Restriction map for pF1KB8726
 (Linear) (Six-base) MAPSORT of: KIBB8726.seq  Check: 6692  from: 1  to: 1074



KIBB8726.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:07  ..

AatII G_ACGT'C

Cuts at:      0     770    1074 
   Size:        770     304
 
AfeI AGC'GCT

Cuts at:      0     478    1074 
   Size:        478     596
 
AvaI C'yCGr_G

Cuts at:      0     173     295     550     979    1074 
   Size:        173     122     255     429      95

  Fragments arranged by size: 

                 429     255     173     122      95
 
BanI G'GyrC_C

Cuts at:      0     975    1074 
   Size:        975      99
 
BanII G_rGCy'C

Cuts at:      0     321    1074 
   Size:        321     753
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     833    1074 
   Size:        833     241
 
BbvCI CC'TCA_GC

Cuts at:      0     511     799    1052    1074 
   Size:        511     288     253      22

  Fragments arranged by size: 

                 511     288     253      22
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     688    1074 
   Size:        688     386
 
BglII A'GATC_T

Cuts at:      0     230    1074 
   Size:        230     844
 
Bme1580I G_kGCm'C

Cuts at:      0     980    1074 
   Size:        980      94
 
BmgBI CAC'GTC

Cuts at:      0     333     839    1074 
   Size:        333     506     235

  Fragments arranged by size: 

                 506     333     235
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     618    1005    1074 
   Size:        618     387      69

  Fragments arranged by size: 

                 618     387      69
 
Bpu10I CC'TnA_GC

Cuts at:      0     511     799    1052    1074 
   Size:        511     288     253      22

  Fragments arranged by size: 

                 511     288     253      22
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      85     394     933    1074 
   Size:         85     309     539     141

  Fragments arranged by size: 

                 539     309     141      85
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     232    1009    1074 
   Size:        232     777      65

  Fragments arranged by size: 

                 777     232      65
 
BsaBI GATnn'nnATC

Cuts at:      0     184     259    1074 
   Size:        184      75     815

  Fragments arranged by size: 

                 815     184      75
 
BsaHI Gr'CG_yC

Cuts at:      0     278     767    1074 
   Size:        278     489     307

  Fragments arranged by size: 

                 489     307     278
 
BsaWI w'CCGG_w

Cuts at:      0    1045    1074 
   Size:       1045      29
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     193     223     859     889    1040    1070    1074 
   Size:        193      30     636      30     151      30       4

  Fragments arranged by size: 

                 636     193     151      30      30      30       4
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     283     351     418    1074 
   Size:        283      68      67     656

  Fragments arranged by size: 

                 656     283      68      67
 
BseYI C'CCAG_C

Cuts at:      0     956    1074 
   Size:        956     118
 
BsiEI CG_ry'CG

Cuts at:      0     132    1074 
   Size:        132     942
 
BsiHKAI G_wGCw'C

Cuts at:      0     692    1074 
   Size:        692     382
 
Bsp1286I G_dGCh'C

Cuts at:      0     321     692     980    1074 
   Size:        321     371     288      94

  Fragments arranged by size: 

                 371     321     288      94
 
BspEI T'CCGG_A

Cuts at:      0    1045    1074 
   Size:       1045      29
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     733    1074 
   Size:        733     341
 
BsrDI GCAATG_nn'

Cuts at:      0     501    1074 
   Size:        501     573
 
BsrFI r'CCGG_y

Cuts at:      0     926     962    1074 
   Size:        926      36     112

  Fragments arranged by size: 

                 926     112      36
 
BsrGI T'GTAC_A

Cuts at:      0     617    1074 
   Size:        617     457
 
BssSI C'ACGA_G

Cuts at:      0     807    1074 
   Size:        807     267
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     541     966    1074 
   Size:        541     425     108

  Fragments arranged by size: 

                 541     425     108
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     943    1074 
   Size:        943     131
 
BstYI r'GATC_y

Cuts at:      0     230     254     794    1074 
   Size:        230      24     540     280

  Fragments arranged by size: 

                 540     280     230      24
 
Bsu36I CC'TnA_GG

Cuts at:      0      19    1074 
   Size:         19    1055
 
BtgI C'CryG_G

Cuts at:      0     204    1074 
   Size:        204     870
 
ClaI AT'CG_AT

Cuts at:      0     788    1074 
   Size:        788     286
 
DraIII CAC_nnn'GTG

Cuts at:      0     696    1074 
   Size:        696     378
 
EaeI y'GGCC_r

Cuts at:      0    1010    1074 
   Size:       1010      64
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     354    1074 
   Size:        354     720
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     618    1005    1074 
   Size:        618     387      69

