Restriction map for pF1KB8722
 (Linear) (Six-base) MAPSORT of: KIBB8722.seq  Check: 5509  from: 1  to: 1560



KIBB8722.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:07  ..

AccI GT'mk_AC

Cuts at:      0     149     374    1560 
   Size:        149     225    1186

  Fragments arranged by size: 

                1186     225     149
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1533    1560 
   Size:       1533      27
 
AfeI AGC'GCT

Cuts at:      0     741    1560 
   Size:        741     819
 
AflII C'TTAA_G

Cuts at:      0     208    1560 
   Size:        208    1352
 
AhdI GACnn_n'nnGTC

Cuts at:      0     255    1560 
   Size:        255    1305
 
AleI CACnn'nnGTG

Cuts at:      0    1294    1560 
   Size:       1294     266
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1556    1560 
   Size:       1556       4
 
ApaI G_GGCC'C

Cuts at:      0    1118    1417    1560 
   Size:       1118     299     143

  Fragments arranged by size: 

                1118     299     143
 
ApoI r'AATT_y

Cuts at:      0      96    1560 
   Size:         96    1464
 
AvaI C'yCGr_G

Cuts at:      0     611    1560 
   Size:        611     949
 
BanI G'GyrC_C

Cuts at:      0     952    1560 
   Size:        952     608
 
BanII G_rGCy'C

Cuts at:      0     446     632    1118    1417    1490    1560 
   Size:        446     186     486     299      73      70

  Fragments arranged by size: 

                 486     446     299     186      73      70
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1354    1560 
   Size:       1354     206
 
BbvCI CC'TCA_GC

Cuts at:      0    1096    1560 
   Size:       1096     464
 
BclI T'GATC_A

Cuts at:      0     263    1560 
   Size:        263    1297
 
BglI GCCn_nnn'nGGC

Cuts at:      0     385    1560 
   Size:        385    1175
 
BglII A'GATC_T

Cuts at:      0      81    1560 
   Size:         81    1479
 
Bme1580I G_kGCm'C

Cuts at:      0    1118    1417    1560 
   Size:       1118     299     143

  Fragments arranged by size: 

                1118     299     143
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     148     304    1044    1523    1560 
   Size:        148     156     740     479      37

  Fragments arranged by size: 

                 740     479     156     148      37
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     465    1560 
   Size:        465    1095
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     457     898    1560 
   Size:        457     441     662

  Fragments arranged by size: 

                 662     457     441
 
Bpu10I CC'TnA_GC

Cuts at:      0    1096    1560 
   Size:       1096     464
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1342    1560 
   Size:       1342     218
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     601     610     766    1136    1366    1560 
   Size:        601       9     156     370     230     194

  Fragments arranged by size: 

                 601     370     230     194     156       9
 
BsaAI yAC'GTr

Cuts at:      0     843    1560 
   Size:        843     717
 
BsaWI w'CCGG_w

Cuts at:      0     974    1560 
   Size:        974     586
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1537    1560 
   Size:       1537      23
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1285    1330    1560 
   Size:       1285      45     230

  Fragments arranged by size: 

                1285     230      45
 
BseYI C'CCAG_C

Cuts at:      0     229    1455    1560 
   Size:        229    1226     105

  Fragments arranged by size: 

                1226     229     105
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     144     835     868    1560 
   Size:        144     691      33     692

  Fragments arranged by size: 

                 692     691     144      33
 
BsiEI CG_ry'CG

Cuts at:      0     365    1560 
   Size:        365    1195
 
BsiHKAI G_wGCw'C

Cuts at:      0     632    1560 
   Size:        632     928
 
Bsp1286I G_dGCh'C

Cuts at:      0     446     632    1118    1417    1490    1560 
   Size:        446     186     486     299      73      70

  Fragments arranged by size: 

                 486     446     299     186      73      70
 
BspEI T'CCGG_A

Cuts at:      0     974    1560 
   Size:        974     586
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     437     916    1560 
   Size:        437     479     644

  Fragments arranged by size: 

                 644     479     437
 
BsrBI CCG'CTC

Cuts at:      0    1344    1560 
   Size:       1344     216
 
BsrDI GCAATG_nn'

Cuts at:      0     240    1560 
   Size:        240    1320
 
BsrFI r'CCGG_y

Cuts at:      0     364    1560 
   Size:        364    1196
 
BsrGI T'GTAC_A

Cuts at:      0      32    1560 
   Size:         32    1528
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     489     762    1560 
   Size:        489     273     798

  Fragments arranged by size: 

                 798     489     273
 
BstYI r'GATC_y

Cuts at:      0      81     967    1274    1560 
   Size:         81     886     307     286

  Fragments arranged by size: 

                 886     307     286      81
 
Bsu36I CC'TnA_GG

Cuts at:      0    1265    1560 
   Size:       1265     295
 
BtgI C'CryG_G

Cuts at:      0     202    1086    1226    1560 
   Size:        202     884     140     334

  Fragments arranged by size: 

                 884     334     202     140
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     775    1560 
   Size:        775     785
 
BtsI GCAGTG_nn'

Cuts at:      0     120     303     395     885    1560 
   Size:        120     183      92     490     675

  Fragments arranged by size: 

