Restriction map for pF1KB8721
##################################
# NO CUT ENZYMES (  102 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AcyI        AflII       AflIII      AgeI        AhdI        
Aor51HI     ApoI        AscI        Asp700I     Asp718I     
AspEI       AspI        AviII       AvrII       BamHI       
BbeI        BbrPI       BciVI       BclI        BfrI        
BlnI        BsaAI       BsaHI       BsaWI       BseAI       
BsiWI       BsmBI       BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       Bst1107I    BstBI       
BstZ17I     ClaI        CpoI        DraI        Eam1105I    
Eco47III    EcoRI       EcoRV       EcoT22I     FbaI        
FspI        HaeII       HgaI        Hin1I       HindIII     
HpaI        KasI        KpnI        KspI        MfeI        
MluI        MroI        MunI        NarI        NdeI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SapI        
SbfI        ScaI        SexAI       SfoI        SfuI        
SgrAI       SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspI        SwaI        Tth111I     XbaI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccII         618  CGCG                 CG^CG
AfaI         1100  GTAC                 GT^AC
AocI          228  CCTCAGG              CC^T[ACGT]AGG
AseI          868  ATTAAT               AT^TAAT
AsnI          868  ATTAAT               AT^TAAT
BanI          712  GGTGCC               G^G[CT][GA]CC
BbsI         1614  GAAGAC               GAAGAC(2/6)
BbvCI        1522  GCTGAGG              CCTCAGC(-5/-2)
BcgI          311  GCACAGCCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII        1592  AGATCT               A^GATCT
BpuAI        1614  GAAGAC               GAAGAC(2/6)
BsaBI         960  GATGGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI         1160  GTGCAG               GTGCAG(16/14)
BsiEI         162  CGGCCG               CG[GA][CT]^CG
Bsp1407I     1099  TGTACA               T^GTACA
BsrGI        1099  TGTACA               T^GTACA
BssHII        617  GCGCGC               G^CGCGC
BssSI        1540  CTCGTG               CACGAG(-5/-1)
BstAPI       1357  GCAGAGAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        180  GGTGACC              G^GT[ACGT]ACC
BstPI         180  GGTGACC              G^GT[ACGT]ACC
BstUI         618  CGCG                 CG^CG
Bsu36I        228  CCTCAGG              CC^T[ACGT]AGG
DraIII        207  CACGGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI            4  GACAGCACAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI          162  CGGCCG               C^GGCCG
EarI           79  GAAGAG               CTCTTC(1/4)
EclXI         162  CGGCCG               C^GGCCG
Eco52I        162  CGGCCG               C^GGCCG
Eco81I        228  CCTCAGG              CC^T[ACGT]AGG
EcoNI         975  CCTGAGTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       180  GGTGACC              G^GT[ACGT]ACC
FauI          387  CCCGC                CCCGC(4/6)
FseI          372  GGCCGGCC             GGCCGG^CC
HincII        895  GTCGAC               GT[CT]^[GA]AC
HindII        895  GTCGAC               GT[CT]^[GA]AC
Ksp632I        79  GAAGAG               CTCTTC(1/4)
MaeII        1385  ACGT                 A^CGT
MamI          960  GATGGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI          618  CGCG                 CG^CG
NcoI          527  CCATGG               C^CATGG
NheI          852  GCTAGC               G^CTAGC
PpuMI         425  AGGTCCT              [GA]G^G[AT]CC[CT]
PshBI         868  ATTAAT               AT^TAAT
RsaI         1100  GTAC                 GT^AC
SalI          895  GTCGAC               G^TCGAC
SspBI        1099  TGTACA               T^GTACA
StuI          401  AGGCCT               AGG^CCT
XcmI          452  CCATCCAATCTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AccI          895  GTCGAC               GT^[AC][GT]AC
             1174  GTCTAC              
Alw44I        204  GTGCAC               G^TGCAC
             1408  GTGCAC              
AlwI          478  GATCC                GGATC(4/5)
             1213  GATCC               
ApaLI         204  GTGCAC               G^TGCAC
             1408  GTGCAC              
BlpI           99  GCTGAGC              GC^T[ACGT]AGC
             1370  GCTTAGC             
Bpu1102I       99  GCTGAGC              GC^T[ACGT]AGC
             1370  GCTTAGC             
BsaI          904  GAGACC               GGTCTC(1/5)
              953  GGTCTC              
BsmI         1362  GAATGC               GAATGC(1/-1)
             1309  GCATTC              
BspMI          95  ACCTGC               ACCTGC(4/8)
             1110  ACCTGC              
BsrFI         164  GCCGGC               [GA]^CCGG[CT]
              373  GCCGGC              
CelII          99  GCTGAGC              GC^T[ACGT]AGC
             1370  GCTTAGC             
CfoI          617  GCGC                 GCG^C
              619  GCGC                
Cfr10I        164  GCCGGC               [GA]^CCGG[CT]
              373  GCCGGC              
HhaI          617  GCGC                 GCG^C
              619  GCGC                
HinP1I        617  GCGC                 G^CGC
              619  GCGC                
HphI          180  GGTGA                GGTGA(8/7)
              939  GGTGA               
MaeIII        181  GTGAC                ^GT[ACGT]AC
             1543  GTGAC               
MseI          706  TTAA                 T^TAA
              869  TTAA                
NaeI          164  GCCGGC               GCC^GGC
              373  GCCGGC              
NgoMIV        164  GCCGGC               G^CCGGC
              373  GCCGGC              
PleI         1606  GACTC                GAGTC(4/5)
               72  GAGTC               
PvuII         361  CAGCTG               CAG^CTG
              857  CAGCTG              
SfaNI        1189  GATGC                GCATC(5/9)
              932  GCATC               
SfiI          154  GGCCCCCACGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             1295  GGCCCAGTGGGCC       
SmaI          625  CCCGGG               CCC^GGG
              657  CCCGGG              
TaqI          896  TCGA                 T^CGA
             1512  TCGA                
Tru9I         706  TTAA                 T^TAA
              869  TTAA                
Tsp45I        181  GTGAC                ^GT[GC]AC
             1543  GTGAC               
TthHB8I       896  TCGA                 T^CGA
             1512  TCGA                
XmaCI         625  CCCGGG               C^CCGGG
              657  CCCGGG              
XmaI          625  CCCGGG               C^CCGGG
              657  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   76 enzymes) #
#########################################

AciI        AluI        AlwNI       ApaI        AspHI       
AvaI        AvaII       BalI        BanII       BbvI        
BcnI        BfaI        BglI        BmyI        BpmI        
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstXI       BstYI       
Cac8I       Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EcoO109I    EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       ItaI        MaeI        MboI        MboII       
MflI        MluNI       MnlI        MscI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PflMI       PstI        
Sau3AI      Sau96I      ScrFI       SfcI        StyI        
TfiI        TseI        Tsp509I     TspRI       Van91I      
XhoII       
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