Restriction map for pF1KB8638
 (Linear) (Six-base) MAPSORT of: KIBB8638.seq  Check: 2973  from: 1  to: 1122



KIBB8638.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:07  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     764    1055    1122 
   Size:        764     291      67

  Fragments arranged by size: 

                 764     291      67
 
AfeI AGC'GCT

Cuts at:      0     850    1122 
   Size:        850     272
 
AflIII A'CryG_T

Cuts at:      0     101    1122 
   Size:        101    1021
 
AlwNI CAG_nnn'CTG

Cuts at:      0     829    1122 
   Size:        829     293
 
ApoI r'AATT_y

Cuts at:      0      91     301     307     403     585     628     911    1122 
   Size:         91     210       6      96     182      43     283     211

  Fragments arranged by size: 

                 283     211     210     182      96      91      43       6
            
 
BbvCI CC'TCA_GC

Cuts at:      0    1049    1122 
   Size:       1049      73
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      20      54    1122 
   Size:         20      34    1068

  Fragments arranged by size: 

                1068      34      20
 
Bme1580I G_kGCm'C

Cuts at:      0    1084    1122 
   Size:       1084      38
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     399    1122 
   Size:        399     723
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     865    1122 
   Size:        865     257
 
Bpu10I CC'TnA_GC

Cuts at:      0    1049    1122 
   Size:       1049      73
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     994    1122 
   Size:        994     128
 
BsaWI w'CCGG_w

Cuts at:      0     666    1122 
   Size:        666     456
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     715    1122 
   Size:        715     407
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     959    1122 
   Size:        959     163
 
BsmI GAATG_Cn'

Cuts at:      0     327    1122 
   Size:        327     795
 
Bsp1286I G_dGCh'C

Cuts at:      0    1084    1122 
   Size:       1084      38
 
BsrBI CCG'CTC

Cuts at:      0    1061    1122 
   Size:       1061      61
 
BsrFI r'CCGG_y

Cuts at:      0     551     619    1122 
   Size:        551      68     503

  Fragments arranged by size: 

                 551     503      68
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     348     531    1122 
   Size:        348     183     591

  Fragments arranged by size: 

                 591     348     183
 
BtgI C'CryG_G

Cuts at:      0     893    1122 
   Size:        893     229
 
BtsI GCAGTG_nn'

Cuts at:      0     968    1122 
   Size:        968     154
 
DraIII CAC_nnn'GTG

Cuts at:      0    1044    1122 
   Size:       1044      78
 
EaeI y'GGCC_r

Cuts at:      0     513     621     923    1122 
   Size:        513     108     302     199

  Fragments arranged by size: 

                 513     302     199     108
 
EarI CTCTTCn'nnn_

Cuts at:      0     475    1122 
   Size:        475     647
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     764     865    1055    1122 
   Size:        764     101     190      67

  Fragments arranged by size: 

                 764     190     101      67
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1093    1122 
   Size:       1093      29
 
EcoRI G'AATT_C

Cuts at:      0     585     628    1122 
   Size:        585      43     494

  Fragments arranged by size: 

                 585     494      43
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     666    1122 
   Size:        666     456
 
HaeII r_GCGC'y

Cuts at:      0     852    1122 
   Size:        852     270
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     235     267     710     742    1122 
   Size:        235      32     443      32     380

  Fragments arranged by size: 

                 443     380     235      32      32
 
HincII GTy'rAC

Cuts at:      0     358     504    1122 
   Size:        358     146     618

  Fragments arranged by size: 

                 618     358     146
 
HindIII A'AGCT_T

Cuts at:      0     460    1122 
   Size:        460     662
 
MscI TGG'CCA

Cuts at:      0     515    1122 
   Size:        515     607
 
MslI CAynn'nnrTG

Cuts at:      0     529    1122 
   Size:        529     593
 
MspA1I CmG'CkG

Cuts at:      0     275     826    1122 
   Size:        275     551     296

  Fragments arranged by size: 

                 551     296     275
 
NaeI GCC'GGC

Cuts at:      0     553     621    1122 
   Size:        553      68     501

  Fragments arranged by size: 

                 553     501      68
 
NgoMIV G'CCGG_C

Cuts at:      0     551     619    1122 
   Size:        551      68     503

  Fragments arranged by size: 

                 551     503      68
 
NspI r_CATG'y

Cuts at:      0     105    1122 
   Size:        105    1017
 
PciI A'CATG_T

Cuts at:      0     101    1122 
   Size:        101    1021
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1044    1122 
   Size:       1044      78
 
PpuMI rG'GwC_Cy

Cuts at:      0    1093    1122 
   Size:       1093      29
 
PstI C_TGCA'G

Cuts at:      0     883     909    1122 
   Size:        883      26     213

  Fragments arranged by size: 

                 883     213      26
 
PvuII CAG'CTG

Cuts at:      0     275     826    1122 
   Size:        275     551     296

  Fragments arranged by size: 

                 551     296     275
 
SanDI GG'GwC_CC

Cuts at:      0    1093    1122 
   Size:       1093      29
 
SfcI C'TryA_G

Cuts at:      0     879     905    1122 
   Size:        879      26     217

  Fragments arranged by size: 

                 879     217      26
 
StyI C'CwwG_G

Cuts at:      0     326    1122 
   Size:        326     796
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     133     998    1122 
   Size:        133     865     124

  Fragments arranged by size: 

                 865     133     124
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     748     780    1122 
   Size:        748      32     342

  Fragments arranged by size: 

                 748     342      32
 

 Enzymes that do cut:

     AcuI     AfeI   AflIII    AlwNI     ApoI    BbvCI     BcgI Bme1580I
     BmrI     BpmI   Bpu10I     BsaI    BsaWI    BseRI     BsgI     BsmI
 Bsp1286I    BsrBI    BsrFI    BstXI     BtgI     BtsI   DraIII     EaeI
     EarI  Eco57MI EcoO109I    EcoRI     FalI    HaeII    Hin4I   HincII
  HindIII     MscI     MslI   MspA1I     NaeI   NgoMIV     NspI     PciI
    PflMI    PpuMI     PstI    PvuII    SanDI     SfcI     StyI    TaqII
     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI
     BplI    BpuEI    BsaAI    BsaBI    BsaHI    BsaXI    BseYI    BsiEI
  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrDI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI     DraI     DrdI     EagI     EciI  EcoICRI
    EcoNI    EcoRV     FseI     FspI    FspAI     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     NarI     NcoI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PasI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI     TatI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI
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