Restriction map for pF1KB8732
 (Linear) (Six-base) MAPSORT of: KIBB8732.seq  Check: 717  from: 1  to: 1728



KIBB8732.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1475    1728 
   Size:       1475     253
 
AccI GT'mk_AC

Cuts at:      0    1045    1728 
   Size:       1045     683
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     967    1728 
   Size:        967     761
 
AleI CACnn'nnGTG

Cuts at:      0     424    1291    1728 
   Size:        424     867     437

  Fragments arranged by size: 

                 867     437     424
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     415    1728 
   Size:        415    1313
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      27    1728 
   Size:         27    1701
 
AlwNI CAG_nnn'CTG

Cuts at:      0      63     229     709     928    1038    1467    1515    1527 
   Size:         63     166     480     219     110     429      48      12

Cuts at:   1527    1554    1728 
   Size:         27     174

  Fragments arranged by size: 

                 480     429     219     174     166     110      63      48
                  27      12
 
ApoI r'AATT_y

Cuts at:      0     187     194     301     564    1728 
   Size:        187       7     107     263    1164

  Fragments arranged by size: 

                1164     263     187     107       7
 
AseI AT'TA_AT

Cuts at:      0    1502    1728 
   Size:       1502     226
 
AvrII C'CTAG_G

Cuts at:      0     234    1138    1728 
   Size:        234     904     590

  Fragments arranged by size: 

                 904     590     234
 
BamHI G'GATC_C

Cuts at:      0     444    1728 
   Size:        444    1284
 
BanI G'GyrC_C

Cuts at:      0     574    1728 
   Size:        574    1154
 
BanII G_rGCy'C

Cuts at:      0    1239    1648    1728 
   Size:       1239     409      80

  Fragments arranged by size: 

                1239     409      80
 
BbvCI CC'TCA_GC

Cuts at:      0     119     271    1728 
   Size:        119     152    1457

  Fragments arranged by size: 

                1457     152     119
 
BclI T'GATC_A

Cuts at:      0     455    1728 
   Size:        455    1273
 
BfrBI ATG'CAT

Cuts at:      0    1407    1728 
   Size:       1407     321
 
BglI GCCn_nnn'nGGC

Cuts at:      0     958    1728 
   Size:        958     770
 
BlpI GC'TnA_GC

Cuts at:      0     544    1275    1728 
   Size:        544     731     453

  Fragments arranged by size: 

                 731     544     453
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     306     426     546    1728 
   Size:        306     120     120    1182

  Fragments arranged by size: 

                1182     306     120     120
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     200     590     686    1108    1375    1474    1525    1728 
   Size:        200     390      96     422     267      99      51     203

  Fragments arranged by size: 

                 422     390     267     203     200      99      96      51
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     119     271    1728 
   Size:        119     152    1457

  Fragments arranged by size: 

                1457     152     119
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     339    1685    1728 
   Size:        339    1346      43

  Fragments arranged by size: 

                1346     339      43
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     858    1728 
   Size:        858     870
 
BsaHI Gr'CG_yC

Cuts at:      0    1042    1728 
   Size:       1042     686
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     198     228    1728 
   Size:        198      30    1500

  Fragments arranged by size: 

                1500     198      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     203    1577    1728 
   Size:        203    1374     151

  Fragments arranged by size: 

                1374     203     151
 
BseYI C'CCAG_C

Cuts at:      0     515    1313    1649    1728 
   Size:        515     798     336      79

  Fragments arranged by size: 

                 798     515     336      79
 
BsiEI CG_ry'CG

Cuts at:      0      29    1728 
   Size:         29    1699
 
Bsp1286I G_dGCh'C

Cuts at:      0    1239    1648    1728 
   Size:       1239     409      80

  Fragments arranged by size: 

                1239     409      80
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     894    1475    1704    1728 
   Size:        894     581     229      24

  Fragments arranged by size: 

                 894     581     229      24
 
BsrDI GCAATG_nn'

Cuts at:      0     280    1205    1728 
   Size:        280     925     523

  Fragments arranged by size: 

                 925     523     280
 
BsrFI r'CCGG_y

Cuts at:      0     437    1728 
   Size:        437    1291
 
BssSI C'ACGA_G

Cuts at:      0     364    1728 
   Size:        364    1364
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1326    1374    1404    1428    1455    1467    1527    1554 
   Size:       1326      48      30      24      27      12      60      27

Cuts at:   1554    1563    1728 
   Size:          9     165

  Fragments arranged by size: 

                1326     165      60      48      30      27      27      24
                  12       9
 
BstBI TT'CG_AA

Cuts at:      0     191    1728 
   Size:        191    1537
 
BstYI r'GATC_y

Cuts at:      0     444     750    1728 
   Size:        444     306     978

  Fragments arranged by size: 

                 978     444     306
 
BtgI C'CryG_G

Cuts at:      0      20    1253    1265    1728 
   Size:         20    1233      12     463

  Fragments arranged by size: 

                1233     463      20      12
 
BtsI GCAGTG_nn'

Cuts at:      0      64     399    1728 
   Size:         64     335    1329

  Fragments arranged by size: 

                1329     335      64
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1041    1728 
   Size:       1041     687
 
EaeI y'GGCC_r

Cuts at:      0      26    1728 
   Size:         26    1702
 
EagI C'GGCC_G

Cuts at:      0      26    1728 
   Size:         26    1702
 
EarI CTCTTCn'nnn_

Cuts at:      0     647    1728 
   Size:        647    1081
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     200     590     686     967    1108    1375    1474    1525 
   Size:        200     390      96     281     141     267      99      51

