Restriction map for pF1KB8678
 (Linear) (Six-base) MAPSORT of: KIBB8678.seq  Check: 2289  from: 1  to: 1128



KIBB8678.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:07  ..

AatII G_ACGT'C

Cuts at:      0     569    1128 
   Size:        569     559
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     771    1128 
   Size:        771     357
 
AleI CACnn'nnGTG

Cuts at:      0     134     463    1128 
   Size:        134     329     665

  Fragments arranged by size: 

                 665     329     134
 
ApaI G_GGCC'C

Cuts at:      0     774    1128 
   Size:        774     354
 
BamHI G'GATC_C

Cuts at:      0      49    1128 
   Size:         49    1079
 
BanI G'GyrC_C

Cuts at:      0     336    1099    1128 
   Size:        336     763      29

  Fragments arranged by size: 

                 763     336      29
 
BanII G_rGCy'C

Cuts at:      0     153     774    1033    1128 
   Size:        153     621     259      95

  Fragments arranged by size: 

                 621     259     153      95
 
BbeI G_GCGC'C

Cuts at:      0     340    1128 
   Size:        340     788
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1100    1128 
   Size:       1100      28
 
BbvCI CC'TCA_GC

Cuts at:      0     185    1128 
   Size:        185     943
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     534     568    1128 
   Size:        534      34     560

  Fragments arranged by size: 

                 560     534      34
 
BclI T'GATC_A

Cuts at:      0      81    1128 
   Size:         81    1047
 
Bme1580I G_kGCm'C

Cuts at:      0     404     506     774    1104    1128 
   Size:        404     102     268     330      24

  Fragments arranged by size: 

                 404     330     268     102      24
 
Bpu10I CC'TnA_GC

Cuts at:      0     185    1128 
   Size:        185     943
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     382    1077    1128 
   Size:        382     695      51

  Fragments arranged by size: 

                 695     382      51
 
BsaAI yAC'GTr

Cuts at:      0    1019    1128 
   Size:       1019     109
 
BsaHI Gr'CG_yC

Cuts at:      0     337     566    1128 
   Size:        337     229     562

  Fragments arranged by size: 

                 562     337     229
 
BsaWI w'CCGG_w

Cuts at:      0      52    1128 
   Size:         52    1076
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     994    1024    1128 
   Size:        994      30     104

  Fragments arranged by size: 

                 994     104      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     202     325     823    1128 
   Size:        202     123     498     305

  Fragments arranged by size: 

                 498     305     202     123
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     977    1128 
   Size:        977     151
 
BsiHKAI G_wGCw'C

Cuts at:      0     194     326    1128 
   Size:        194     132     802

  Fragments arranged by size: 

                 802     194     132
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     573     688    1128 
   Size:        573     115     440

  Fragments arranged by size: 

                 573     440     115
 
Bsp1286I G_dGCh'C

Cuts at:      0     153     194     326     404     506     774    1033    1104 
   Size:        153      41     132      78     102     268     259      71

Cuts at:   1104    1128 
   Size:         24

  Fragments arranged by size: 

                 268     259     153     132     102      78      71      41
                  24
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     599     734     875    1128 
   Size:        599     135     141     253

  Fragments arranged by size: 

                 599     253     141     135
 
BsrBI CCG'CTC

Cuts at:      0     406    1128 
   Size:        406     722
 
BssSI C'ACGA_G

Cuts at:      0     826    1128 
   Size:        826     302
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     321    1128 
   Size:        321     807
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     465    1128 
   Size:        465     663
 
BstYI r'GATC_y

Cuts at:      0      49      86    1128 
   Size:         49      37    1042

  Fragments arranged by size: 

                1042      49      37
 
Bsu36I CC'TnA_GG

Cuts at:      0     998    1128 
   Size:        998     130
 
BtgI C'CryG_G

Cuts at:      0     221    1128 
   Size:        221     907
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     537    1128 
   Size:        537     591
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      95     130    1005    1040    1128 
   Size:         95      35     875      35      88

  Fragments arranged by size: 

