Restriction map for pF1KB8670
 (Linear) (Six-base) MAPSORT of: KIBB8670.seq  Check: 7790  from: 1  to: 1392



KIBB8670.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:07  ..

AclI AA'CG_TT

Cuts at:      0     851    1392 
   Size:        851     541
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     180     570    1392 
   Size:        180     390     822

  Fragments arranged by size: 

                 822     390     180
 
AflIII A'CryG_T

Cuts at:      0    1065    1392 
   Size:       1065     327
 
AleI CACnn'nnGTG

Cuts at:      0     967    1392 
   Size:        967     425
 
ApaI G_GGCC'C

Cuts at:      0     251     257    1392 
   Size:        251       6    1135

  Fragments arranged by size: 

                1135     251       6
 
ApoI r'AATT_y

Cuts at:      0     641    1088    1392 
   Size:        641     447     304

  Fragments arranged by size: 

                 641     447     304
 
AvaI C'yCGr_G

Cuts at:      0     204     250    1392 
   Size:        204      46    1142

  Fragments arranged by size: 

                1142     204      46
 
BamHI G'GATC_C

Cuts at:      0     284    1392 
   Size:        284    1108
 
BanI G'GyrC_C

Cuts at:      0     132     234     265    1271    1392 
   Size:        132     102      31    1006     121

  Fragments arranged by size: 

                1006     132     121     102      31
 
BanII G_rGCy'C

Cuts at:      0      30     251     257     482     552    1392 
   Size:         30     221       6     225      70     840

  Fragments arranged by size: 

                 840     225     221      70      30       6
 
BbeI G_GCGC'C

Cuts at:      0     269    1392 
   Size:        269    1123
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     174     576    1392 
   Size:        174     402     816

  Fragments arranged by size: 

                 816     402     174
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     145     179    1392 
   Size:        145      34    1213

  Fragments arranged by size: 

                1213     145      34
 
BlpI GC'TnA_GC

Cuts at:      0      46    1392 
   Size:         46    1346
 
Bme1580I G_kGCm'C

Cuts at:      0      74     137     239     251     257     299     980    1392 
   Size:         74      63     102      12       6      42     681     412

  Fragments arranged by size: 

                 681     412     102      74      63      42      12       6
            
 
BmgBI CAC'GTC

Cuts at:      0    1344    1392 
   Size:       1344      48
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     328    1312    1392 
   Size:        328     984      80

  Fragments arranged by size: 

                 984     328      80
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     540     827    1392 
   Size:        540     287     565

  Fragments arranged by size: 

                 565     540     287
 
BsaAI yAC'GTr

Cuts at:      0     150    1392 
   Size:        150    1242
 
BsaHI Gr'CG_yC

Cuts at:      0     266    1392 
   Size:        266    1126
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     836     866    1285    1315    1392 
   Size:        836      30     419      30      77

  Fragments arranged by size: 

                 836     419      77      30      30
 
BseYI C'CCAG_C

Cuts at:      0      22      33      60     213     238     825    1392 
   Size:         22      11      27     153      25     587     567

  Fragments arranged by size: 

                 587     567     153      27      25      22      11
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1064    1205    1392 
   Size:       1064     141     187

  Fragments arranged by size: 

                1064     187     141
 
BsiEI CG_ry'CG

Cuts at:      0     177     274     546    1287    1392 
   Size:        177      97     272     741     105

  Fragments arranged by size: 

                 741     272     177     105      97
 
BsiHKAI G_wGCw'C

Cuts at:      0     552    1392 
   Size:        552     840
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     495    1392 
   Size:        495     897
 
Bsp1286I G_dGCh'C

Cuts at:      0      30      74     137     239     251     257     299     482 
   Size:         30      44      63     102      12       6      42     183

Cuts at:    482     552     980    1392 
   Size:         70     428     412

  Fragments arranged by size: 

