Restriction map for pF1KB8641
 (Linear) (Six-base) MAPSORT of: KIBB8641.seq  Check: 6849  from: 1  to: 2577



KIBB8641.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     681    2390    2577 
   Size:        681    1709     187

  Fragments arranged by size: 

                1709     681     187
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     347     566     893    2383    2577 
   Size:        347     219     327    1490     194

  Fragments arranged by size: 

                1490     347     327     219     194
 
AflIII A'CryG_T

Cuts at:      0    2297    2577 
   Size:       2297     280
 
AleI CACnn'nnGTG

Cuts at:      0    1534    2021    2577 
   Size:       1534     487     556

  Fragments arranged by size: 

                1534     556     487
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1238    2577 
   Size:       1238    1339
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2269    2397    2577 
   Size:       2269     128     180

  Fragments arranged by size: 

                2269     180     128
 
ApoI r'AATT_y

Cuts at:      0      49     349    2577 
   Size:         49     300    2228

  Fragments arranged by size: 

                2228     300      49
 
AseI AT'TA_AT

Cuts at:      0    1351    2577 
   Size:       1351    1226
 
AvaI C'yCGr_G

Cuts at:      0    1187    1943    2036    2577 
   Size:       1187     756      93     541

  Fragments arranged by size: 

                1187     756     541      93
 
BanI G'GyrC_C

Cuts at:      0     516     669    1397    2577 
   Size:        516     153     728    1180

  Fragments arranged by size: 

                1180     728     516     153
 
BanII G_rGCy'C

Cuts at:      0    1454    1817    2577 
   Size:       1454     363     760

  Fragments arranged by size: 

                1454     760     363
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     538    1916    2300    2524    2577 
   Size:        538    1378     384     224      53

  Fragments arranged by size: 

                1378     538     384     224      53
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1766    2435    2577 
   Size:       1766     669     142

  Fragments arranged by size: 

                1766     669     142
 
BclI T'GATC_A

Cuts at:      0     555    1031    2087    2577 
   Size:        555     476    1056     490

  Fragments arranged by size: 

                1056     555     490     476
 
BfrBI ATG'CAT

Cuts at:      0     889    2577 
   Size:        889    1688
 
BlpI GC'TnA_GC

Cuts at:      0    1448    2577 
   Size:       1448    1129
 
Bme1580I G_kGCm'C

Cuts at:      0     521    2577 
   Size:        521    2056
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     923    2577 
   Size:        923    1654
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     180     394     842     983    1316    1909    2296    2577 
   Size:        180     214     448     141     333     593     387     281

  Fragments arranged by size: 

                 593     448     387     333     281     214     180     141
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     368    1393    2577 
   Size:        368    1025    1184

  Fragments arranged by size: 

                1184    1025     368
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2101    2577 
   Size:       2101     476
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     180     820    1687    2577 
   Size:        180     640     867     890

  Fragments arranged by size: 

                 890     867     640     180
 
BsaAI yAC'GTr

Cuts at:      0    1996    2577 
   Size:       1996     581
 
BsaWI w'CCGG_w

Cuts at:      0     408    1421    2577 
   Size:        408    1013    1156

  Fragments arranged by size: 

                1156    1013     408
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     256     286     956     986    1592    1622    1774    1804 
   Size:        256      30     670      30     606      30     152      30

Cuts at:   1804    2577 
   Size:        773

  Fragments arranged by size: 

                 773     670     606     256     152      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     268     271     274     277     785    1084    1807    2577 
   Size:        268       3       3       3     508     299     723     770

  Fragments arranged by size: 

                 770     723     508     299     268       3       3       3
            
 
BseYI C'CCAG_C

Cuts at:      0     415    2390    2577 
   Size:        415    1975     187

  Fragments arranged by size: 

                1975     415     187
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     623    2170    2224    2577 
   Size:        623    1547      54     353

  Fragments arranged by size: 

                1547     623     353      54
 
BsiHKAI G_wGCw'C

Cuts at:      0     505     618    1454    2577 
   Size:        505     113     836    1123

  Fragments arranged by size: 

                1123     836     505     113
 
BsmI GAATG_Cn'

