1583 1583
Size: 4841
AccI GT'mk_AC
Cuts at: 1153 1879 1153
Size: 726 4115
Acc65I G'GTAC_C
Cuts at: 1857 1857
Size: 4841
AclI AA'CG_TT
Cuts at: 4681 4681
Size: 4841
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1075 2666 3098 3451 4113 1075
Size: 1591 432 353 662 1803
Fragments arranged by size:
1803 1591 662 432 353
AflIII A'CryG_T
Cuts at: 2193 3993 4274 2193
Size: 1800 281 2760
Fragments arranged by size:
2760 1800 281
AleI CACnn'nnGTG
Cuts at: 532 1399 532
Size: 867 3974
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 523 523
Size: 4841
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 103 135 103
Size: 32 4809
AlwNI CAG_nnn'CTG
Cuts at: 171 337 817 1036 1146 1575 1623 1635 1662
Size: 166 480 219 110 429 48 12 27
Cuts at: 1662 2146 3584 171
Size: 484 1438 1428
Fragments arranged by size:
1438 1428 484 480 429 219 166 110
48 27 12
ApaLI G'TGCA_C
Cuts at: 3679 3679
Size: 4841
ApoI r'AATT_y
Cuts at: 295 302 409 672 1845 4194 4450 295
Size: 7 107 263 1173 2349 256 686
Fragments arranged by size:
2349 1173 686 263 256 107 7
AseI AT'TA_AT
Cuts at: 20 1610 20
Size: 1590 3251
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4841
AvaI C'yCGr_G
Cuts at: 1861 1861
Size: 4841
AvrII C'CTAG_G
Cuts at: 342 1246 342
Size: 904 3937
BamHI G'GATC_C
Cuts at: 552 1866 552
Size: 1314 3527
BanI G'GyrC_C
Cuts at: 682 1857 2271 2519 2554 682
Size: 1175 414 248 35 2969
Fragments arranged by size:
2969 1175 414 248 35
BanII G_rGCy'C
Cuts at: 1347 1756 1855 2885 1347
Size: 409 99 1030 3303
Fragments arranged by size:
3303 1030 409 99
BbeI G_GCGC'C
Cuts at: 2275 2523 2275
Size: 248 4593
BbvCI CC'TCA_GC
Cuts at: 227 379 227
Size: 152 4689
BciVI GTATCCnnnnn_n'
Cuts at: 2739 3795 4841 2739
Size: 1056 1046 2739
Fragments arranged by size:
2739 1056 1046
BclI T'GATC_A
Cuts at: 563 2361 563
Size: 1798 3043
BfrBI ATG'CAT
Cuts at: 1515 1515
Size: 4841
BglI GCCn_nnn'nGGC
Cuts at: 1066 1066
Size: 4841
BglII A'GATC_T
Cuts at: 2356 4393 2356
Size: 2037 2804
BlpI GC'TnA_GC
Cuts at: 652 1383 1953 652
Size: 731 570 3540
Fragments arranged by size:
3540 731 570
Bme1580I G_kGCm'C
Cuts at: 2466 2559 3683 2466
Size: 93 1124 3624
Fragments arranged by size:
3624 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 414 534 654 2053 2134 2469 4613 414
Size: 120 120 1399 81 335 2144 642
Fragments arranged by size:
2144 1399 642 335 120 120 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3208 3208
Size: 4841
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 308 698 794 1216 1483 1582 1633 308
Size: 390 96 422 267 99 51 3516
Fragments arranged by size:
3516 422 390 267 99 96 51
Bpu10I CC'TnA_GC
Cuts at: 227 379 227
Size: 152 4689
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 447 1793 2017 2896 3610 3908 447
Size: 1346 224 879 714 298 1380
Fragments arranged by size:
1380 1346 879 714 298 224
BsaI GGTCTCn'nnnn_
Cuts at: 966 4545 966
Size: 3579 1262
BsaAI yAC'GTr
Cuts at: 2194 2194
Size: 4841
BsaBI GATnn'nnATC
Cuts at: 6 2355 4392 6
Size: 2349 2037 455
Fragments arranged by size:
2349 2037 455
BsaHI Gr'CG_yC
Cuts at: 1150 2272 2520 3222 4718 1150
Size: 1122 248 702 1496 1273
Fragments arranged by size:
1496 1273 1122 702 248
BsaWI w'CCGG_w
Cuts at: 2030 2551 3229 3640 3787 4061 2030
Size: 521 678 411 147 274 2810
Fragments arranged by size:
2810 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 306 336 306
Size: 30 4811
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 311 1685 311
Size: 1374 3467
BseYI C'CCAG_C
Cuts at: 623 1421 1757 2266 3024 3689 4097 623
Size: 798 336 509 758 665 408 1367
Fragments arranged by size:
1367 798 758 665 509 408 336
BsiEI CG_ry'CG
Cuts at: 104 137 2383 2429 3659 104
Size: 33 2246 46 1230 1286
Fragments arranged by size:
2246 1286 1230 46 33
BsiHKAI G_wGCw'C