                 428     412     183     102      70      63      44      42
                  30      12       6
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     522     944    1392 
   Size:        522     422     448

  Fragments arranged by size: 

                 522     448     422
 
BsrBI CCG'CTC

Cuts at:      0      50    1392 
   Size:         50    1342
 
BsrDI GCAATG_nn'

Cuts at:      0     619    1392 
   Size:        619     773
 
BsrFI r'CCGG_y

Cuts at:      0     101     262    1392 
   Size:        101     161    1130

  Fragments arranged by size: 

                1130     161     101
 
BssHII G'CGCG_C

Cuts at:      0     159    1392 
   Size:        159    1233
 
BstEII G'GTnAC_C

Cuts at:      0    1368    1392 
   Size:       1368      24
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     825    1392 
   Size:        825     567
 
BstYI r'GATC_y

Cuts at:      0     284    1392 
   Size:        284    1108
 
Bsu36I CC'TnA_GG

Cuts at:      0     512    1392 
   Size:        512     880
 
BtgI C'CryG_G

Cuts at:      0     137     666    1309    1392 
   Size:        137     529     643      83

  Fragments arranged by size: 

                 643     529     137      83
 
BtsI GCAGTG_nn'

Cuts at:      0     355    1052    1392 
   Size:        355     697     340

  Fragments arranged by size: 

                 697     355     340
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     810     845    1094    1129    1134    1169    1392 
   Size:        810      35     249      35       5      35     223

  Fragments arranged by size: 

                 810     249     223      35      35      35       5
 
DraIII CAC_nnn'GTG

Cuts at:      0    1368    1392 
   Size:       1368      24
 
EaeI y'GGCC_r

Cuts at:      0      83     543     687     887    1376    1392 
   Size:         83     460     144     200     489      16

  Fragments arranged by size: 

                 489     460     200     144      83      16
 
EagI C'GGCC_G

Cuts at:      0     543    1392 
   Size:        543     849
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     142     885    1392 
   Size:        142     743     507

  Fragments arranged by size: 

                 743     507     142
 
EcoICRI GAG'CTC

Cuts at:      0     550    1392 
   Size:        550     842
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     180     328     570    1312    1392 
   Size:        180     148     242     742      80

  Fragments arranged by size: 

                 742     242     180     148      80
 
EcoO109I rG'GnC_Cy

Cuts at:      0     254    1392 
   Size:        254    1138
 
EcoRI G'AATT_C

Cuts at:      0    1088    1392 
   Size:       1088     304
 
HaeII r_GCGC'y

Cuts at:      0       7      93     214     269    1392 
   Size:          7      86     121      55    1123

  Fragments arranged by size: 

                1123     121      86      55       7
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1190    1222    1392 
   Size:       1190      32     170

  Fragments arranged by size: 

                1190     170      32
 
HincII GTy'rAC

Cuts at:      0     566    1392 
   Size:        566     826
 
KasI G'GCGC_C

Cuts at:      0     265    1392 
   Size:        265    1127
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     455    1392 
   Size:        455     937
 
MscI TGG'CCA

Cuts at:      0     689    1378    1392 
   Size:        689     689      14

  Fragments arranged by size: 

                 689     689      14
 
MslI CAynn'nnrTG

Cuts at:      0     967    1392 
   Size:        967     425
 
MspA1I CmG'CkG

Cuts at:      0     942     983    1392 
   Size:        942      41     409

  Fragments arranged by size: 

                 942     409      41
 
NaeI GCC'GGC

Cuts at:      0     103     264    1392 
   Size:        103     161    1128

  Fragments arranged by size: 

                1128     161     103
 
NarI GG'CG_CC

Cuts at:      0     266    1392 
   Size:        266    1126
 
NcoI C'CATG_G

Cuts at:      0    1309    1392 
   Size:       1309      83
 
NgoMIV G'CCGG_C

Cuts at:      0     101     262    1392 
   Size:        101     161    1130

  Fragments arranged by size: 