  Fragments arranged by size: 

                 618     387      69
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     325     433     855    1074 
   Size:        325     108     422     219

  Fragments arranged by size: 

                 422     325     219     108
 
EcoO109I rG'GnC_Cy

Cuts at:      0     405     738    1074 
   Size:        405     333     336

  Fragments arranged by size: 

                 405     336     333
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     927    1074 
   Size:        927     147
 
HaeII r_GCGC'y

Cuts at:      0      99     480    1074 
   Size:         99     381     594

  Fragments arranged by size: 

                 594     381      99
 
HincII GTy'rAC

Cuts at:      0     201    1074 
   Size:        201     873
 
HindIII A'AGCT_T

Cuts at:      0     940    1074 
   Size:        940     134
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     375     680    1074 
   Size:        375     305     394

  Fragments arranged by size: 

                 394     375     305
 
MslI CAynn'nnrTG

Cuts at:      0     265     788    1074 
   Size:        265     523     286

  Fragments arranged by size: 

                 523     286     265
 
MspA1I CmG'CkG

Cuts at:      0     777    1074 
   Size:        777     297
 
NdeI CA'TA_TG

Cuts at:      0     540    1074 
   Size:        540     534
 
NspI r_CATG'y

Cuts at:      0     756    1074 
   Size:        756     318
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     387    1074 
   Size:        387     687
 
PpuMI rG'GwC_Cy

Cuts at:      0     405     738    1074 
   Size:        405     333     336

  Fragments arranged by size: 

                 405     336     333
 
PspXI vC'TCGA_Gb

Cuts at:      0     295    1074 
   Size:        295     779
 
PstI C_TGCA'G

Cuts at:      0     563     761     818    1074 
   Size:        563     198      57     256

  Fragments arranged by size: 

                 563     256     198      57
 
ScaI AGT'ACT

Cuts at:      0     376    1074 
   Size:        376     698
 
SfcI C'TryA_G

Cuts at:      0     559     757     814    1074 
   Size:        559     198      57     260

  Fragments arranged by size: 

                 559     260     198      57
 
SmlI C'TyrA_G

Cuts at:      0     100     295     409     912    1074 
   Size:        100     195     114     503     162

  Fragments arranged by size: 

                 503     195     162     114     100
 
SphI G_CATG'C

Cuts at:      0     756    1074 
   Size:        756     318
 
StyI C'CwwG_G

Cuts at:      0     581    1074 
   Size:        581     493
 
TatI w'GTAC_w

Cuts at:      0     374     617    1074 
   Size:        374     243     457

  Fragments arranged by size: 

                 457     374     243
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     397     429    1040    1072    1074 
   Size:        397      32     611      32       2

  Fragments arranged by size: 

                 611     397      32      32       2
 
Tth111I GACn'n_nGTC

Cuts at:      0     394    1074 
   Size:        394     680
 
XhoI C'TCGA_G

Cuts at:      0     295    1074 
   Size:        295     779
 
ZraI GAC'GTC

Cuts at:      0     768    1074 
   Size:        768     306
 

 Enzymes that do cut:

    AatII     AfeI     AvaI     BanI    BanII     BbsI    BbvCI    BciVI
    BglII Bme1580I    BmgBI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI Bsp1286I
    BspEI    BspMI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstXI
    BstYI   Bsu36I     BtgI     ClaI   DraIII     EaeI     EciI  Eco57MI
    EcoNI EcoO109I     FalI    HaeII   HincII  HindIII     MmeI     MslI
   MspA1I     NdeI     NspI    PflMI    PpuMI    PspXI     PstI     ScaI
     SfcI     SmlI     SphI     StyI     TatI     TstI  Tth111I     XhoI
     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI     BcgI
     BclI    BfrBI     BglI     BlpI     BmrI     BmtI     BplI    BsaAI
     BsgI    BsiWI     BsmI    BsmBI    BspHI    BsrBI   BssHII    BstBI
   BstEII  BstZ17I    BtgZI     BtsI    CspCI     DraI     DrdI     EagI
     EarI  EcoICRI    EcoRI    EcoRV     FseI     FspI    FspAI    Hin4I
     HpaI     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI
     NcoI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SrfI     SspI     StuI     SwaI    TaqII     XbaI
     XcmI     XmaI     XmnI
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