Restriction map for
pF1KB8719
(Linear) (Six-base) MAPSORT of: KIBB8719.seq Check: 2952 from: 1 to: 1428
KIBB8719.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 717 773 1428
Size: 717 56 655
Fragments arranged by size:
717 655 56
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 750 1090 1428
Size: 750 340 338
Fragments arranged by size:
750 340 338
AleI CACnn'nnGTG
Cuts at: 0 352 1428
Size: 352 1076
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1415 1428
Size: 1415 13
AlwNI CAG_nnn'CTG
Cuts at: 0 349 1428
Size: 349 1079
ApaI G_GGCC'C
Cuts at: 0 844 1239 1428
Size: 844 395 189
Fragments arranged by size:
844 395 189
ApoI r'AATT_y
Cuts at: 0 663 1428
Size: 663 765
AvaI C'yCGr_G
Cuts at: 0 57 284 878 1428
Size: 57 227 594 550
Fragments arranged by size:
594 550 227 57
BanI G'GyrC_C
Cuts at: 0 785 1326 1428
Size: 785 541 102
Fragments arranged by size:
785 541 102
BanII G_rGCy'C
Cuts at: 0 293 844 1174 1239 1428
Size: 293 551 330 65 189
Fragments arranged by size:
551 330 293 189 65
BbeI G_GCGC'C
Cuts at: 0 1330 1428
Size: 1330 98
BbsI GAAGACnn'nnnn_
Cuts at: 0 441 1428
Size: 441 987
BbvCI CC'TCA_GC
Cuts at: 0 551 1428
Size: 551 877
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 5 39 1072 1106 1428
Size: 5 34 1033 34 322
Fragments arranged by size:
1033 322 34 34 5
BglI GCCn_nnn'nGGC
Cuts at: 0 848 1084 1243 1428
Size: 848 236 159 185
Fragments arranged by size:
848 236 185 159
Bme1580I G_kGCm'C
Cuts at: 0 528 844 1239 1428
Size: 528 316 395 189
Fragments arranged by size:
528 395 316 189
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 169 296 371 412 428 778 1060
Size: 124 45 127 75 41 16 350 282
Cuts at: 1060 1428
Size: 368
Fragments arranged by size:
368 350 282 127 124 75 45 41
16
Bpu10I CC'TnA_GC
Cuts at: 0 454 551 778 1168 1428
Size: 454 97 227 390 260
Fragments arranged by size:
454 390 260 227 97
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 996 1267 1428
Size: 996 271 161
Fragments arranged by size:
996 271 161
BsaI GGTCTCn'nnnn_
Cuts at: 0 634 1428
Size: 634 794
BsaAI yAC'GTr
Cuts at: 0 1005 1428
Size: 1005 423
BsaBI GATnn'nnATC
Cuts at: 0 250 1428
Size: 250 1178
BsaHI Gr'CG_yC
Cuts at: 0 7 1327 1428
Size: 7 1320 101
Fragments arranged by size:
1320 101 7
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 466 496 694 724 1354 1384 1428
Size: 466 30 198 30 630 30 44
Fragments arranged by size:
630 466 198 44 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 229 427 430 433 715 1428
Size: 229 198 3 3 282 713
Fragments arranged by size:
713 282 229 198 3 3
BseYI C'CCAG_C
Cuts at: 0 82 436 818 1023 1062 1173 1263 1428
Size: 82 354 382 205 39 111 90 165
Fragments arranged by size:
382 354 205 165 111 90 82 39
BsiEI CG_ry'CG
Cuts at: 0 384 1428
Size: 384 1044
BsiHKAI G_wGCw'C
Cuts at: 0 608 1428
Size: 608 820
Bsp1286I G_dGCh'C
Cuts at: 0 293 528 608 844 1174 1239 1428
Size: 293 235 80 236 330 65 189
Fragments arranged by size:
330 293 236 235 189 80 65
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 717 773 1394 1428
Size: 717 56 621 34
Fragments arranged by size:
717 621 56 34
BsrBI CCG'CTC
Cuts at: 0 1059 1428
Size: 1059 369
BsrFI r'CCGG_y
Cuts at: 0 1323 1428
Size: 1323 105
BssSI C'ACGA_G
Cuts at: 0 465 1428
Size: 465 963
BstYI r'GATC_y
Cuts at: 0 1034 1428
Size: 1034 394
Bsu36I CC'TnA_GG
Cuts at: 0 332 754 1428
Size: 332 422 674
Fragments arranged by size:
674 422 332
BtgI C'CryG_G
Cuts at: 0 378 1428
Size: 378 1050
EaeI y'GGCC_r
Cuts at: 0 381 506 1076 1145 1214 1292 1428
Size: 381 125 570 69 69 78 136
Fragments arranged by size:
570 381 136 125 78 69 69
EagI C'GGCC_G
Cuts at: 0 381 1428
Size: 381 1047
EarI CTCTTCn'nnn_
Cuts at: 0 116 551 562 1428
Size: 116 435 11 866
Fragments arranged by size:
866 435 116 11
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 169 296 371 412 428 750 778
Size: 124 45 127 75 41 16 322 28
