Restriction map for pF1KB8678
##################################
# NO CUT ENZYMES (  131 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwNI       Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvaI        AvrII       BalI        BciVI       
BfrI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BsaBI       BseAI       BsiEI       
BsiWI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrDI       BsrFI       
BsrGI       BsrI        BssHII      Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     CelII       Cfr10I      
ClaI        CpoI        DraIII      DrdI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoO65I     EcoRI       EcoT22I     FseI        HincII      
HindII      HindIII     HpaI        KpnI        KspI        
MamI        MfeI        MluI        MluNI       MroI        
MscI        MunI        NaeI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SbfI        SexAI       SfiI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tsp509I     TspRI       Tth111I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AatII         565  GACGTC               GACGT^C
AccII         555  CGCG                 CG^CG
AocI          997  CCTCAGG              CC^T[ACGT]AGG
ApaI          770  GGGCCC               GGGCC^C
AviII         542  TGCGCA               TGC^GCA
BamHI          49  GGATCC               G^GATCC
BbeI          336  GGCGCC               GGCGC^C
BbrPI        1017  CACGTG               CAC^GTG
BbsI         1093  GAAGAC               GAAGAC(2/6)
BbvCI         184  GCTGAGG              CCTCAGC(-5/-2)
BcgI          545  GCATCGACATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI           81  TGATCA               T^GATCA
BpuAI        1093  GAAGAC               GAAGAC(2/6)
BsaAI        1017  CACGTG               [CT]AC^GT[GA]
BsaWI          52  TCCGGT               [AT]^CCGG[AT]
BsgI          992  CTGCAC               GTGCAG(16/14)
BsmFI         563  GGGAC                GGGAC(10/14)
BsrBI         404  CCGCTC               CCGCTC(-3/-3)
BssSI         826  CACGAG               CACGAG(-5/-1)
BstAPI        315  GCATCCTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         555  CGCG                 CG^CG
BstXI         458  CCACAGGGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        997  CCTCAGG              CC^T[ACGT]AGG
DraI          342  TTTAAA               TTT^AAA
DsaI          221  CCATGG               C^C[GA][CT]GG
EarI          784  CTCTTC               CTCTTC(1/4)
Eco81I        997  CCTCAGG              CC^T[ACGT]AGG
EcoRV         987  GATATC               GAT^ATC
EcoT14I       221  CCATGG               C^C[AT][AT]GG
FbaI           81  TGATCA               T^GATCA
FspI          542  TGCGCA               TGC^GCA
HaeII         336  GGCGCC               [GA]GCGC^[CT]
HgaI          696  GACGC                GACGC(5/10)
KasI          336  GGCGCC               G^GCGCC
Ksp632I       784  CTCTTC               CTCTTC(1/4)
MspA1I        292  CAGCTG               C[AC]G^C[GT]G
MvnI          555  CGCG                 CG^CG
NarI          336  GGCGCC               GG^CGCC
NcoI          221  CCATGG               C^CATGG
PmaCI        1017  CACGTG               CAC^GTG
PmlI         1017  CACGTG               CAC^GTG
PpuMI         830  AGGTCCT              [GA]G^G[AT]CC[CT]
PstI          295  CTGCAG               CTGCA^G
PvuII         292  CAGCTG               CAG^CTG
SapI          783  GCTCTTC              GCTCTTC(1/4)
ScaI          719  AGTACT               AGT^ACT
SfoI          336  GGCGCC               GGC^GCC
StyI          221  CCATGG               C^C[AT][AT]GG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AcyI          336  GGCGCC               G[GA]^CG[CT]C
              565  GACGTC              
AfaI          430  GTAC                 GT^AC
              720  GTAC                
AspHI         190  GAGCAC               G[AT]GC[AT]^C
              322  GTGCTC              
BanI          336  GGCGCC               G^G[CT][GA]CC
             1099  GGTGCC              
BfaI          700  CTAG                 C^TAG
              961  CTAG                
BsaHI         336  GGCGCC               G[GA]^CG[CT]C
              565  GACGTC              
BsaI          388  GAGACC               GGTCTC(1/5)
             1071  GGTCTC              
BsiHKAI       190  GAGCAC               G[AT]GC[AT]^C
              322  GTGCTC              
BsmBI         567  CGTCTC               CGTCTC(1/5)
              694  GAGACG              
BstNI         834  CCTGG                CC^[AT]GG
             1047  CCTGG               
BstYI          49  GGATCC               [GA]^GATC[CT]
               86  AGATCC              
DraII         770  GGGCCCC              [GA]G^G[ACGT]CC[CT]
              830  AGGTCCT             
EcoNI         327  CCTGACTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              591  CCTGCGTAAGG         
EcoO109I      770  GGGCCCC              [GA]G^G[ACGT]CC[CT]
              830  AGGTCCT             
EcoRII        834  CCTGG                ^CC[AT]GG
             1047  CCTGG               
FauI          403  CCCGC                CCCGC(4/6)
              556  GCGGG               
Hin1I         336  GGCGCC               G[GA]^CG[CT]C
              565  GACGTC              
MaeI          700  CTAG                 C^TAG
              961  CTAG                
MflI           49  GGATCC               [GA]^GATC[CT]
               86  AGATCC              
MvaI          834  CCTGG                CC^[AT]GG
             1047  CCTGG               
PleI            4  GAGTC                GAGTC(4/5)
              488  GAGTC               
RsaI          430  GTAC                 GT^AC
              720  GTAC                
SfcI          295  CTGCAG               C^T[GA][CT]AG
              446  CTACAG              
TfiI          475  GATTC                G^A[AT]TC
              764  GATTC               
Tsp45I        490  GTCAC                ^GT[GC]AC
              932  GTGAC               
XhoII          49  GGATCC               [GA]^GATC[CT]
               86  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   53 enzymes) #
#########################################

AciI        AluI        AlwI        AvaII       BanII       
BbvI        BcnI        BmyI        BsaJI       BseRI       
BsiYI       BslI        BsmAI       Bsp1286I    BspMI       
BssKI       BstF5I      Cac8I       CfoI        Cfr13I      
DdeI        DpnII       Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        MaeII       MaeIII      MboI        
MboII       MnlI        MseI        MslI        MspI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
Sau3AI      Sau96I      ScrFI       SfaNI       TaqI        
Tru9I       TseI        TthHB8I     
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com