Restriction map for pF1KB8727
##################################
# NO CUT ENZYMES (  105 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AfaI        
AflII       AgeI        Alw44I      Aor51HI     ApaLI       
AscI        AseI        AsnI        Asp718I     AspI        
AvrII       BamHI       BbeI        BcgI        BciVI       
BclI        BfaI        BfrI        BlnI        BlpI        
Bpu1102I    BsaBI       BsaI        BseAI       BsgI        
BsiEI       BsiWI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BsrBI       BsrDI       BsrGI       
BssHII      Bst1107I    BstAPI      BstBI       BstZ17I     
CelII       ClaI        DraI        DrdI        EagI        
EclXI       Eco47III    Eco52I      EcoRI       EcoRV       
EcoT22I     FbaI        FseI        HindIII     HpaI        
KasI        KpnI        MaeI        MamI        MfeI        
MroI        MseI        MunI        NarI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsaI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmlI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
SwaI        Tru9I       Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AccI         1079  GTCGAC               GT^[AC][GT]AC
AcyI         1198  GACGCC               G[GA]^CG[CT]C
AhdI          469  GACATGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI         1224  GATCC                GGATC(4/5)
AocI         1303  CCTAAGG              CC^T[ACGT]AGG
Asp700I        18  GAACCCGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         469  GACATGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI         1404  CCCGGG               C^[CT]CG[GA]G
AviII         659  TGCGCA               TGC^GCA
BbsI         1437  GAAGAC               GAAGAC(2/6)
BglI          946  GCCGAGCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         104  AGATCT               A^GATCT
BpuAI        1437  GAAGAC               GAAGAC(2/6)
BsaHI        1198  GACGCC               G[GA]^CG[CT]C
BsaWI         613  TCCGGT               [AT]^CCGG[AT]
BseRI         844  GAGGAG               GAGGAG(10/8)
BsmBI         124  GAGACG               CGTCTC(1/5)
BsoBI        1404  CCCGGG               C^[CT]CG[GA]G
BspLU11I     1100  ACATGT               A^CATGT
BspMI         428  ACCTGC               ACCTGC(4/8)
BssSI         193  CACGAG               CACGAG(-5/-1)
BstXI        1274  CCACTGACGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         104  AGATCT               [GA]^GATC[CT]
Bsu36I       1303  CCTAAGG              CC^T[ACGT]AGG
CpoI         1361  CGGACCG              CG^G[AT]CCG
DraIII        193  CACGAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI         1358  CCGCGG               C^C[GA][CT]GG
Eam1105I      469  GACATGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       1303  CCTAAGG              CC^T[ACGT]AGG
EcoNI        1245  CCTCATGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       929  CCAAGG               C^C[AT][AT]GG
FspI          659  TGCGCA               TGC^GCA
HaeII        1425  AGCGCC               [GA]GCGC^[CT]
Hin1I        1198  GACGCC               G[GA]^CG[CT]C
HincII       1079  GTCGAC               GT[CT]^[GA]AC
HindII       1079  GTCGAC               GT[CT]^[GA]AC
KspI         1358  CCGCGG               CCGC^GG
MflI          104  AGATCT               [GA]^GATC[CT]
MluI          377  ACGCGT               A^CGCGT
NaeI          951  GCCGGC               GCC^GGC
NgoMIV        951  GCCGGC               G^CCGGC
PflMI         255  CCACGTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsrII        1361  CGGACCG              CG^G[AT]CCG
SacII        1358  CCGCGG               CCGC^GG
SalI         1079  GTCGAC               G^TCGAC
SapI         1297  GAAGAGC              GCTCTTC(1/4)
SmaI         1404  CCCGGG               CCC^GGG
SphI          579  GCATGC               GCATG^C
StyI          929  CCAAGG               C^C[AT][AT]GG
Van91I        255  CCACGTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          455  CCATGACTGACCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         104  AGATCT               [GA]^GATC[CT]
XmaCI        1404  CCCGGG               C^CCGGG
XmaI         1404  CCCGGG               C^CCGGG
XmnI           18  GAACCCGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AcsI         1324  GAATTT               [GA]^AATT[CT]
             1333  AAATTC              
AflIII        377  ACGCGT               A^C[GA][CT]GT
             1100  ACATGT              
AlwNI        1060  CAGTCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1264  CAGTGGCTG           
ApoI         1324  GAATTT               [GA]^AATT[CT]
             1333  AAATTC              
BalI          266  TGGCCA               TGG^CCA
              573  TGGCCA              
BanI          245  GGCACC               G^G[CT][GA]CC
              723  GGTGCC              
BbrPI         256  CACGTG               CAC^GTG
              777  CACGTG              
BbvCI        1388  CCTCAGC              CCTCAGC(-5/-2)
             1393  GCTGAGG             
BsaAI         256  CACGTG               [CT]AC^GT[GA]
              777  CACGTG              
BsrFI         951  GCCGGC               [GA]^CCGG[CT]
              994  ACCGGC              
BstEII        411  GGTCACC              G^GT[ACGT]ACC
             1311  GGTCACC             
BstPI         411  GGTCACC              G^GT[ACGT]ACC
             1311  GGTCACC             
Cfr10I        951  GCCGGC               [GA]^CCGG[CT]
              994  ACCGGC              
EaeI          266  TGGCCA               [CT]^GGCC[GA]
              573  TGGCCA              
EarI          165  GAAGAG               CTCTTC(1/4)
             1297  GAAGAG              
EcoO65I       411  GGTCACC              G^GT[ACGT]ACC
             1311  GGTCACC             
HgaI          376  GACGC                GACGC(5/10)
             1198  GACGC               
Ksp632I       165  GAAGAG               CTCTTC(1/4)
             1297  GAAGAG              
MluNI         266  TGGCCA               TGG^CCA
              573  TGGCCA              
MscI          266  TGGCCA               TGG^CCA
              573  TGGCCA              
MslI          330  CACCATCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1275  CACTGACGTG          
PleI          550  GACTC                GAGTC(4/5)
              568  GACTC               
PmaCI         256  CACGTG               CAC^GTG
              777  CACGTG              
PmlI          256  CACGTG               CAC^GTG
              777  CACGTG              
SacI          766  GAGCTC               GAGCT^C
              808  GAGCTC              
TfiI          170  GAATC                G^A[AT]TC
              535  GATTC               
Tsp509I      1325  AATT                 ^AATT
             1334  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   71 enzymes) #
#########################################

AccII       AciI        AluI        ApaI        AspHI       
AvaII       BanII       BbvI        BcnI        BmyI        
BpmI        BsaJI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       EcoO109I    
EcoRII      FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        MaeII       MaeIII      MboI        
MboII       MnlI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PpuMI       PstI        PvuII       
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
StuI        TaqI        TseI        Tsp45I      TspRI       
TthHB8I     
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