Restriction map for pF1KB8677
##################################
# NO CUT ENZYMES (  124 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AgeI        AhdI        Alw44I      AlwI        
Aor51HI     ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AviII       AvrII       BamHI       
BbeI        BbvCI       BcgI        BfaI        BfrI        
BlnI        BlpI        Bpu1102I    BsaHI       BsaWI       
BseAI       BseRI       BsgI        BsiWI       BsmI        
BspDI       BspEI       BspHI       BspMI       BsrDI       
BsrFI       BssHII      Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstXI       BstZ17I     CelII       
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        Eam1105I    EarI        Eco47III    EcoNI       
EcoO65I     EcoRV       EcoT22I     FseI        FspI        
Hin1I       HindIII     HpaI        KasI        KpnI        
Ksp632I     KspI        MaeI        MfeI        MluI        
MroI        MseI        MunI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflMI       
PinAI       PleI        PmeI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        PvuII       RcaI        
RsrII       SacII       SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspI        SwaI        Tru9I       Van91I      
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   60 enzymes) #
###################################

AccI          126  GTCGAC               GT^[AC][GT]AC
AccII         140  CGCG                 CG^CG
AcsI          768  GAATTC               [GA]^AATT[CT]
AocI         1088  CCTCAGG              CC^T[ACGT]AGG
ApoI          768  GAATTC               [GA]^AATT[CT]
Asp700I       592  GAAAGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         685  GAGCTC               G[AT]GC[AT]^C
AspI          520  GACTATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          638  CTCGGG               C^[CT]CG[GA]G
AvaII          50  GGTCC                G^G[AT]CC
BbrPI         775  CACGTG               CAC^GTG
BciVI         797  GTATCC               GTATCC(6/5)
BclI          561  TGATCA               T^GATCA
BglI           16  GCCCGGCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         445  AGATCT               A^GATCT
BsaBI         694  GATGTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          232  GAGACC               GGTCTC(1/5)
BsiHKAI       685  GAGCTC               G[AT]GC[AT]^C
BsoBI         638  CTCGGG               C^[CT]CG[GA]G
Bsp1407I       10  TGTACA               T^GTACA
BspLU11I      986  ACATGT               A^CATGT
BsrGI          10  TGTACA               T^GTACA
BssSI         419  CTCGTG               CACGAG(-5/-1)
BstUI         140  CGCG                 CG^CG
BstYI         445  AGATCT               [GA]^GATC[CT]
Bsu36I       1088  CCTCAGG              CC^T[ACGT]AGG
CfoI          789  GCGC                 GCG^C
EagI           98  CGGCCG               C^GGCCG
EclXI          98  CGGCCG               C^GGCCG
Eco52I         98  CGGCCG               C^GGCCG
Eco81I       1088  CCTCAGG              CC^T[ACGT]AGG
EcoRI         768  GAATTC               G^AATTC
FauI           21  GCGGG                CCCGC(4/6)
FbaI          561  TGATCA               T^GATCA
HaeII         788  GGCGCT               [GA]GCGC^[CT]
HhaI          789  GCGC                 GCG^C
HinP1I        789  GCGC                 G^CGC
HincII        126  GTCGAC               GT[CT]^[GA]AC
HindII        126  GTCGAC               GT[CT]^[GA]AC
MamI          694  GATGTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          445  AGATCT               [GA]^GATC[CT]
MspA1I         42  CCGCTG               C[AC]G^C[GT]G
MvnI          140  CGCG                 CG^CG
NcoI          360  CCATGG               C^CATGG
NspI          986  ACATGT               [GA]CATG^[CT]
PflFI         520  GACTATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI         775  CACGTG               CAC^GTG
PmlI          775  CACGTG               CAC^GTG
SacI          685  GAGCTC               GAGCT^C
SalI          126  GTCGAC               G^TCGAC
SfaNI         415  GATGC                GCATC(5/9)
SspBI          10  TGTACA               T^GTACA
StuI         1092  AGGCCT               AGG^CCT
TaqI          127  TCGA                 T^CGA
Tsp509I       769  AATT                 ^AATT
Tth111I       520  GACTATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       127  TCGA                 T^CGA
XcmI          506  CCATCCCAGGCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         445  AGATCT               [GA]^GATC[CT]
XmnI          592  GAAAGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

BalI           76  TGGCCA               TGG^CCA
              357  TGGCCA              
BbsI          535  GAAGAC               GAAGAC(2/6)
             1077  GAAGAC              
BpuAI         535  GAAGAC               GAAGAC(2/6)
             1077  GAAGAC              
BsaAI         722  TACGTG               [CT]AC^GT[GA]
              775  CACGTG              
BsiEI          55  CGGTCG               CG[GA][CT]^CG
               98  CGGCCG              
BsmBI         960  CGTCTC               CGTCTC(1/5)
              643  GAGACG              
BsmFI          51  GTCCC                GGGAC(10/14)
              156  GTCCC               
BsrBI          85  CCGCTC               CCGCTC(-3/-3)
              841  GAGCGG              
DpnII         446  GATC                 ^GATC
              562  GATC                
DsaI           32  CCGTGG               C^C[GA][CT]GG
              360  CCATGG              
HinfI         669  GAATC                G^A[ACGT]TC
             1041  GATTC               
HphI          375  GGTGA                GGTGA(8/7)
              672  TCACC               
MboI          446  GATC                 ^GATC
              562  GATC                
MluNI          76  TGGCCA               TGG^CCA
              357  TGGCCA              
MscI           76  TGGCCA               TGG^CCA
              357  TGGCCA              
MslI          747  CATGGGCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1027  CACTTTGATG          
NdeII         446  GATC                 ^GATC
              562  GATC                
PstI          326  CTGCAG               CTGCA^G
              967  CTGCAG              
Sau3AI        446  GATC                 ^GATC
              562  GATC                
SfcI          326  CTGCAG               C^T[GA][CT]AG
              967  CTGCAG              
TfiI          669  GAATC                G^A[AT]TC
             1041  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (   53 enzymes) #
#########################################

AciI        AfaI        AflIII      AluI        AlwNI       
ApaI        BanI        BanII       BbvI        BcnI        
BmyI        BpmI        BsaJI       BsiYI       BslI        
BsmAI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       Cac8I       Cfr13I      DdeI        DraII       
EaeI        EcoO109I    EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HgaI        HpaII       
ItaI        MaeII       MaeIII      MboII       MnlI        
MspI        MvaI        MwoI        NciI        NlaIII      
NlaIV       RsaI        Sau96I      ScrFI       StyI        
TseI        Tsp45I      TspRI       
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