Restriction map for pF1KB8709
##################################
# NO CUT ENZYMES (  130 enzymes) #
##################################

Acc65I      AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AvaI        AviII       AvrII       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaBI       BsaI        BsiEI       BsiWI       BsmBI       
BsmI        BsoBI       BspDI       BspLU11I    BsrBI       
BsrFI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstXI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      ClaI        CpoI        DraI        
DrdI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoRI       
EcoRV       EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       HhaI        HinP1I      HindIII     
HpaI        KasI        KpnI        Ksp632I     KspI        
MamI        MfeI        MluI        MunI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       PstI        
PvuI        RsrII       SacII       SapI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspI        SwaI        TfiI        
Tth111I     XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   31 enzymes) #
###################################

AatII        1110  GACGTC               GACGT^C
AccI          547  GTCGAC               GT^[AC][GT]AC
AccII         735  CGCG                 CG^CG
AccIII        801  TCCGGA               T^CCGGA
AclI          127  AACGTT               AA^CGTT
ApaI          257  GGGCCC               GGGCC^C
Asp700I       183  GAACATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI          246  TGGCCA               TGG^CCA
BamHI          69  GGATCC               G^GATCC
BfaI          160  CTAG                 C^TAG
BsaAI         769  TACGTG               [CT]AC^GT[GA]
BsaWI         801  TCCGGA               [AT]^CCGG[AT]
BseAI         801  TCCGGA               T^CCGGA
BspEI         801  TCCGGA               T^CCGGA
BspMI         332  ACCTGC               ACCTGC(4/8)
BstUI         735  CGCG                 CG^CG
DraIII        157  CACCTAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI          246  TGGCCA               [CT]^GGCC[GA]
MaeI          160  CTAG                 C^TAG
MluNI         246  TGGCCA               TGG^CCA
MroI          801  TCCGGA               T^CCGGA
MscI          246  TGGCCA               TGG^CCA
MseI          854  TTAA                 T^TAA
MvnI          735  CGCG                 CG^CG
Psp1406I      127  AACGTT               AA^CGTT
SacI          739  GAGCTC               GAGCT^C
SalI          547  GTCGAC               G^TCGAC
SmlI          168  CTTGAG               C^T[CT][GA]AG
StuI          870  AGGCCT               AGG^CCT
Tru9I         854  TTAA                 T^TAA
XmnI          183  GAACATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   39 enzymes) #
###################################

AcsI          474  GAATTT               [GA]^AATT[CT]
              987  AAATTT              
AcyI         1009  GACGCC               G[GA]^CG[CT]C
             1110  GACGTC              
AfaI           15  GTAC                 GT^AC
              897  GTAC                
AlwNI         271  CAGGGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              328  CAGCACCTG           
ApoI          474  GAATTT               [GA]^AATT[CT]
              987  AAATTT              
AspHI         711  GAGCAC               G[AT]GC[AT]^C
              739  GAGCTC              
BanI          238  GGCACC               G^G[CT][GA]CC
              690  GGTGCC              
BanII         257  GGGCCC               G[GA]GC[CT]^C
              739  GAGCTC              
BbsI           23  GTCTTC               GAAGAC(2/6)
              787  GTCTTC              
BcnI          108  CCGGG                CC^[GC]GG
              843  CCGGG               
BpuAI          23  GTCTTC               GAAGAC(2/6)
              787  GTCTTC              
BsaHI        1009  GACGCC               G[GA]^CG[CT]C
             1110  GACGTC              
BsiHKAI       711  GAGCAC               G[AT]GC[AT]^C
              739  GAGCTC              
BsmAI         304  GAGAC                GTCTC(1/5)
              673  GAGAC               
Bsp1407I       14  TGTACA               T^GTACA
              896  TGTACA              
BspHI         729  TCATGA               T^CATGA
             1154  TCATGA              
BsrGI          14  TGTACA               T^GTACA
              896  TGTACA              
BstEII        909  GGTCACC              G^GT[ACGT]ACC
             1095  GGTCACC             
BstF5I        579  CATCC                GGATG(2/0)
              795  CATCC               
BstPI         909  GGTCACC              G^GT[ACGT]ACC
             1095  GGTCACC             
BstYI          69  GGATCC               [GA]^GATC[CT]
              311  AGATCC              
DraII         110  GGGGCCT              [GA]G^G[ACGT]CC[CT]
              272  AGGGCCT             
EcoO109I      110  GGGGCCT              [GA]G^G[ACGT]CC[CT]
              272  AGGGCCT             
EcoO65I       909  GGTCACC              G^GT[ACGT]ACC
             1095  GGTCACC             
FokI          579  CATCC                GGATG(9/13)
              795  CATCC               
HgaI          733  GACGC                GACGC(5/10)
             1009  GACGC               
Hin1I        1009  GACGCC               G[GA]^CG[CT]C
             1110  GACGTC              
MflI           69  GGATCC               [GA]^GATC[CT]
              311  AGATCC              
MwoI          239  GCACCTGTGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              872  GCCTGACCAGC         
NciI          108  CCGGG                CC^[GC]GG
              843  CCGGG               
PflMI          60  CCAGGCCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              270  CCAGGGCCTGG         
RcaI          729  TCATGA               T^CATGA
             1154  TCATGA              
RsaI           15  GTAC                 GT^AC
              897  GTAC                
SfaNI         206  GCATC                GCATC(5/9)
              998  GCATC               
SspBI          14  TGTACA               T^GTACA
              896  TGTACA              
Tsp45I        910  GTCAC                ^GT[GC]AC
             1096  GTCAC               
Van91I         60  CCAGGCCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              270  CCAGGGCCTGG         
XcmI          578  CCATCCTCGTCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              878  CCAGCAGCCAGGTGG     
XhoII          69  GGATCC               [GA]^GATC[CT]
              311  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   58 enzymes) #
#########################################

AciI        AluI        AlwI        AvaII       BbvI        
BmyI        BpmI        BsaJI       BseRI       BsgI        
BsiYI       BslI        BsmFI       Bsp1286I    BsrDI       
BsrI        BssKI       BstNI       Cac8I       Cfr13I      
DdeI        DpnII       DsaI        EcoRII      EcoT14I     
Fnu4HI      HaeIII      HapII       HincII      HindII      
HinfI       HpaII       HphI        ItaI        MaeII       
MaeIII      MboI        MboII       MnlI        MslI        
MspA1I      MspI        MvaI        NcoI        NdeII       
NlaIII      NlaIV       PleI        PvuII       Sau3AI      
Sau96I      ScrFI       StyI        TaqI        TseI        
Tsp509I     TspRI       TthHB8I     
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