Cuts at:      0     789    2577 
   Size:        789    1788
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1675    2577 
   Size:       1675     902
 
Bsp1286I G_dGCh'C

Cuts at:      0     505     521     618    1454    1817    2577 
   Size:        505      16      97     836     363     760

  Fragments arranged by size: 

                 836     760     505     363      97      16
 
BspEI T'CCGG_A

Cuts at:      0     408    2577 
   Size:        408    2169
 
BspHI T'CATG_A

Cuts at:      0     552    2577 
   Size:        552    2025
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     681    2390    2577 
   Size:        681    1709     187

  Fragments arranged by size: 

                1709     681     187
 
BsrDI GCAATG_nn'

Cuts at:      0     889    2577 
   Size:        889    1688
 
BsrFI r'CCGG_y

Cuts at:      0     603    1337    2577 
   Size:        603     734    1240

  Fragments arranged by size: 

                1240     734     603
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1296    2269    2577 
   Size:       1296     973     308

  Fragments arranged by size: 

                1296     973     308
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1584    2023    2577 
   Size:       1584     439     554

  Fragments arranged by size: 

                1584     554     439
 
BstYI r'GATC_y

Cuts at:      0     363    1700    2577 
   Size:        363    1337     877

  Fragments arranged by size: 

                1337     877     363
 
Bsu36I CC'TnA_GG

Cuts at:      0    2078    2577 
   Size:       2078     499
 
BtgI C'CryG_G

Cuts at:      0    1673    2577 
   Size:       1673     904
 
BtsI GCAGTG_nn'

Cuts at:      0     503     775     922    2577 
   Size:        503     272     147    1655

  Fragments arranged by size: 

                1655     503     272     147
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1916    1951    2577 
   Size:       1916      35     626

  Fragments arranged by size: 

                1916     626      35
 
EaeI y'GGCC_r

Cuts at:      0    1196    1229    2577 
   Size:       1196      33    1348

  Fragments arranged by size: 

                1348    1196      33
 
EarI CTCTTCn'nnn_

Cuts at:      0     208     245     772    2577 
   Size:        208      37     527    1805

  Fragments arranged by size: 

                1805     527     208      37
 
EcoICRI GAG'CTC

Cuts at:      0    1452    2577 
   Size:       1452    1125
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     180     347     394     566     842     893     983    1316 
   Size:        180     167      47     172     276      51      90     333

Cuts at:   1316    1909    2296    2383    2577 
   Size:        593     387      87     194

  Fragments arranged by size: 

                 593     387     333     276     194     180     172     167
                  90      87      51      47
 
EcoO109I rG'GnC_Cy

Cuts at:      0     585    1018    1989    2577 
   Size:        585     433     971     588

  Fragments arranged by size: 

                 971     588     585     433
 
EcoRV GAT'ATC

Cuts at:      0    1833    2133    2577 
   Size:       1833     300     444

  Fragments arranged by size: 

                1833     444     300
 
HaeII r_GCGC'y

Cuts at:      0    1133    2577 
   Size:       1133    1444
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       4      36     256     288     412     444     841     873 
   Size:          4      32     220      32     124      32     397      32

Cuts at:    873     956     988    1086    1118    1170    1202    1740    1772 
   Size:         83      32      98      32      52      32     538      32

Cuts at:   1772    2577 
   Size:        805

  Fragments arranged by size: 

                 805     538     397     220     124      98      83      52
                  32      32      32      32      32      32      32      32
                   4
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     467    1097    2317    2577 
   Size:        467     630    1220     260

  Fragments arranged by size: 

                1220     630     467     260
 
MscI TGG'CCA

Cuts at:      0    1231    2577 
   Size:       1231    1346
 
MslI CAynn'nnrTG

Cuts at:      0     748    1220    1534    2021    2167    2577 
   Size:        748     472     314     487     146     410

  Fragments arranged by size: 

                 748     487     472     410     314     146
 
MspA1I CmG'CkG

Cuts at:      0    1461    2269    2577 
   Size:       1461     808     308

  Fragments arranged by size: 

