Restriction map for pF1KB8709
 (Linear) (Six-base) MAPSORT of: KIBB8709.seq  Check: 2115  from: 1  to: 1215



KIBB8709.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     341    1215 
   Size:        341     874
 
AatII G_ACGT'C

Cuts at:      0    1114    1215 
   Size:       1114     101
 
AccI GT'mk_AC

Cuts at:      0     548    1215 
   Size:        548     667
 
AclI AA'CG_TT

Cuts at:      0     128    1215 
   Size:        128    1087
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     104     126    1039    1215 
   Size:        104      22     913     176

  Fragments arranged by size: 

                 913     176     104      22
 
AlwNI CAG_nnn'CTG

Cuts at:      0     276     333    1215 
   Size:        276      57     882

  Fragments arranged by size: 

                 882     276      57
 
ApaI G_GGCC'C

Cuts at:      0     261    1215 
   Size:        261     954
 
ApoI r'AATT_y

Cuts at:      0     474     987    1215 
   Size:        474     513     228

  Fragments arranged by size: 

                 513     474     228
 
BamHI G'GATC_C

Cuts at:      0      69    1215 
   Size:         69    1146
 
BanI G'GyrC_C

Cuts at:      0     238     690    1215 
   Size:        238     452     525

  Fragments arranged by size: 

                 525     452     238
 
BanII G_rGCy'C

Cuts at:      0     261     743    1215 
   Size:        261     482     472

  Fragments arranged by size: 

                 482     472     261
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      16     780    1215 
   Size:         16     764     435

  Fragments arranged by size: 

                 764     435      16
 
Bme1580I G_kGCm'C

Cuts at:      0     241     261     695    1215 
   Size:        241      20     434     520

  Fragments arranged by size: 

                 520     434     241      20
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     495    1100    1204    1215 
   Size:        495     605     104      11

  Fragments arranged by size: 

                 605     495     104      11
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     444    1215 
   Size:        444     771
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     253     430     439     941     967    1197    1215 
   Size:        253     177       9     502      26     230      18

  Fragments arranged by size: 

                 502     253     230     177      26      18       9
 
Bpu10I CC'TnA_GC

Cuts at:      0     219    1215 
   Size:        219     996
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     189    1215 
   Size:        189    1026
 
BsaAI yAC'GTr

Cuts at:      0     771    1215 
   Size:        771     444
 
BsaHI Gr'CG_yC

Cuts at:      0    1010    1111    1215 
   Size:       1010     101     104

  Fragments arranged by size: 

                1010     104     101
 
BsaWI w'CCGG_w

Cuts at:      0     801    1215 
   Size:        801     414
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     667    1078    1215 
   Size:        667     411     137

  Fragments arranged by size: 

                 667     411     137
 
BseYI C'CCAG_C

Cuts at:      0     260     573    1013    1215 
   Size:        260     313     440     202

  Fragments arranged by size: 

                 440     313     260     202
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     319     778     818    1215 
   Size:        319     459      40     397

  Fragments arranged by size: 

                 459     397     319      40
 
BsiHKAI G_wGCw'C

Cuts at:      0     715     743    1215 
   Size:        715      28     472

  Fragments arranged by size: 

                 715     472      28
 
Bsp1286I G_dGCh'C

Cuts at:      0     241     261     695     715     743    1215 
   Size:        241      20     434      20      28     472

  Fragments arranged by size: 

                 472     434     241      28      20      20
 
BspEI T'CCGG_A

Cuts at:      0     801    1215 
   Size:        801     414
 
BspHI T'CATG_A

Cuts at:      0     729    1154    1215 
   Size:        729     425      61

  Fragments arranged by size: 

                 729     425      61
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     341    1215 
   Size:        341     874
 
BsrDI GCAATG_nn'

Cuts at:      0     390     783     959    1215 
   Size:        390     393     176     256

  Fragments arranged by size: 

                 393     390     256     176
 
BsrGI T'GTAC_A

Cuts at:      0      14     896    1215 
   Size:         14     882     319

  Fragments arranged by size: 

                 882     319      14
 
BstEII G'GTnAC_C

Cuts at:      0     909    1095    1215 
   Size:        909     186     120

  Fragments arranged by size: 

                 909     186     120
 
BstYI r'GATC_y

Cuts at:      0      69     311    1215 
   Size:         69     242     904

  Fragments arranged by size: 

                 904     242      69
 
BtgI C'CryG_G

Cuts at:      0      65     212     377    1215 
   Size:         65     147     165     838

  Fragments arranged by size: 