                 675     490     183     120      92
 
DraIII CAC_nnn'GTG

Cuts at:      0     348    1560 
   Size:        348    1212
 
EaeI y'GGCC_r

Cuts at:      0    1103    1560 
   Size:       1103     457
 
EarI CTCTTCn'nnn_

Cuts at:      0     551    1378    1478    1560 
   Size:        551     827     100      82

  Fragments arranged by size: 

                 827     551     100      82
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1417    1560 
   Size:       1417     143
 
EcoICRI GAG'CTC

Cuts at:      0     630    1560 
   Size:        630     930
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     457     898    1533    1560 
   Size:        457     441     635      27

  Fragments arranged by size: 

                 635     457     441      27
 
EcoO109I rG'GnC_Cy

Cuts at:      0     257     426     477     573     678    1413    1414    1560 
   Size:        257     169      51      96     105     735       1     146

  Fragments arranged by size: 

                 735     257     169     146     105      96      51       1
            
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     291    1560 
   Size:        291    1269
 
FspI TGC'GCA

Cuts at:      0    1337    1560 
   Size:       1337     223
 
HaeII r_GCGC'y

Cuts at:      0     743    1560 
   Size:        743     817
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     241     273    1537    1560 
   Size:        241      32    1264      23

  Fragments arranged by size: 

                1264     241      32      23
 
MscI TGG'CCA

Cuts at:      0    1105    1560 
   Size:       1105     455
 
MslI CAynn'nnrTG

Cuts at:      0      62     760    1294    1560 
   Size:         62     698     534     266

  Fragments arranged by size: 

                 698     534     266      62
 
MspA1I CmG'CkG

Cuts at:      0     790    1088    1560 
   Size:        790     298     472

  Fragments arranged by size: 

                 790     472     298
 
NcoI C'CATG_G

Cuts at:      0     202    1226    1560 
   Size:        202    1024     334

  Fragments arranged by size: 

                1024     334     202
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     348    1437    1560 
   Size:        348    1089     123

  Fragments arranged by size: 

                1089     348     123
 
PfoI T'CCnGG_A

Cuts at:      0     422     473     767    1560 
   Size:        422      51     294     793

  Fragments arranged by size: 

                 793     422     294      51
 
PpuMI rG'GwC_Cy

Cuts at:      0     257     426     477     573    1560 
   Size:        257     169      51      96     987

  Fragments arranged by size: 

                 987     257     169      96      51
 
PspOMI G'GGCC_C

Cuts at:      0    1114    1413    1560 
   Size:       1114     299     147

  Fragments arranged by size: 

                1114     299     147
 
PstI C_TGCA'G

Cuts at:      0     127     140     626    1560 
   Size:        127      13     486     934

  Fragments arranged by size: 

                 934     486     127      13
 
PvuII CAG'CTG

Cuts at:      0     790    1560 
   Size:        790     770
 
SacI G_AGCT'C

Cuts at:      0     632    1560 
   Size:        632     928
 
SacII CC_GC'GG

Cuts at:      0    1089    1560 
   Size:       1089     471
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1378    1478    1560 
   Size:       1378     100      82

  Fragments arranged by size: 

                1378     100      82
 
SbfI CC_TGCA'GG

Cuts at:      0     626    1560 
   Size:        626     934
 
SfcI C'TryA_G

Cuts at:      0     123     136     622    1292    1560 
   Size:        123      13     486     670     268

  Fragments arranged by size: 

                 670     486     268     123      13
 
SmlI C'TyrA_G

Cuts at:      0     208    1357    1560 
   Size:        208    1149     203

  Fragments arranged by size: 

                1149     208     203
 
StyI C'CwwG_G

Cuts at:      0     202     990    1226    1560 
   Size:        202     788     236     334

  Fragments arranged by size: 

                 788     334     236     202
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     355     451    1560 
   Size:        355      96    1109

  Fragments arranged by size: 

                1109     355      96
 
TatI w'GTAC_w

Cuts at:      0      32    1445    1560 
   Size:         32    1413     115

  Fragments arranged by size: 

                1413     115      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1233    1560 
   Size:       1233     327
 
XmnI GAAnn'nnTTC

Cuts at:      0    1045    1560 
   Size:       1045     515
 

 Enzymes that do cut:

     AccI     AcuI     AfeI    AflII     AhdI     AleI    AlwNI     ApaI
     ApoI     AvaI     BanI    BanII     BbsI    BbvCI     BclI     BglI
    BglII Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
 Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI   DraIII     EaeI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I     FalI     FspI    HaeII    Hin4I
     MscI     MslI   MspA1I     NcoI    PflMI     PfoI    PpuMI   PspOMI
     PstI    PvuII     SacI    SacII     SapI     SbfI     SfcI     SmlI
     StyI    TaqII     TatI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI   AflIII     AgeI     AlfI     AloI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI
     BcgI    BciVI    BfrBI     BlpI    BmgBI     BmtI    BsaBI    BsaHI
    BsiWI     BsmI    BsmBI    BspHI   BssHII    BssSI   BstAPI    BstBI
   BstEII  BstZ17I     ClaI    CspCI     DraI     DrdI     EagI    EcoNI
    EcoRI    EcoRV     FseI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI
    RsrII     SalI    SanDI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TstI  Tth111I     XbaI     XhoI     XmaI     ZraI
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