                 875      95      88      35      35
 
DraI TTT'AAA

Cuts at:      0     344    1128 
   Size:        344     784
 
EarI CTCTTCn'nnn_

Cuts at:      0     790    1128 
   Size:        790     338
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     771    1128 
   Size:        771     357
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     331     595    1128 
   Size:        331     264     533

  Fragments arranged by size: 

                 533     331     264
 
EcoO109I rG'GnC_Cy

Cuts at:      0     771     831    1128 
   Size:        771      60     297

  Fragments arranged by size: 

                 771     297      60
 
EcoRV GAT'ATC

Cuts at:      0     989    1128 
   Size:        989     139
 
FspI TGC'GCA

Cuts at:      0     544    1128 
   Size:        544     584
 
FspAI rTGC'GCAy

Cuts at:      0     544    1128 
   Size:        544     584
 
HaeII r_GCGC'y

Cuts at:      0     340    1128 
   Size:        340     788
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      34      66     601     633    1128 
   Size:         34      32     535      32     495

  Fragments arranged by size: 

                 535     495      34      32      32
 
KasI G'GCGC_C

Cuts at:      0     336    1128 
   Size:        336     792
 
MslI CAynn'nnrTG

Cuts at:      0     134     418     463    1128 
   Size:        134     284      45     665

  Fragments arranged by size: 

                 665     284     134      45
 
MspA1I CmG'CkG

Cuts at:      0     294    1128 
   Size:        294     834
 
NarI GG'CG_CC

Cuts at:      0     337    1128 
   Size:        337     791
 
NcoI C'CATG_G

Cuts at:      0     221    1128 
   Size:        221     907
 
PmlI CAC'GTG

Cuts at:      0    1019    1128 
   Size:       1019     109
 
PpuMI rG'GwC_Cy

Cuts at:      0     831    1128 
   Size:        831     297
 
PspOMI G'GGCC_C

Cuts at:      0     770    1128 
   Size:        770     358
 
PstI C_TGCA'G

Cuts at:      0     299    1128 
   Size:        299     829
 
PvuII CAG'CTG

Cuts at:      0     294    1128 
   Size:        294     834
 
SapI GCTCTTCn'nnn_

Cuts at:      0     790    1128 
   Size:        790     338
 
ScaI AGT'ACT

Cuts at:      0     721    1128 
   Size:        721     407
 
SfcI C'TryA_G

Cuts at:      0     295     446    1128 
   Size:        295     151     682

  Fragments arranged by size: 

                 682     295     151
 
SfoI GGC'GCC

Cuts at:      0     338    1128 
   Size:        338     790
 
StyI C'CwwG_G

Cuts at:      0     221    1128 
   Size:        221     907
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     508    1128 
   Size:        508     620
 
TatI w'GTAC_w

Cuts at:      0     429     719    1128 
   Size:        429     290     409

  Fragments arranged by size: 

                 429     409     290
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      41      73    1128 
   Size:         41      32    1055

  Fragments arranged by size: 

                1055      41      32
 
ZraI GAC'GTC

Cuts at:      0     567    1128 
   Size:        567     561
 

 Enzymes that do cut:

    AatII     AcuI     AleI     ApaI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI     BclI Bme1580I   Bpu10I     BsaI    BsaAI
    BsaHI    BsaWI    BsaXI    BseRI     BsgI  BsiHKAI    BsmBI Bsp1286I
    BspMI    BsrBI    BssSI   BstAPI    BstXI    BstYI   Bsu36I     BtgI
    BtgZI    CspCI     DraI     EarI  Eco57MI    EcoNI EcoO109I    EcoRV
     FspI    FspAI    HaeII    Hin4I     KasI     MslI   MspA1I     NarI
     NcoI     PmlI    PpuMI   PspOMI     PstI    PvuII     SapI     ScaI
     SfcI     SfoI     StyI    TaqII     TatI     TstI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    AlwNI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BciVI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI    BpuEI    BsaBI
    BseYI    BsiEI    BsiWI     BsmI    BspEI    BspHI    BsrDI    BsrFI
    BsrGI   BssHII    BstBI   BstEII  BstZ17I     BtsI     ClaI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI    EcoRI     FalI     FseI
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
     NaeI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SbfI    SexAI     SfiI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI
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