Cuts at: 1855 2633 2823 3683 1855
Size: 778 190 860 3013
Fragments arranged by size:
3013 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 1347 1756 1855 2466 2559 2633 2823 2885 3683
Size: 409 99 611 93 74 190 62 798
Cuts at: 3683 1347
Size: 2505
Fragments arranged by size:
2505 798 611 409 190 99 93 74
62
BspHI T'CATG_A
Cuts at: 3273 3273
Size: 4841
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1002 1583 1812 1891 2105 2407 2788 1002
Size: 581 229 79 214 302 381 3055
Fragments arranged by size:
3055 581 381 302 229 214 79
BsrBI CCG'CTC
Cuts at: 50 3133 3187 4671 4836 50
Size: 3083 54 1484 165 55
Fragments arranged by size:
3083 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 388 1313 2753 388
Size: 925 1440 2476
Fragments arranged by size:
2476 1440 925
BsrFI r'CCGG_y
Cuts at: 545 2839 3020 545
Size: 2294 181 2366
Fragments arranged by size:
2366 2294 181
BssSI C'ACGA_G
Cuts at: 472 3112 3820 472
Size: 2640 708 1493
Fragments arranged by size:
2640 1493 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1434 1482 1512 1536 1563 1575 1635 1662 1671
Size: 48 30 24 27 12 60 27 9
Cuts at: 1671 2218 1434
Size: 547 4057
Fragments arranged by size:
4057 547 60 48 30 27 27 24
12 9
BstBI TT'CG_AA
Cuts at: 299 3202 299
Size: 2903 1938
BstYI r'GATC_y
Cuts at: 1 552 858 1866 2333 2356 2691 2937 3243
Size: 551 306 1008 467 23 335 246 306
Cuts at: 3243 3255 3341 4393 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 1008 551 467 449 335 306 306
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 128 1361 1373 107
Size: 21 1233 12 3575
Fragments arranged by size:
3575 1233 21 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2791 2972 3135 2791
Size: 181 163 4497
Fragments arranged by size:
4497 181 163
BtsI GCAGTG_nn'
Cuts at: 172 507 2084 172
Size: 335 1577 2929
Fragments arranged by size:
2929 1577 335
DraI TTT'AAA
Cuts at: 1840 1840
Size: 4841
DrdI GACnn_nn'nnGTC
Cuts at: 1149 2547 3891 1149
Size: 1398 1344 2099
Fragments arranged by size:
2099 1398 1344
EaeI y'GGCC_r
Cuts at: 134 2426 2600 2991 3018 4363 134
Size: 2292 174 391 27 1345 612
Fragments arranged by size:
2292 1345 612 391 174 27
EagI C'GGCC_G
Cuts at: 134 2426 134
Size: 2292 2549
EarI CTCTTCn'nnn_
Cuts at: 755 2864 3074 755
Size: 2109 210 2522
Fragments arranged by size:
2522 2109 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3049 3791 3937 4651 3049
Size: 742 146 714 3239
Fragments arranged by size:
3239 742 714 146
EcoICRI GAG'CTC
Cuts at: 1853 1853
Size: 4841
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 308 698 794 1075 1216 1483 1582 1633 2666
Size: 390 96 281 141 267 99 51 1033
Cuts at: 2666 3098 3451 4113 308
Size: 432 353 662 1036
Fragments arranged by size:
1036 1033 662 432 390 353 281 267
141 99 96 51
EcoNI CCTnn'n_nnAGG
Cuts at: 341 341
Size: 4841
EcoO109I rG'GnC_Cy
Cuts at: 141 1059 1980 141
Size: 918 921 3002
Fragments arranged by size:
3002 921 918
EcoRI G'AATT_C
Cuts at: 295 409 1845 295
Size: 114 1436 3291
Fragments arranged by size:
3291 1436 114
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2860 2860
Size: 4841
FspI TGC'GCA
Cuts at: 2622 2622
Size: 4841
HaeII r_GCGC'y
Cuts at: 659 1647 2275 2523 3753 4524 659
Size: 988 628 248 1230 771 976
Fragments arranged by size:
1230 988 976 771 628 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1791 1823 2683 2713 2715 2745 1791
Size: 32 860 30 2 30 3887
Fragments arranged by size:
3887 860 32 30 30 2
HincII GTy'rAC
Cuts at: 1154 1880 1154
Size: 726 4115
KasI G'GCGC_C
Cuts at: 2271 2519 2271
Size: 248 4593
KpnI G_GTAC'C
Cuts at: 1861 1861
Size: 4841
MfeI C'AATT_G
Cuts at: 1438 1438
Size: 4841
MluI A'CGCG_T
Cuts at: 4274 4274
Size: 4841
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3600 3784 3600
Size: 184 4657
