Restriction map for pF1KB8667
##################################
# NO CUT ENZYMES (  147 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspHI       AspI        
AvaI        AviII       AvrII       BalI        BanI        
BbeI        BbrPI       BbvCI       BciVI       BclI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsiHKAI     BsiWI       BsmFI       
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrFI       BsrGI       BssHII      BssSI       
BstAPI      BstBI       BstEII      BstF5I      BstPI       
Bsu36I      CelII       CfoI        Cfr10I      ClaI        
CpoI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRV       EcoT14I     FbaI        FokI        
FseI        FspI        HaeII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MamI        
MfeI        MluI        MluNI       MroI        MscI        
MunI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NspI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PleI        PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       StuI        
StyI        SwaI        Tth111I     XbaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccI          436  GTATAC               GT^[AC][GT]AC
AfaI           38  GTAC                 GT^AC
AlwNI         178  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI         1224  GGGCCC               GGGCC^C
Asp700I      1125  GAAGCTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbsI          869  GTCTTC               GAAGAC(2/6)
BcgI         1053  GCAGATTCCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI          194  CCGGG                CC^[GC]GG
BpmI         1157  CTCCAG               CTGGAG(16/14)
BpuAI         869  GTCTTC               GAAGAC(2/6)
BsgI          812  GTGCAG               GTGCAG(16/14)
BsiEI         157  CGACCG               CG[GA][CT]^CG
BsmAI         797  GAGAC                GTCTC(1/5)
BsmBI         797  GAGACG               CGTCTC(1/5)
BsmI          566  GAATGC               GAATGC(1/-1)
BspMI          16  ACCTGC               ACCTGC(4/8)
BsrBI        1182  CCGCTC               CCGCTC(-3/-3)
BsrDI         144  CATTGC               GCAATG(2/0)
Bst1107I      436  GTATAC               GTA^TAC
BstXI        1214  CCAAGCGGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I       436  GTATAC               GTA^TAC
DraI         1111  TTTAAA               TTT^AAA
DraII          96  GGGTCCT              [GA]G^G[ACGT]CC[CT]
DsaI          864  CCGTGG               C^C[GA][CT]GG
EaeI         1244  TGGCCG               [CT]^GGCC[GA]
EarI          292  CTCTTC               CTCTTC(1/4)
EcoO109I       96  GGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoRI         767  GAATTC               G^AATTC
EcoT22I       554  ATGCAT               ATGCA^T
FauI          148  GCGGG                CCCGC(4/6)
Ksp632I       292  CTCTTC               CTCTTC(1/4)
MaeII          74  ACGT                 A^CGT
NciI          194  CCGGG                CC^[GC]GG
NsiI          554  ATGCAT               ATGCA^T
PflMI        1228  CCAGGCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI          96  GGGTCCT              [GA]G^G[AT]CC[CT]
PshAI         134  GACTGGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI           47  CTGCAG               CTGCA^G
PvuII         178  CAGCTG               CAG^CTG
RsaI           38  GTAC                 GT^AC
SapI          291  GCTCTTC              GCTCTTC(1/4)
ScaI           37  AGTACT               AGT^ACT
SfcI           47  CTGCAG               C^T[GA][CT]AG
SmlI         1185  CTCAAG               C^T[CT][GA]AG
TaqI          132  TCGA                 T^CGA
TthHB8I       132  TCGA                 T^CGA
Van91I       1228  CCAGGCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          213  CCAGCTCTTAGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         1125  GAAGCTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AccII          33  CGCG                 CG^CG
              155  CGCG                
AcsI          421  GAATTT               [GA]^AATT[CT]
              767  GAATTC              
ApoI          421  GAATTT               [GA]^AATT[CT]
              767  GAATTC              
AvaII          97  GGTCC                G^G[AT]CC
              517  GGACC               
BamHI         360  GGATCC               G^GATCC
              724  GGATCC              
BanII         196  GGGCTC               G[GA]GC[CT]^C
             1224  GGGCCC              
BbvI          180  GCTGC                GCAGC(8/12)
              678  GCTGC               
BfaI          101  CTAG                 C^TAG
              208  CTAG                
BseRI          90  GAGGAG               GAGGAG(10/8)
              259  GAGGAG              
BstUI          33  CGCG                 CG^CG
              155  CGCG                
HapII         194  CCGG                 C^CGG
             1247  CCGG                
HpaII         194  CCGG                 C^CGG
             1247  CCGG                
MaeI          101  CTAG                 C^TAG
              208  CTAG                
MspA1I        123  CAGCGG               C[AC]G^C[GT]G
              178  CAGCTG              
MspI          194  CCGG                 C^CGG
             1247  CCGG                
MvnI           33  CGCG                 CG^CG
              155  CGCG                
SspI          274  AATATT               AAT^ATT
              463  AATATT              
TseI          180  GCTGC                G^C[AT]GC
              678  GCTGC               


#########################################
# 3 OR MORE CUT ENZYMES (   44 enzymes) #
#########################################

AciI        AluI        AlwI        BmyI        BsaJI       
BsiYI       BslI        Bsp1286I    BsrI        BssKI       
BstNI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       EcoRII      Fnu4HI      HaeIII      HinfI       
HphI        ItaI        MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       Sau3AI      
Sau96I      ScrFI       SfaNI       TfiI        Tru9I       
Tsp45I      Tsp509I     TspRI       XhoII       
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