Restriction map for pF1KB8670
##################################
# NO CUT ENZYMES (  103 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AflII       
AgeI        AhdI        Alw44I      AlwNI       Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AviII       AvrII       BbrPI       
BbvCI       BciVI       BclI        BfrI        BglI        
BglII       BlnI        BsaBI       BsaWI       BseAI       
BseRI       BsiWI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BsrGI       BssSI       Bst1107I    
BstAPI      BstBI       BstZ17I     ClaI        CpoI        
DraI        DrdI        Eam1105I    EarI        Eco47III    
EcoNI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HindIII     HpaI        KpnI        Ksp632I     
KspI        MamI        MfeI        MluI        MroI        
MunI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       RcaI        RsrII       SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfuI        SmlI        SnaBI       SpeI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   62 enzymes) #
###################################

AclI          850  AACGTT               AA^CGTT
AcyI          265  GGCGCC               G[GA]^CG[CT]C
AflIII       1065  ACATGT               A^C[GA][CT]GT
AocI          511  CCTCAGG              CC^T[ACGT]AGG
AspHI         548  GAGCTC               G[AT]GC[AT]^C
BamHI         284  GGATCC               G^GATCC
BbeI          265  GGCGCC               GGCGC^C
BcgI          156  CGAGCGCGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI         1111  CTAG                 C^TAG
BlpI           45  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I       45  GCTGAGC              GC^T[ACGT]AGC
BsaAI         148  TACGTG               [CT]AC^GT[GA]
BsaHI         265  GGCGCC               G[GA]^CG[CT]C
BsiHKAI       548  GAGCTC               G[AT]GC[AT]^C
BsmBI         489  CGTCTC               CGTCTC(1/5)
BspLU11I     1065  ACATGT               A^CATGT
BsrBI          48  GAGCGG               CCGCTC(-3/-3)
BsrDI         612  GCAATG               GCAATG(2/0)
BssHII        159  GCGCGC               G^CGCGC
BstEII       1368  GGTGACC              G^GT[ACGT]ACC
BstPI        1368  GGTGACC              G^GT[ACGT]ACC
BstXI         818  CCACCAAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         284  GGATCC               [GA]^GATC[CT]
Bsu36I        511  CCTCAGG              CC^T[ACGT]AGG
CelII          45  GCTGAGC              GC^T[ACGT]AGC
DraII         253  GGGCCCC              [GA]G^G[ACGT]CC[CT]
DraIII       1363  CACATGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI          543  CGGCCG               C^GGCCG
EclXI         543  CGGCCG               C^GGCCG
Eco52I        543  CGGCCG               C^GGCCG
Eco81I        511  CCTCAGG              CC^T[ACGT]AGG
EcoO109I      253  GGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoO65I      1368  GGTGACC              G^GT[ACGT]ACC
EcoRI        1088  GAATTC               G^AATTC
EcoT14I      1309  CCATGG               C^C[AT][AT]GG
HgaI          179  GCGTC                GACGC(5/10)
Hin1I         265  GGCGCC               G[GA]^CG[CT]C
HincII        564  GTTGAC               GT[CT]^[GA]AC
HindII        564  GTTGAC               GT[CT]^[GA]AC
KasI          265  GGCGCC               G^GCGCC
MaeI         1111  CTAG                 C^TAG
MflI          284  GGATCC               [GA]^GATC[CT]
MseI         1051  TTAA                 T^TAA
MslI          963  CACACAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI          265  GGCGCC               GG^CGCC
NcoI         1309  CCATGG               C^CATGG
PflMI         144  CCAGTACGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I      850  AACGTT               AA^CGTT
PvuI          174  CGATCG               CGAT^CG
PvuII         940  CAGCTG               CAG^CTG
SacI          548  GAGCTC               GAGCT^C
SfoI          265  GGCGCC               GGC^GCC
SgrAI         261  CGCCGGCG             C[GA]^CCGG[CT]G
SmaI          250  CCCGGG               CCC^GGG
SphI         1024  GCATGC               GCATG^C
StyI         1309  CCATGG               C^C[AT][AT]GG
Tru9I        1051  TTAA                 T^TAA
Van91I        144  CCAGTACGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          699  CCAGGTCGCCTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         284  GGATCC               [GA]^GATC[CT]
XmaCI         250  CCCGGG               C^CCGGG
XmaI          250  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AcsI          641  AAATTT               [GA]^AATT[CT]
             1088  GAATTC              
AfaI          147  GTAC                 GT^AC
              933  GTAC                
ApaI          247  GGGCCC               GGGCC^C
              253  GGGCCC              
ApoI          641  AAATTT               [GA]^AATT[CT]
             1088  GAATTC              
Asp700I       596  GAAACAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1132  GAAATGATTC          
AvaI          204  CTCGGG               C^[CT]CG[GA]G
              250  CCCGGG              
BalI          687  TGGCCA               TGG^CCA
             1376  TGGCCA              
BbsI          181  GTCTTC               GAAGAC(2/6)
              583  GTCTTC              
BpmI          307  CTGGAG               CTGGAG(16/14)
             1291  CTGGAG              
BpuAI         181  GTCTTC               GAAGAC(2/6)
              583  GTCTTC              
BsaI          833  GAGACC               GGTCTC(1/5)
              534  GGTCTC              
BsgI         1220  CTGCAC               GTGCAG(16/14)
             1043  GTGCAG              
BsmFI         402  GGGAC                GGGAC(10/14)
             1126  GTCCC               
BsoBI         204  CTCGGG               C^[CT]CG[GA]G
              250  CCCGGG              
BspMI         935  ACCTGC               ACCTGC(4/8)
              531  GCAGGT              
BsrFI         101  GCCGGC               [GA]^CCGG[CT]
              262  GCCGGC              
Cfr10I        101  GCCGGC               [GA]^CCGG[CT]
              262  GCCGGC              
MluNI         687  TGGCCA               TGG^CCA
             1376  TGGCCA              
MscI          687  TGGCCA               TGG^CCA
             1376  TGGCCA              
MspA1I        940  CAGCTG               C[AC]G^C[GT]G
              981  CCGCTG              
NaeI          101  GCCGGC               GCC^GGC
              262  GCCGGC              
NgoMIV        101  GCCGGC               G^CCGGC
              262  GCCGGC              
PstI          529  CTGCAG               CTGCA^G
              937  CTGCAG              
RsaI          147  GTAC                 GT^AC
              933  GTAC                
SfcI          529  CTGCAG               C^T[GA][CT]AG
              937  CTGCAG              
TaqI          632  TCGA                 T^CGA
              683  TCGA                
TthHB8I       632  TCGA                 T^CGA
              683  TCGA                
XmnI          596  GAAACAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1132  GAAATGATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   65 enzymes) #
#########################################

AccII       AciI        AluI        AlwI        AvaII       
BanI        BanII       BbvI        BcnI        BmyI        
BsaJI       BsiEI       BsiYI       BslI        BsmAI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstUI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DsaI        EaeI        EcoRII      FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        MaeII       MaeIII      MboI        MboII       
MnlI        MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PleI        Sau3AI      Sau96I      ScrFI       SfaNI       
TfiI        TseI        Tsp45I      Tsp509I     TspRI       
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