                 838     165     147      65
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     813    1215 
   Size:        813     402
 
DraIII CAC_nnn'GTG

Cuts at:      0     162    1215 
   Size:        162    1053
 
EaeI y'GGCC_r

Cuts at:      0     246    1215 
   Size:        246     969
 
EcoICRI GAG'CTC

Cuts at:      0     741    1215 
   Size:        741     474
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     104     126     253     430     439     941     967    1039 
   Size:        104      22     127     177       9     502      26      72

Cuts at:   1039    1197    1215 
   Size:        158      18

  Fragments arranged by size: 

                 502     177     158     127     104      72      26      22
                  18       9
 
EcoO109I rG'GnC_Cy

Cuts at:      0     111     273    1215 
   Size:        111     162     942

  Fragments arranged by size: 

                 942     162     111
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      87     119    1215 
   Size:         87      32    1096

  Fragments arranged by size: 

                1096      87      32
 
HincII GTy'rAC

Cuts at:      0     132     549     594    1215 
   Size:        132     417      45     621

  Fragments arranged by size: 

                 621     417     132      45
 
MscI TGG'CCA

Cuts at:      0     248    1215 
   Size:        248     967
 
MslI CAynn'nnrTG

Cuts at:      0     211     449    1153    1215 
   Size:        211     238     704      62

  Fragments arranged by size: 

                 704     238     211      62
 
MspA1I CmG'CkG

Cuts at:      0      44     753    1017    1081    1215 
   Size:         44     709     264      64     134

  Fragments arranged by size: 

                 709     264     134      64      44
 
NcoI C'CATG_G

Cuts at:      0      65     212     377    1215 
   Size:         65     147     165     838

  Fragments arranged by size: 

                 838     165     147      65
 
PasI CC'CwG_GG

Cuts at:      0     234    1215 
   Size:        234     981
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      66     276    1215 
   Size:         66     210     939

  Fragments arranged by size: 

                 939     210      66
 
PfoI T'CCnGG_A

Cuts at:      0     479    1215 
   Size:        479     736
 
PspOMI G'GGCC_C

Cuts at:      0     257    1215 
   Size:        257     958
 
PvuII CAG'CTG

Cuts at:      0     753    1017    1081    1215 
   Size:        753     264      64     134

  Fragments arranged by size: 

                 753     264     134      64
 
SacI G_AGCT'C

Cuts at:      0     743    1215 
   Size:        743     472
 
SalI G'TCGA_C

Cuts at:      0     547    1215 
   Size:        547     668
 
SmlI C'TyrA_G

Cuts at:      0     168    1215 
   Size:        168    1047
 
StuI AGG'CCT

Cuts at:      0     872    1215 
   Size:        872     343
 
StyI C'CwwG_G

Cuts at:      0      65     212     377    1215 
   Size:         65     147     165     838

  Fragments arranged by size: 

                 838     165     147      65
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1018    1215 
   Size:       1018     197
 
TatI w'GTAC_w

Cuts at:      0      14     896    1215 
   Size:         14     882     319

  Fragments arranged by size: 

                 882     319      14
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     585     885    1215 
   Size:        585     300     330

  Fragments arranged by size: 

                 585     330     300
 
XmnI GAAnn'nnTTC

Cuts at:      0     187    1215 
   Size:        187    1028
 
ZraI GAC'GTC

Cuts at:      0    1112    1215 
   Size:       1112     103
 

 Enzymes that do cut:

     AarI    AatII     AccI     AclI     AcuI    AlwNI     ApaI     ApoI
    BamHI     BanI    BanII     BbsI Bme1580I     BmrI     BplI     BpmI
   Bpu10I    BpuEI    BsaAI    BsaHI    BsaWI    BseRI    BseYI     BsgI
  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrDI    BsrGI   BstEII
    BstYI     BtgI    BtgZI   DraIII     EaeI  EcoICRI  Eco57MI EcoO109I
    Hin4I   HincII     MscI     MslI   MspA1I     NcoI     PasI    PflMI
     PfoI   PspOMI    PvuII     SacI     SalI     SmlI     StuI     StyI
    TaqII     TatI     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AfeI    AflII   AflIII     AgeI     AhdI     AleI     AlfI
     AloI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmtI     BsaI    BsaBI    BsaXI    BsiEI    BsiWI
     BsmI    BsmBI    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstBI
    BstXI  BstZ17I   Bsu36I     BtsI     ClaI    CspCI     DraI     DrdI
     EagI     EarI     EciI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PciI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    RsrII
    SacII    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI     TstI  Tth111I     XbaI     XhoI     XmaI
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