Cuts at:   1525    1728 
   Size:        203

  Fragments arranged by size: 

                 390     281     267     203     200     141      99      96
                  51
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     233    1728 
   Size:        233    1495
 
EcoO109I rG'GnC_Cy

Cuts at:      0      33     951    1728 
   Size:         33     918     777

  Fragments arranged by size: 

                 918     777      33
 
EcoRI G'AATT_C

Cuts at:      0     187     301    1728 
   Size:        187     114    1427

  Fragments arranged by size: 

                1427     187     114
 
HaeII r_GCGC'y

Cuts at:      0     551    1539    1728 
   Size:        551     988     189

  Fragments arranged by size: 

                 988     551     189
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1683    1715    1728 
   Size:       1683      32      13

  Fragments arranged by size: 

                1683      32      13
 
HincII GTy'rAC

Cuts at:      0    1046    1728 
   Size:       1046     682
 
MfeI C'AATT_G

Cuts at:      0    1330    1728 
   Size:       1330     398
 
MslI CAynn'nnrTG

Cuts at:      0     392     424     873    1291    1728 
   Size:        392      32     449     418     437

  Fragments arranged by size: 

                 449     437     418     392      32
 
MspA1I CmG'CkG

Cuts at:      0      22    1515    1527    1545    1554    1728 
   Size:         22    1493      12      18       9     174

  Fragments arranged by size: 

                1493     174      22      18      12       9
 
NcoI C'CATG_G

Cuts at:      0    1253    1265    1728 
   Size:       1253      12     463

  Fragments arranged by size: 

                1253     463      12
 
NdeI CA'TA_TG

Cuts at:      0     404     783    1728 
   Size:        404     379     945

  Fragments arranged by size: 

                 945     404     379
 
NsiI A_TGCA'T

Cuts at:      0    1409    1728 
   Size:       1409     319
 
NspI r_CATG'y

Cuts at:      0     243    1221    1458    1566    1728 
   Size:        243     978     237     108     162

  Fragments arranged by size: 

                 978     243     237     162     108
 
PasI CC'CwG_GG

Cuts at:      0     291    1728 
   Size:        291    1437
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     175     709    1728 
   Size:        175     534    1019

  Fragments arranged by size: 

                1019     534     175
 
PpuMI rG'GwC_Cy

Cuts at:      0      33    1728 
   Size:         33    1695
 
PstI C_TGCA'G

Cuts at:      0    1472    1520    1532    1550    1728 
   Size:       1472      48      12      18     178

  Fragments arranged by size: 

                1472     178      48      18      12
 
PvuII CAG'CTG

Cuts at:      0    1515    1527    1545    1554    1728 
   Size:       1515      12      18       9     174

  Fragments arranged by size: 

                1515     174      18      12       9
 
SacII CC_GC'GG

Cuts at:      0      23    1728 
   Size:         23    1705
 
SalI G'TCGA_C

Cuts at:      0    1044    1728 
   Size:       1044     684
 
SanDI GG'GwC_CC

Cuts at:      0      33    1728 
   Size:         33    1695
 
SfcI C'TryA_G

Cuts at:      0      78    1468    1516    1528    1546    1728 
   Size:         78    1390      48      12      18     182

  Fragments arranged by size: 

                1390     182      78      48      18      12
 
SmlI C'TyrA_G

Cuts at:      0     354    1700    1728 
   Size:        354    1346      28

  Fragments arranged by size: 

                1346     354      28
 
SphI G_CATG'C

Cuts at:      0     243    1728 
   Size:        243    1485
 
StyI C'CwwG_G

Cuts at:      0     174     234     885    1138    1253    1265    1728 
   Size:        174      60     651     253     115      12     463

  Fragments arranged by size: 

                 651     463     253     174     115      60      12
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     496    1303    1728 
   Size:        496     807     425

  Fragments arranged by size: 

                 807     496     425
 
TatI w'GTAC_w

Cuts at:      0     249    1728 
   Size:        249    1479
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     198     230    1728 
   Size:        198      32    1498

  Fragments arranged by size: 

                1498     198      32
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AleI     AlfI     AloI    AlwNI     ApoI
     AseI    AvrII    BamHI     BanI    BanII    BbvCI     BclI    BfrBI
     BglI     BlpI     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaHI
    BsaXI    BseRI    BseYI    BsiEI Bsp1286I    BspMI    BsrDI    BsrFI
    BssSI   BstAPI    BstBI    BstYI     BtgI     BtsI     DrdI     EaeI
     EagI     EarI  Eco57MI    EcoNI EcoO109I    EcoRI    HaeII    Hin4I
   HincII     MfeI     MslI   MspA1I     NcoI     NdeI     NsiI     NspI
     PasI    PflMI    PpuMI     PstI    PvuII    SacII     SalI    SanDI
     SfcI     SmlI     SphI     StyI    TaqII     TatI     TstI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     ApaI    ApaLI     AscI    AsiSI     AvaI     BaeI     BbeI     BbsI
     BcgI    BciVI    BglII Bme1580I    BmgBI     BmtI     BplI    BsaAI
    BsaBI    BsaWI     BsgI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrBI    BsrGI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI     DraI   DraIII     EciI  EcoICRI    EcoRV
     FalI     FseI     FspI    FspAI  HindIII     HpaI     KasI     KpnI
     MluI     MmeI     MscI     NaeI     NarI   NgoMIV     NheI     NotI
     NruI     PacI     PciI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SrfI     SspI     StuI     SwaI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI
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