Cuts at: 778 1060 1090 1428
Size: 282 30 338
Fragments arranged by size:
338 322 282 127 124 75 45 41
30 28 16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 115 1428
Size: 115 1313
EcoO109I rG'GnC_Cy
Cuts at: 0 53 108 135 374 759 841 1236 1362
Size: 53 55 27 239 385 82 395 126
Cuts at: 1362 1428
Size: 66
Fragments arranged by size:
395 385 239 126 82 66 55 53
27
EcoRI G'AATT_C
Cuts at: 0 663 1428
Size: 663 765
HaeII r_GCGC'y
Cuts at: 0 1200 1330 1428
Size: 1200 130 98
Fragments arranged by size:
1200 130 98
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 466 498 1125 1157 1354 1386 1428
Size: 466 32 627 32 197 32 42
Fragments arranged by size:
627 466 197 42 32 32 32
HindIII A'AGCT_T
Cuts at: 0 1412 1428
Size: 1412 16
KasI G'GCGC_C
Cuts at: 0 1326 1428
Size: 1326 102
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 971 1428
Size: 971 457
MscI TGG'CCA
Cuts at: 0 508 1078 1147 1428
Size: 508 570 69 281
Fragments arranged by size:
570 508 281 69
MslI CAynn'nnrTG
Cuts at: 0 352 502 1428
Size: 352 150 926
Fragments arranged by size:
926 352 150
MspA1I CmG'CkG
Cuts at: 0 105 380 432 708 1090 1428
Size: 105 275 52 276 382 338
Fragments arranged by size:
382 338 276 275 105 52
NarI GG'CG_CC
Cuts at: 0 1327 1428
Size: 1327 101
NspI r_CATG'y
Cuts at: 0 343 1428
Size: 343 1085
PfoI T'CCnGG_A
Cuts at: 0 110 488 1037 1428
Size: 110 378 549 391
Fragments arranged by size:
549 391 378 110
PpuMI rG'GwC_Cy
Cuts at: 0 108 759 1362 1428
Size: 108 651 603 66
Fragments arranged by size:
651 603 108 66
PspOMI G'GGCC_C
Cuts at: 0 840 1235 1428
Size: 840 395 193
Fragments arranged by size:
840 395 193
PspXI vC'TCGA_Gb
Cuts at: 0 878 1428
Size: 878 550
PstI C_TGCA'G
Cuts at: 0 1307 1394 1428
Size: 1307 87 34
Fragments arranged by size:
1307 87 34
PvuII CAG'CTG
Cuts at: 0 708 1428
Size: 708 720
SacII CC_GC'GG
Cuts at: 0 381 1428
Size: 381 1047
SanDI GG'GwC_CC
Cuts at: 0 759 1428
Size: 759 669
SfcI C'TryA_G
Cuts at: 0 1192 1303 1390 1428
Size: 1192 111 87 38
Fragments arranged by size:
1192 111 87 38
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1084 1243 1428
Size: 1084 159 185
Fragments arranged by size:
1084 185 159
SfoI GGC'GCC
Cuts at: 0 1328 1428
Size: 1328 100
SgrAI Cr'CCGG_yG
Cuts at: 0 1323 1428
Size: 1323 105
SmaI CCC'GGG
Cuts at: 0 286 1428
Size: 286 1142
SmlI C'TyrA_G
Cuts at: 0 878 975 1282 1428
Size: 878 97 307 146
Fragments arranged by size:
878 307 146 97
SphI G_CATG'C
Cuts at: 0 343 1428
Size: 343 1085
StuI AGG'CCT
Cuts at: 0 389 1166 1428
Size: 389 777 262
Fragments arranged by size:
777 389 262
StyI C'CwwG_G
Cuts at: 0 269 509 1428
Size: 269 240 919
Fragments arranged by size:
919 269 240
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 874 1291 1428
Size: 874 417 137
Fragments arranged by size:
874 417 137
TatI w'GTAC_w
Cuts at: 0 650 674 1223 1428
Size: 650 24 549 205
Fragments arranged by size:
650 549 205 24
XhoI C'TCGA_G
Cuts at: 0 878 1428
Size: 878 550
XmaI C'CCGG_G
Cuts at: 0 284 1428
Size: 284 1144
Enzymes that do cut:
AarI AcuI AleI AlfI AlwNI ApaI ApoI AvaI
BanI BanII BbeI BbsI BbvCI BcgI BglI Bme1580I
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI
BssSI BstYI Bsu36I BtgI EaeI EagI EarI Eco57MI
EcoNI EcoO109I EcoRI HaeII Hin4I HindIII KasI MmeI
MscI MslI MspA1I NarI NspI PfoI PpuMI PspOMI
PspXI PstI PvuII SacII SanDI SfcI SfiI SfoI
SgrAI SmaI SmlI SphI StuI StyI TaqII TatI
XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BciVI BclI BfrBI BglII BlpI BmgBI BmrI
BmtI BplI BsaWI BsgI BsiWI BsmI BsmBI BspEI
BspHI BsrDI BsrGI BssHII BstAPI BstBI BstEII BstXI
BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EciI EcoICRI EcoRV FalI FseI FspI FspAI HincII
HpaI KpnI MfeI MluI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PsrI PvuI RsrII
SacI SalI SapI SbfI ScaI SexAI SgfI SnaBI
SpeI SrfI SspI SwaI TstI Tth111I XbaI XcmI
XmnI ZraI