Restriction map for pF1KB8716
##################################
# NO CUT ENZYMES (  115 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
AocI        Aor51HI     ApoI        AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AviII       
AvrII       BbvCI       BcgI        BciVI       BclI        
BfaI        BfrI        BglI        BglII       BlnI        
BsaI        BsaWI       BseAI       BsiWI       BsmAI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspLU11I    BsrDI       BsrGI       BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstZ17I     Bsu36I      ClaI        CpoI        DraI        
Eam1105I    Eco47III    Eco81I      EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HindIII     HpaI        KpnI        MaeI        MfeI        
MluI        MroI        MseI        MunI        NcoI        
NdeI        NheI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SalI        SbfI        SexAI       
SfiI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TfiI        Tru9I       Tsp45I      
Tsp509I     Tth111I     Van91I      XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   31 enzymes) #
###################################

BamHI         893  GGATCC               G^GATCC
BbrPI         289  CACGTG               CAC^GTG
BbsI          993  GAAGAC               GAAGAC(2/6)
BlpI          306  GCTGAGC              GC^T[ACGT]AGC
BpmI          238  CTGGAG               CTGGAG(16/14)
Bpu1102I      306  GCTGAGC              GC^T[ACGT]AGC
BpuAI         993  GAAGAC               GAAGAC(2/6)
BsaBI        1575  GATCCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI         497  TCATGA               T^CATGA
BsrI          138  CCAGT                ACTGG(1/-1)
CelII         306  GCTGAGC              GC^T[ACGT]AGC
DraIII        574  CACAAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          724  GACAACGTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HincII        517  GTCAAC               GT[CT]^[GA]AC
HindII        517  GTCAAC               GT[CT]^[GA]AC
HinfI         793  GACTC                G^A[ACGT]TC
MaeIII         89  GTTAC                ^GT[ACGT]AC
MamI         1575  GATCCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI          945  CATGCGCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI          596  GCCGGC               GCC^GGC
NgoMIV        596  GCCGGC               G^CCGGC
PleI          793  GACTC                GAGTC(4/5)
PmaCI         289  CACGTG               CAC^GTG
PmlI          289  CACGTG               CAC^GTG
PpuMI        1544  GGGACCT              [GA]G^G[AT]CC[CT]
PvuII         739  CAGCTG               CAG^CTG
RcaI          497  TCATGA               T^CATGA
SacI           55  GAGCTC               GAGCT^C
SapI          204  GAAGAGC              GCTCTTC(1/4)
ScaI         1615  AGTACT               AGT^ACT
TspRI         601  CAGTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

Alw44I        333  GTGCAC               G^TGCAC
             1147  GTGCAC              
AlwNI         217  CAGAACCTG            CAG[ACGT][ACGT][ACGT]^CTG
              739  CAGCTGCTG           
ApaLI         333  GTGCAC               G^TGCAC
             1147  GTGCAC              
Asp700I       219  GAACCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              360  GAACGAGTTC          
BsaAI           4  TACGTG               [CT]AC^GT[GA]
              289  CACGTG              
BseRI        1504  GAGGAG               GAGGAG(10/8)
             1507  GAGGAG              
BspMI         370  ACCTGC               ACCTGC(4/8)
              403  GCAGGT              
BsrFI         596  GCCGGC               [GA]^CCGG[CT]
              683  ACCGGC              
Cfr10I        596  GCCGGC               [GA]^CCGG[CT]
              683  ACCGGC              
DdeI          307  CTGAG                C^T[ACGT]AG
             1447  CTGAG               
EarI          532  CTCTTC               CTCTTC(1/4)
              204  GAAGAG              
EcoT14I       143  CCAAGG               C^C[AT][AT]GG
             1178  CCAAGG              
Ksp632I       532  CTCTTC               CTCTTC(1/4)
              204  GAAGAG              
KspI          246  CCGCGG               CCGC^GG
              416  CCGCGG              
NotI          248  GCGGCCGC             GC^GGCCGC
              870  GCGGCCGC            
PstI           70  CTGCAG               CTGCA^G
              454  CTGCAG              
SacII         246  CCGCGG               CCGC^GG
              416  CCGCGG              
SfcI           70  CTGCAG               C^T[GA][CT]AG
              454  CTGCAG              
StyI          143  CCAAGG               C^C[AT][AT]GG
             1178  CCAAGG              
XcmI          269  CCAAGCTGTACCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              941  CCATCATGCGCGTGG     
XmnI          219  GAACCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              360  GAACGAGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   91 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        ApaI        AspHI       AvaI        AvaII       
BalI        BanI        BanII       BbeI        BbvI        
BcnI        BmyI        BsaHI       BsaJI       BsgI        
BsiEI       BsiHKAI     BsiYI       BslI        BsmFI       
BsoBI       Bsp1286I    BsrBI       BssHII      BssKI       
BstF5I      BstNI       BstUI       BstYI       Cac8I       
CfoI        Cfr13I      DpnII       DraII       DsaI        
EaeI        EagI        EclXI       Eco52I      EcoNI       
EcoO109I    EcoRII      FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HpaII       HphI        ItaI        
KasI        MaeII       MboI        MboII       MflI        
MluNI       MnlI        MscI        MspA1I      MspI        
MvaI        MvnI        MwoI        NarI        NciI        
NdeII       NlaIII      NlaIV       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfoI        SmaI        
TaqI        TseI        TthHB8I     XhoII       XmaCI       
XmaI        
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