                1461     808     308
 
NaeI GCC'GGC

Cuts at:      0    1339    2577 
   Size:       1339    1238
 
NgoMIV G'CCGG_C

Cuts at:      0    1337    2577 
   Size:       1337    1240
 
NsiI A_TGCA'T

Cuts at:      0     891    2577 
   Size:        891    1686
 
NspI r_CATG'y

Cuts at:      0    1855    1975    2301    2577 
   Size:       1855     120     326     276

  Fragments arranged by size: 

                1855     326     276     120
 
PacI TTA_AT'TAA

Cuts at:      0    1355    2577 
   Size:       1355    1222
 
PasI CC'CwG_GG

Cuts at:      0     381    2577 
   Size:        381    2196
 
PciI A'CATG_T

Cuts at:      0    2297    2577 
   Size:       2297     280
 
PpuMI rG'GwC_Cy

Cuts at:      0     585    1989    2577 
   Size:        585    1404     588

  Fragments arranged by size: 

                1404     588     585
 
PspXI vC'TCGA_Gb

Cuts at:      0    2036    2577 
   Size:       2036     541
 
PvuII CAG'CTG

Cuts at:      0    1461    2269    2577 
   Size:       1461     808     308

  Fragments arranged by size: 

                1461     808     308
 
SacI G_AGCT'C

Cuts at:      0    1454    2577 
   Size:       1454    1123
 
SexAI A'CCwGG_T

Cuts at:      0     437    1985    2577 
   Size:        437    1548     592

  Fragments arranged by size: 

                1548     592     437
 
SmlI C'TyrA_G

Cuts at:      0    1187    2036    2116    2577 
   Size:       1187     849      80     461

  Fragments arranged by size: 

                1187     849     461      80
 
SphI G_CATG'C

Cuts at:      0    1855    2577 
   Size:       1855     722
 
StuI AGG'CCT

Cuts at:      0    2054    2577 
   Size:       2054     523
 
StyI C'CwwG_G

Cuts at:      0     512     665     699    1643    2577 
   Size:        512     153      34     944     934

  Fragments arranged by size: 

                 944     934     512     153      34
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     234     657    2577 
   Size:        234     423    1920

  Fragments arranged by size: 

                1920     423     234
 
TatI w'GTAC_w

Cuts at:      0    1736    2563    2577 
   Size:       1736     827      14

  Fragments arranged by size: 

                1736     827      14
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     662     694    2371    2403    2577 
   Size:        662      32    1677      32     174

  Fragments arranged by size: 

                1677     662     174      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1191    1340    1530    2577 
   Size:       1191     149     190    1047

  Fragments arranged by size: 

                1191    1047     190     149
 
XhoI C'TCGA_G

Cuts at:      0    1187    2036    2577 
   Size:       1187     849     541

  Fragments arranged by size: 

                1187     849     541
 
XmnI GAAnn'nnTTC

Cuts at:      0    1726    2577 
   Size:       1726     851
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AleI     AlfI    AlwNI     ApoI     AseI
     AvaI     BanI    BanII     BbsI    BciVI     BclI    BfrBI     BlpI
 Bme1580I     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaWI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrDI    BsrFI   BstAPI    BstXI    BstYI
   Bsu36I     BtgI     BtsI    CspCI     EaeI     EarI  EcoICRI  Eco57MI
 EcoO109I    EcoRV    HaeII    Hin4I     MmeI     MscI     MslI   MspA1I
     NaeI   NgoMIV     NsiI     NspI     PacI     PasI     PciI    PpuMI
    PspXI    PvuII     SacI    SexAI     SmlI     SphI     StuI     StyI
    TaqII     TatI     TstI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AloI     ApaI    ApaLI     AscI    AsiSI    AvrII     BaeI    BamHI
     BbeI    BbvCI     BcgI     BglI    BglII    BmgBI     BmtI     BplI
    BsaBI    BsaHI    BsiEI    BsiWI    BsrBI    BsrGI   BssHII    BssSI
    BstBI   BstEII  BstZ17I    BtgZI     ClaI     DraI   DraIII     DrdI
     EagI     EciI    EcoNI    EcoRI     FalI     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     NarI
     NcoI     NdeI     NheI     NotI     NruI    PflMI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI     PsrI     PstI     PvuI
    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI
     SspI     SwaI  Tth111I     XbaI     XmaI     ZraI
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