                1130     161     101
 
NspI r_CATG'y

Cuts at:      0    1028    1069    1352    1392 
   Size:       1028      41     283      40

  Fragments arranged by size: 

                1028     283      41      40
 
PciI A'CATG_T

Cuts at:      0    1065    1392 
   Size:       1065     327
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     150    1392 
   Size:        150    1242
 
PfoI T'CCnGG_A

Cuts at:      0      94    1392 
   Size:         94    1298
 
PspOMI G'GGCC_C

Cuts at:      0     247     253    1392 
   Size:        247       6    1139

  Fragments arranged by size: 

                1139     247       6
 
PstI C_TGCA'G

Cuts at:      0     533     941    1392 
   Size:        533     408     451

  Fragments arranged by size: 

                 533     451     408
 
PvuI CG_AT'CG

Cuts at:      0     177    1392 
   Size:        177    1215
 
PvuII CAG'CTG

Cuts at:      0     942    1392 
   Size:        942     450
 
SacI G_AGCT'C

Cuts at:      0     552    1392 
   Size:        552     840
 
SfcI C'TryA_G

Cuts at:      0     529     937    1392 
   Size:        529     408     455

  Fragments arranged by size: 

                 529     455     408
 
SfoI GGC'GCC

Cuts at:      0     267    1392 
   Size:        267    1125
 
SgrAI Cr'CCGG_yG

Cuts at:      0     262    1392 
   Size:        262    1130
 
SmaI CCC'GGG

Cuts at:      0     252    1392 
   Size:        252    1140
 
SphI G_CATG'C

Cuts at:      0    1028    1392 
   Size:       1028     364
 
SrfI GCCC'GGGC

Cuts at:      0     252    1392 
   Size:        252    1140
 
StyI C'CwwG_G

Cuts at:      0    1309    1392 
   Size:       1309      83
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     817    1158    1273    1392 
   Size:        817     341     115     119

  Fragments arranged by size: 

                 817     341     119     115
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     611     643    1392 
   Size:        611      32     749

  Fragments arranged by size: 

                 749     611      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     706    1392 
   Size:        706     686
 
XmaI C'CCGG_G

Cuts at:      0     250    1392 
   Size:        250    1142
 
XmnI GAAnn'nnTTC

Cuts at:      0     600    1136    1392 
   Size:        600     536     256

  Fragments arranged by size: 

                 600     536     256
 

 Enzymes that do cut:

     AclI     AcuI   AflIII     AleI     ApaI     ApoI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI     BcgI     BlpI Bme1580I    BmgBI
     BpmI     BsaI    BsaAI    BsaHI    BsaXI    BseYI     BsgI    BsiEI
  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI   BssHII
   BstEII    BstXI    BstYI   Bsu36I     BtgI     BtsI    CspCI   DraIII
     EaeI     EagI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI    HaeII
    Hin4I   HincII     KasI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NspI     PciI    PflMI     PfoI   PspOMI
     PstI     PvuI    PvuII     SacI     SfcI     SfoI    SgrAI     SmaI
     SphI     SrfI     StyI    TaqII     TstI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII     AgeI     AhdI
     AlfI     AloI    AlwNI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BbvCI    BciVI     BclI    BfrBI     BglI    BglII     BmrI
     BmtI     BplI   Bpu10I    BpuEI    BsaBI    BsaWI    BseRI    BsiWI
     BsmI    BspEI    BspHI    BsrGI    BssSI   BstAPI    BstBI  BstZ17I
    BtgZI     ClaI     DraI     DrdI     EarI    EcoNI    EcoRV     FalI
     FseI     FspI    FspAI  HindIII     HpaI     KpnI     MfeI     MluI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI    RsrII
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SgfI     SmlI    SnaBI     SpeI     SspI     StuI     SwaI     TatI
  Tth111I     XbaI     XhoI     ZraI
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