Restriction map for pF1KB8689
##################################
# NO CUT ENZYMES (  104 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AhdI        AlwNI       ApaI        
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvrII       BamHI       
BciVI       BclI        BfrI        BlnI        BsaBI       
BseAI       BsiWI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspLU11I    BsrDI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstZ17I     ClaI        CpoI        DraI        DraIII      
DrdI        Eam1105I    EcoNI       EcoRI       EcoRV       
EcoT22I     FbaI        FseI        HincII      HindII      
HindIII     HpaI        KpnI        KspI        MamI        
MfeI        MroI        MseI        MunI        NaeI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
PvuII       RsrII       SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfuI        SmaI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
SwaI        Tru9I       Tth111I     XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AflIII       1187  ACGCGT               A^C[GA][CT]GT
AgeI         1009  ACCGGT               A^CCGGT
Alw44I        252  GTGCAC               G^TGCAC
AocI          510  CCTCAGG              CC^T[ACGT]AGG
Aor51HI       410  AGCGCT               AGC^GCT
ApaLI         252  GTGCAC               G^TGCAC
BalI         1082  TGGCCA               TGG^CCA
BanII         712  GAGCTC               G[GA]GC[CT]^C
BbrPI         805  CACGTG               CAC^GTG
BbsI         1135  GTCTTC               GAAGAC(2/6)
BbvCI        1000  GCTGAGG              CCTCAGC(-5/-2)
BfaI           22  CTAG                 C^TAG
BglII         428  AGATCT               A^GATCT
BlpI          620  GCTCAGC              GC^T[ACGT]AGC
BpmI          171  CTGGAG               CTGGAG(16/14)
Bpu1102I      620  GCTCAGC              GC^T[ACGT]AGC
BpuAI        1135  GTCTTC               GAAGAC(2/6)
BsaAI         805  CACGTG               [CT]AC^GT[GA]
BsaWI        1009  ACCGGT               [AT]^CCGG[AT]
BseRI        1144  CTCCTC               GAGGAG(10/8)
BspHI         326  TCATGA               T^CATGA
BstXI         836  CCACCATCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        510  CCTCAGG              CC^T[ACGT]AGG
CelII         620  GCTCAGC              GC^T[ACGT]AGC
Eco47III      410  AGCGCT               AGC^GCT
Eco81I        510  CCTCAGG              CC^T[ACGT]AGG
HgaI         1133  GCGTC                GACGC(5/10)
MaeI           22  CTAG                 C^TAG
MluI         1187  ACGCGT               A^CGCGT
MluNI        1082  TGGCCA               TGG^CCA
MscI         1082  TGGCCA               TGG^CCA
MspA1I        623  CAGCGG               C[AC]G^C[GT]G
NcoI          309  CCATGG               C^CATGG
NspI          884  GCATGC               [GA]CATG^[CT]
PflMI         801  CCAGCACGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        1009  ACCGGT               A^CCGGT
PleI         1248  GACTC                GAGTC(4/5)
PmaCI         805  CACGTG               CAC^GTG
PmlI          805  CACGTG               CAC^GTG
PstI          114  CTGCAG               CTGCA^G
RcaI          326  TCATGA               T^CATGA
SacI          712  GAGCTC               GAGCT^C
SapI          444  GAAGAGC              GCTCTTC(1/4)
SfaNI        1206  GATGC                GCATC(5/9)
SfcI          114  CTGCAG               C^T[GA][CT]AG
SgrAI        1008  CACCGGTG             C[GA]^CCGG[CT]G
SmlI          151  CTCAAG               C^T[CT][GA]AG
SphI          884  GCATGC               GCATG^C
TfiI           86  GATTC                G^A[AT]TC
Tsp509I       577  AATT                 ^AATT
Van91I        801  CCAGCACGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AccII         257  CGCG                 CG^CG
             1188  CGCG                
AcyI          553  GGCGCC               G[GA]^CG[CT]C
             1309  GGCGCC              
AlwI          147  GATCC                GGATC(4/5)
              813  GATCC               
AspHI         252  GTGCAC               G[AT]GC[AT]^C
              712  GAGCTC              
AviII         236  TGCGCA               TGC^GCA
              377  TGCGCA              
BbeI          553  GGCGCC               GGCGC^C
             1309  GGCGCC              
BcgI          219  CGAGTTCTGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              833  GCACCACCATCG        
BglI          851  GCCAGGTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              944  GCCTGCGGGGC         
BsaHI         553  GGCGCC               G[GA]^CG[CT]C
             1309  GGCGCC              
BsaI         1057  GAGACC               GGTCTC(1/5)
              931  GGTCTC              
BsiHKAI       252  GTGCAC               G[AT]GC[AT]^C
              712  GAGCTC              
BsrBI         562  CCGCTC               CCGCTC(-3/-3)
             1321  CCGCTC              
BsrI          917  ACTGG                ACTGG(1/-1)
             1173  ACTGG               
BstEII        283  GGTGACC              G^GT[ACGT]ACC
              594  GGTCACC             
BstPI         283  GGTGACC              G^GT[ACGT]ACC
              594  GGTCACC             
BstUI         257  CGCG                 CG^CG
             1188  CGCG                
EarI          493  CTCTTC               CTCTTC(1/4)
              444  GAAGAG              
EcoO65I       283  GGTGACC              G^GT[ACGT]ACC
              594  GGTCACC             
FspI          236  TGCGCA               TGC^GCA
              377  TGCGCA              
Hin1I         553  GGCGCC               G[GA]^CG[CT]C
             1309  GGCGCC              
HinfI          86  GATTC                G^A[ACGT]TC
             1248  GACTC               
KasI          553  GGCGCC               G^GCGCC
             1309  GGCGCC              
Ksp632I       493  CTCTTC               CTCTTC(1/4)
              444  GAAGAG              
MvnI          257  CGCG                 CG^CG
             1188  CGCG                
NarI          553  GGCGCC               GG^CGCC
             1309  GGCGCC              
SfoI          553  GGCGCC               GGC^GCC
             1309  GGCGCC              
StuI          983  AGGCCT               AGG^CCT
             1127  AGGCCT              
Tsp45I        284  GTGAC                ^GT[GC]AC
              595  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   75 enzymes) #
#########################################

AciI        AfaI        AluI        AvaI        AvaII       
BanI        BbvI        BcnI        BmyI        BsaJI       
BsgI        BsiEI       BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrFI       
BssKI       BstF5I      BstNI       BstYI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EagI        EclXI       
Eco52I      EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        HinP1I      HpaII       HphI        ItaI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MslI        MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PpuMI       
RsaI        Sau3AI      Sau96I      ScrFI       StyI        
TaqI        TseI        TspRI       TthHB8I     XhoII       
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