MscI TGG'CCA
Cuts at: 2602 2602
Size: 4841
MslI CAynn'nnrTG
Cuts at: 112 500 532 981 1399 2830 2957 112
Size: 388 32 449 418 1431 127 1996
Fragments arranged by size:
1996 1431 449 418 388 127 32
MspA1I CmG'CkG
Cuts at: 130 1623 1635 1653 1662 1952 2266 2626 3410
Size: 1493 12 18 9 290 314 360 784
Cuts at: 3410 3655 130
Size: 245 1316
Fragments arranged by size:
1493 1316 784 360 314 290 245 18
12 9
NaeI GCC'GGC
Cuts at: 3022 3022
Size: 4841
NarI GG'CG_CC
Cuts at: 2272 2520 2272
Size: 248 4593
NcoI C'CATG_G
Cuts at: 107 1361 1373 107
Size: 1254 12 3575
Fragments arranged by size:
3575 1254 12
NdeI CA'TA_TG
Cuts at: 512 891 512
Size: 379 4462
NgoMIV G'CCGG_C
Cuts at: 3020 3020
Size: 4841
NsiI A_TGCA'T
Cuts at: 1517 1517
Size: 4841
NspI r_CATG'y
Cuts at: 351 1329 1566 1674 1894 2394 3997 351
Size: 978 237 108 220 500 1603 1195
Fragments arranged by size:
1603 1195 978 500 237 220 108
PasI CC'CwG_GG
Cuts at: 399 399
Size: 4841
PciI A'CATG_T
Cuts at: 3993 3993
Size: 4841
PflMI CCAn_nnn'nTGG
Cuts at: 283 817 283
Size: 534 4307
PmeI GTTT'AAAC
Cuts at: 1840 1840
Size: 4841
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3287 3319 3287
Size: 32 4809
PpuMI rG'GwC_Cy
Cuts at: 141 141
Size: 4841
PstI C_TGCA'G
Cuts at: 1580 1628 1640 1658 1888 2573 1580
Size: 48 12 18 230 685 3848
Fragments arranged by size:
3848 685 230 48 18 12
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4841
PvuII CAG'CTG
Cuts at: 1623 1635 1653 1662 2266 2626 1623
Size: 12 18 9 604 360 3838
Fragments arranged by size:
3838 604 360 18 12 9
RsrII CG'GwC_CG
Cuts at: 3036 3036
Size: 4841
SacI G_AGCT'C
Cuts at: 1855 1855
Size: 4841
SacII CC_GC'GG
Cuts at: 131 131
Size: 4841
SalI G'TCGA_C
Cuts at: 1152 1878 1152
Size: 726 4115
SanDI GG'GwC_CC
Cuts at: 141 141
Size: 4841
SapI GCTCTTCn'nnn_
Cuts at: 2864 3074 2864
Size: 210 4631
SbfI CC_TGCA'GG
Cuts at: 1888 1888
Size: 4841
ScaI AGT'ACT
Cuts at: 4358 4358
Size: 4841
SfcI C'TryA_G
Cuts at: 33 186 1576 1624 1636 1654 1884 2569 3537
Size: 153 1390 48 12 18 230 685 968
Cuts at: 3537 3728 33
Size: 191 1146
Fragments arranged by size:
1390 1146 968 685 230 191 153 48
18 12
SfoI GGC'GCC
Cuts at: 2273 2521 2273
Size: 248 4593
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4841
SmaI CCC'GGG
Cuts at: 1863 1863
Size: 4841
SmlI C'TyrA_G
Cuts at: 462 1808 1996 2911 3625 3887 462
Size: 1346 188 915 714 262 1416
Fragments arranged by size:
1416 1346 915 714 262 188
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4841
SphI G_CATG'C
Cuts at: 351 1894 2394 351
Size: 1543 500 2798
Fragments arranged by size:
2798 1543 500
StyI C'CwwG_G
Cuts at: 107 282 342 993 1246 1361 1373 1975 2329
Size: 175 60 651 253 115 12 602 354
Cuts at: 2329 107
Size: 2619
Fragments arranged by size:
2619 651 602 354 253 175 115 60
12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 604 1411 2424 2557 3016 3225 604
Size: 807 1013 133 459 209 2220
Fragments arranged by size:
2220 1013 807 459 209 133
TatI w'GTAC_w
Cuts at: 357 4356 357
Size: 3999 842
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 306 338 4358 4390 306
Size: 32 4020 32 757
Fragments arranged by size:
4020 757 32 32
Tth111I GACn'n_nGTC
Cuts at: 2638 2638
Size: 4841
XbaI T'CTAG_A
Cuts at: 1872 1872
Size: 4841
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2336 2336
Size: 4841
XmaI C'CCGG_G
Cuts at: 1861 1861
Size: 4841
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbvCI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PasI
PciI PflMI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI ApaI AscI BaeI
BbsI BcgI BmgBI BmtI BsgI BsiWI BsmI BsmBI
BspEI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI NheI
NotI NruI PacI PfoI PmlI PshAI PsiI PspOMI
PspXI PsrI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI