Restriction map for pF1KB8656
##################################
# NO CUT ENZYMES (  138 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AhdI        Alw44I      AlwNI       
Aor51HI     ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaHI       
BsaI        BseAI       BsiEI       BsiWI       BsmBI       
BsoBI       Bsp1407I    BspDI       BspEI       BsrBI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstEII      BstNI       BstPI       BstUI       BstXI       
BstZ17I     CelII       ClaI        CpoI        DrdI        
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoO65I     EcoRII      EcoRV       
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HgaI        Hin1I       HpaI        KasI        
KpnI        KspI        MluI        MluNI       MroI        
MscI        MvaI        MvnI        NaeI        NarI        
NcoI        NgoMIV      NheI        NotI        NruI        
NsiI        PacI        PaeR7I      PflFI       PflMI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SgrAI       SmaI        SnaBI       SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tth111I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AciI          289  CCGC                 CCGC(-3/-1)
AflIII        305  ACATGT               A^C[GA][CT]GT
AgeI          536  ACCGGT               A^CCGGT
AlwI          573  GGATC                GGATC(4/5)
AocI          278  CCTCAGG              CC^T[ACGT]AGG
ApaI          469  GGGCCC               GGGCC^C
BanI         1332  GGCACC               G^G[CT][GA]CC
BfaI          352  CTAG                 C^TAG
BsaWI         536  ACCGGT               [AT]^CCGG[AT]
BseRI         626  CTCCTC               GAGGAG(10/8)
BsgI         1164  CTGCAC               GTGCAG(16/14)
BsmFI         766  GGGAC                GGGAC(10/14)
BspHI        1635  TCATGA               T^CATGA
BspLU11I      305  ACATGT               A^CATGT
BsrFI         536  ACCGGT               [GA]^CCGG[CT]
BstBI         555  TTCGAA               TT^CGAA
BstYI         573  GGATCT               [GA]^GATC[CT]
Bsu36I        278  CCTCAGG              CC^T[ACGT]AGG
CfoI         1128  GCGC                 GCG^C
Cfr10I        536  ACCGGT               [GA]^CCGG[CT]
DraII         469  GGGCCCC              [GA]G^G[ACGT]CC[CT]
Eco81I        278  CCTCAGG              CC^T[ACGT]AGG
EcoO109I      469  GGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoRI        1239  GAATTC               G^AATTC
EcoT14I       928  CCAAGG               C^C[AT][AT]GG
HhaI         1128  GCGC                 GCG^C
HinP1I       1128  GCGC                 G^CGC
HincII       1295  GTCAAC               GT[CT]^[GA]AC
HindII       1295  GTCAAC               GT[CT]^[GA]AC
HindIII       747  AAGCTT               A^AGCTT
MaeI          352  CTAG                 C^TAG
MaeII        1293  ACGT                 A^CGT
MfeI          613  CAATTG               C^AATTG
MflI          573  GGATCT               [GA]^GATC[CT]
MslI         1665  CATGGAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        770  CAGCTG               C[AC]G^C[GT]G
MunI          613  CAATTG               C^AATTG
NdeI          833  CATATG               CA^TATG
NspV          555  TTCGAA               TT^CGAA
PinAI         536  ACCGGT               A^CCGGT
PstI           95  CTGCAG               CTGCA^G
PvuII         770  CAGCTG               CAG^CTG
RcaI         1635  TCATGA               T^CATGA
SacI          483  GAGCTC               GAGCT^C
SapI          910  GCTCTTC              GCTCTTC(1/4)
SfcI           95  CTGCAG               C^T[GA][CT]AG
SfuI          555  TTCGAA               TT^CGAA
SmlI           75  CTTGAG               C^T[CT][GA]AG
SpeI          351  ACTAGT               A^CTAGT
StyI          928  CCAAGG               C^C[AT][AT]GG
TfiI          793  GATTC                G^A[AT]TC
XhoII         573  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AfaI          128  GTAC                 GT^AC
              355  GTAC                
AspHI          78  GAGCAC               G[AT]GC[AT]^C
              483  GAGCTC              
AvaII          49  GGTCC                G^G[AT]CC
              415  GGTCC               
BcnI          467  CCGGG                CC^[GC]GG
              473  CCCGG               
BsaBI         329  GATTTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1465  GATGAAAATC          
BsaJI         472  CCCCGG               C^C[ACGT][ACGT]GG
              928  CCAAGG              
BsiHKAI        78  GAGCAC               G[AT]GC[AT]^C
              483  GAGCTC              
BsmAI         369  GAGAC                GTCTC(1/5)
              775  GAGAC               
BsmI          918  GCATTC               GAATGC(1/-1)
             1401  GCATTC              
BspMI         684  ACCTGC               ACCTGC(4/8)
             1133  ACCTGC              
BsrDI         220  GCAATG               GCAATG(2/0)
              934  GCAATG              
BssKI         467  CCGGG                ^CC[ACGT]GG
              473  CCCGG               
Cac8I         225  GCGAGC               GC[ACGT]^[ACGT]GC
              813  GCAGGC              
DraIII        185  CACAATGTG            CAC[ACGT][ACGT][ACGT]^GTG
              563  CACCCAGTG           
HaeIII        470  GGCC                 GG^CC
              509  GGCC                
MamI          329  GATTTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1465  GATGAAAATC          
MwoI          220  GCAATGCGAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
             1128  GCGCCACCTGC         
NciI          467  CCGGG                CC^[GC]GG
              473  CCCGG               
NspI          305  ACATGT               [GA]CATG^[CT]
             1140  GCATGT              
PleI          880  GACTC                GAGTC(4/5)
               17  GAGTC               
RsaI          128  GTAC                 GT^AC
              355  GTAC                
ScrFI         467  CCGGG                CC^[ACGT]GG
              473  CCCGG               
TaqI          556  TCGA                 T^CGA
             1082  TCGA                
TthHB8I       556  TCGA                 T^CGA
             1082  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   44 enzymes) #
#########################################

AccI        AcsI        AluI        ApoI        BanII       
BbvI        BmyI        BpmI        BsiYI       BslI        
Bsp1286I    BsrI        BstF5I      Cfr13I      DdeI        
DpnII       DraI        EarI        EcoNI       Fnu4HI      
FokI        HapII       HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeIII      MboI        MboII       
MnlI        MseI        MspI        NdeII       NlaIII      
NlaIV       Sau3AI      Sau96I      SfaNI       Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       
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