Restriction map for pF1KB8638
##################################
# NO CUT ENZYMES (  154 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AfaI        AflII       AgeI        
AhdI        Alw44I      AlwI        AocI        ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BamHI       BanI        BanII       
BbeI        BbrPI       BbsI        BciVI       BclI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BseAI       BsiEI       BsiHKAI     BsiWI       
BsmBI       BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspMI       BsrDI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstYI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraI        DrdI        
EagI        Eam1105I    EclXI       Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRV       EcoT22I     FauI        
FbaI        FseI        FspI        HgaI        Hin1I       
HpaI        KasI        KpnI        KspI        MaeI        
MaeII       MamI        MfeI        MflI        MluI        
MroI        MunI        MvnI        NarI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tth111I     XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AciI         1059  CCGC                 CCGC(-3/-1)
AflIII        101  ACATGT               A^C[GA][CT]GT
AlwNI         824  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI       848  AGCGCT               AGC^GCT
BalI          513  TGGCCA               TGG^CCA
BbvCI        1048  GCTGAGG              CCTCAGC(-5/-2)
BcgI           31  GCAGAAATGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BmyI         1080  GTGCCC               G[AGT]GC[ACT]^C
BpmI          844  CTGGAG               CTGGAG(16/14)
BsaI         1000  GAGACC               GGTCTC(1/5)
BsaWI         666  TCCGGT               [AT]^CCGG[AT]
BseRI         700  GAGGAG               GAGGAG(10/8)
BsgI          938  GTGCAG               GTGCAG(16/14)
BsmAI        1000  GAGAC                GTCTC(1/5)
BsmFI        1092  GGGAC                GGGAC(10/14)
BsmI          321  GAATGC               GAATGC(1/-1)
Bsp1286I     1080  GTGCCC               G[AGT]GC[ACT]^C
BspLU11I      101  ACATGT               A^CATGT
BsrBI        1059  CCGCTC               CCGCTC(-3/-3)
CfoI          849  GCGC                 GCG^C
DraII        1092  GGGACCC              [GA]G^G[ACGT]CC[CT]
DraIII       1039  CACTTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          893  CCGTGG               C^C[GA][CT]GG
EarI          469  CTCTTC               CTCTTC(1/4)
Eco47III      848  AGCGCT               AGC^GCT
EcoO109I     1092  GGGACCC              [GA]G^G[ACGT]CC[CT]
EcoT14I       326  CCAAGG               C^C[AT][AT]GG
HaeII         848  AGCGCT               [GA]GCGC^[CT]
HhaI          849  GCGC                 GCG^C
HinP1I        849  GCGC                 G^CGC
HindIII       460  AAGCTT               A^AGCTT
Ksp632I       469  CTCTTC               CTCTTC(1/4)
MluNI         513  TGGCCA               TGG^CCA
MscI          513  TGGCCA               TGG^CCA
MslI          525  CATGAACATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI          101  ACATGT               [GA]CATG^[CT]
PflMI        1038  CCACTTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          354  GAGTC                GAGTC(4/5)
PpuMI        1092  GGGACCC              [GA]G^G[AT]CC[CT]
SfaNI         871  GATGC                GCATC(5/9)
StyI          326  CCAAGG               C^C[AT][AT]GG
Van91I       1038  CCACTTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AvaII         107  GGACC                G^G[AT]CC
             1093  GGACC               
BcnI          132  CCGGG                CC^[GC]GG
              590  CCCGG               
BsrFI         551  GCCGGC               [GA]^CCGG[CT]
              619  GCCGGC              
BsrI          267  ACTGG                ACTGG(1/-1)
              389  ACTGG               
BstXI         341  CCAAAGCTATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              524  CCATGAACATGG        
Cfr10I        551  GCCGGC               [GA]^CCGG[CT]
              619  GCCGGC              
DpnII          78  GATC                 ^GATC
              943  GATC                
EcoRI         585  GAATTC               G^AATTC
              628  GAATTC              
HincII        356  GTCAAC               GT[CT]^[GA]AC
              502  GTCAAC              
HindII        356  GTCAAC               GT[CT]^[GA]AC
              502  GTCAAC              
HphI          145  GGTGA                GGTGA(8/7)
              480  GGTGA               
MboI           78  GATC                 ^GATC
              943  GATC                
MspA1I        273  CAGCTG               C[AC]G^C[GT]G
              824  CAGCTG              
MwoI          637  GCTCACAAGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              646  GCTATCTTAGC         
NaeI          551  GCCGGC               GCC^GGC
              619  GCCGGC              
NciI          132  CCGGG                CC^[GC]GG
              590  CCCGG               
NdeII          78  GATC                 ^GATC
              943  GATC                
NgoMIV        551  GCCGGC               G^CCGGC
              619  GCCGGC              
PstI          879  CTGCAG               CTGCA^G
              905  CTGCAG              
PvuII         273  CAGCTG               CAG^CTG
              824  CAGCTG              
Sau3AI         78  GATC                 ^GATC
              943  GATC                
SfcI          879  CTGCAG               C^T[GA][CT]AG
              905  CTGCAG              
Tsp45I        252  GTCAC                ^GT[GC]AC
             1029  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   39 enzymes) #
#########################################

AcsI        AluI        ApoI        BbvI        BsaJI       
BsiYI       BslI        BssKI       BstF5I      BstNI       
Cac8I       Cfr13I      DdeI        EaeI        EcoRII      
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       ItaI        MaeIII      MboII       MnlI        
MseI        MspI        MvaI        NlaIII      NlaIV       
Sau96I      ScrFI       TaqI        TfiI        Tru9I       
TseI        Tsp509I     TspRI       TthHB8I     
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com