Restriction map for pF1KB8726
##################################
# NO CUT ENZYMES (  123 enzymes) #
##################################

Acc65I      AccI        AclI        AcsI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AviII       
AvrII       BalI        BamHI       BbeI        BbrPI       
BcgI        BclI        BfaI        BfrI        BglI        
BlnI        BlpI        Bpu1102I    BsaAI       BsgI        
BsiWI       BsmBI       BsmI        BspHI       BspLU11I    
BsrBI       BssHII      Bst1107I    BstBI       BstEII      
BstPI       BstZ17I     CelII       CpoI        DraI        
DrdI        EagI        Eam1105I    EarI        EclXI       
Eco52I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HpaI        HphI        
KasI        KpnI        Ksp632I     KspI        MaeI        
MfeI        MluI        MluNI       MscI        MseI        
MunI        NaeI        NarI        NcoI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    SspI        StuI        SwaI        Tru9I       
Tsp45I      Tsp509I     TspRI       XbaI        XcmI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AatII         766  GACGTC               GACGT^C
AccIII       1045  TCCGGA               T^CCGGA
AocI           18  CCTAAGG              CC^T[ACGT]AGG
Aor51HI       476  AGCGCT               AGC^GCT
AspHI         688  GAGCAC               G[AT]GC[AT]^C
AspI          391  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanI          975  GGTGCC               G^G[CT][GA]CC
BanII         317  GAGCCC               G[GA]GC[CT]^C
BbsI          840  GTCTTC               GAAGAC(2/6)
BciVI         677  GTATCC               GTATCC(6/5)
BcnI          402  CCGGG                CC^[GC]GG
BglII         230  AGATCT               A^GATCT
BpuAI         840  GTCTTC               GAAGAC(2/6)
BsaWI        1045  TCCGGA               [AT]^CCGG[AT]
BseAI        1045  TCCGGA               T^CCGGA
BsiEI         129  CGACCG               CG[GA][CT]^CG
BsiHKAI       688  GAGCAC               G[AT]GC[AT]^C
Bsp1407I      617  TGTACA               T^GTACA
BspDI         787  ATCGAT               AT^CGAT
BspEI        1045  TCCGGA               T^CCGGA
BspMI         724  ACCTGC               ACCTGC(4/8)
BsrDI         494  GCAATG               GCAATG(2/0)
BsrGI         617  TGTACA               T^GTACA
BsrI          894  ACTGG                ACTGG(1/-1)
BssSI         807  CTCGTG               CACGAG(-5/-1)
BstXI         936  CCACAAGCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I         18  CCTAAGG              CC^T[ACGT]AGG
ClaI          787  ATCGAT               AT^CGAT
DraIII        691  CACCGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          204  CCACGG               C^C[GA][CT]GG
EaeI         1010  TGGCCG               [CT]^GGCC[GA]
Eco47III      476  AGCGCT               AGC^GCT
Eco81I         18  CCTAAGG              CC^T[ACGT]AGG
EcoT14I       581  CCAAGG               C^C[AT][AT]GG
HgaI          278  GCGTC                GACGC(5/10)
HincII        199  GTCAAC               GT[CT]^[GA]AC
HindII        199  GTCAAC               GT[CT]^[GA]AC
HindIII       940  AAGCTT               A^AGCTT
MaeIII         32  GTTAC                ^GT[ACGT]AC
MroI         1045  TCCGGA               T^CCGGA
MspA1I        775  CAGCGG               C[AC]G^C[GT]G
NciI          402  CCGGG                CC^[GC]GG
NdeI          539  CATATG               CA^TATG
NspI          752  GCATGC               [GA]CATG^[CT]
PaeR7I        295  CTCGAG               C^TCGAG
PflFI         391  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         381  CCACGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          652  GACTC                GAGTC(4/5)
ScaI          374  AGTACT               AGT^ACT
SphI          752  GCATGC               GCATG^C
SspBI         617  TGTACA               T^GTACA
StyI          581  CCAAGG               C^C[AT][AT]GG
TfiI          164  GATTC                G^A[AT]TC
Tth111I       391  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        381  CCACGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          295  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AccII         707  CGCG                 CG^CG
              880  CGCG                
AcyI          277  GGCGTC               G[GA]^CG[CT]C
              766  GACGTC              
AvaII         405  GGACC                G^G[AT]CC
              738  GGACC               
BpmI         1020  CTCCAG               CTGGAG(16/14)
              597  CTGGAG              
BsaBI         180  GATGGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              255  GATCTACATC          
BsaHI         277  GGCGTC               G[GA]^CG[CT]C
              766  GACGTC              
BsaI          238  GAGACC               GGTCTC(1/5)
             1015  GAGACC              
BsmAI         238  GAGAC                GTCTC(1/5)
             1015  GAGAC               
BsmFI         404  GGGAC                GGGAC(10/14)
              488  GGGAC               
BsrFI         926  ACCGGC               [GA]^CCGG[CT]
              962  ACCGGC              
BstAPI        535  GCAGCATATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              960  GCACCGGCTGC         
BstUI         707  CGCG                 CG^CG
              880  CGCG                
Cfr10I        926  ACCGGC               [GA]^CCGG[CT]
              962  ACCGGC              
DraII         404  GGGACCT              [GA]G^G[ACGT]CC[CT]
              737  AGGACCT             
EcoO109I      404  GGGACCT              [GA]G^G[ACGT]CC[CT]
              737  AGGACCT             
HaeII          95  AGCGCC               [GA]GCGC^[CT]
              476  AGCGCT              
Hin1I         277  GGCGTC               G[GA]^CG[CT]C
              766  GACGTC              
HinfI         164  GATTC                G^A[ACGT]TC
              652  GACTC               
MamI          180  GATGGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              255  GATCTACATC          
MslI          261  CATCATCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              784  CACATCGATG          
MvnI          707  CGCG                 CG^CG
              880  CGCG                
PpuMI         404  GGGACCT              [GA]G^G[AT]CC[CT]
              737  AGGACCT             


#########################################
# 3 OR MORE CUT ENZYMES (   57 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AvaI        
BbvCI       BbvI        BmyI        BsaJI       BseRI       
BsiYI       BslI        BsoBI       Bsp1286I    BssKI       
BstF5I      BstNI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       EcoNI       EcoRII      
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HpaII       ItaI        MaeII       
MboI        MboII       MflI        MnlI        MspI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
PstI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        TaqI        TseI        
TthHB8I     XhoII       
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