Restriction map for pF1KB8636
##################################
# NO CUT ENZYMES (  170 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AvaI        AvaII       AviII       
AvrII       BalI        BamHI       BanI        BbeI        
BbrPI       BbvCI       BcgI        BciVI       BclI        
BcnI        BfrI        BglI        BglII       BlnI        
BpmI        BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiWI       BsmBI       BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstUI       BstXI       
BstZ17I     Bsu36I      CfoI        Cfr10I      Cfr13I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoRV       FauI        FbaI        FseI        
FspI        HaeII       HapII       HgaI        HhaI        
Hin1I       HinP1I      HpaII       KasI        KpnI        
KspI        MamI        MfeI        MluI        MluNI       
MroI        MscI        MslI        MspA1I      MspI        
MunI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NlaIV       
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacII       
SalI        Sau96I      SbfI        ScaI        SexAI       
SfaNI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tth111I     
XbaI        XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AciI          120  GCGG                 CCGC(-3/-1)
AlwI          122  GGATC                GGATC(4/5)
AlwNI         394  CAGGAACTG            CAG[ACGT][ACGT][ACGT]^CTG
AspHI         511  GAGCTC               G[AT]GC[AT]^C
BanII         511  GAGCTC               G[GA]GC[CT]^C
BbvI          684  GCTGC                GCAGC(8/12)
BlpI          513  GCTCAGC              GC^T[ACGT]AGC
BmyI          511  GAGCTC               G[AGT]GC[ACT]^C
Bpu1102I      513  GCTCAGC              GC^T[ACGT]AGC
BsiHKAI       511  GAGCTC               G[AT]GC[AT]^C
BsmAI         827  GAGAC                GTCTC(1/5)
BsmFI         430  GTCCC                GGGAC(10/14)
BsmI          659  GAATGC               GAATGC(1/-1)
Bsp1286I      511  GAGCTC               G[AGT]GC[ACT]^C
BstEII        165  GGTCACC              G^GT[ACGT]ACC
BstF5I        999  GGATG                GGATG(2/0)
BstPI         165  GGTCACC              G^GT[ACGT]ACC
BstYI         122  GGATCT               [GA]^GATC[CT]
CelII         513  GCTCAGC              GC^T[ACGT]AGC
EcoO65I       165  GGTCACC              G^GT[ACGT]ACC
EcoRI         298  GAATTC               G^AATTC
EcoT14I       434  CCAAGG               C^C[AT][AT]GG
EcoT22I       786  ATGCAT               ATGCA^T
Fnu4HI        684  GCTGC                GC^[ACGT]GC
FokI          999  GGATG                GGATG(9/13)
HaeIII        904  GGCC                 GG^CC
HindIII       141  AAGCTT               A^AGCTT
HpaI          481  GTTAAC               GTT^AAC
ItaI          684  GCTGC                GC^[ACGT]GC
MaeII         676  ACGT                 A^CGT
MflI          122  GGATCT               [GA]^GATC[CT]
MwoI           28  GCTGTCAATGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NsiI          786  ATGCAT               ATGCA^T
PflMI         367  CCAGGGATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI          511  GAGCTC               GAGCT^C
SapI          944  GAAGAGC              GCTCTTC(1/4)
SmlI          601  CTTGAG               C^T[CT][GA]AG
StyI          434  CCAAGG               C^C[AT][AT]GG
TaqI          342  TCGA                 T^CGA
TseI          684  GCTGC                G^C[AT]GC
TthHB8I       342  TCGA                 T^CGA
Van91I        367  CCAGGGATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         122  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AfaI           20  GTAC                 GT^AC
              743  GTAC                
Asp700I       397  GAACTGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              634  GAACAACTTC          
BbsI           79  GAAGAC               GAAGAC(2/6)
              216  GAAGAC              
BpuAI          79  GAAGAC               GAAGAC(2/6)
              216  GAAGAC              
BssKI         170  CCAGG                ^CC[ACGT]GG
              367  CCAGG               
BstNI         170  CCAGG                CC^[AT]GG
              367  CCAGG               
EcoRII        170  CCAGG                ^CC[AT]GG
              367  CCAGG               
MaeIII        166  GTCAC                ^GT[ACGT]AC
              936  GTCAC               
MseI          145  TTAA                 T^TAA
              482  TTAA                
MvaI          170  CCAGG                CC^[AT]GG
              367  CCAGG               
NspI          590  ACATGC               [GA]CATG^[CT]
              703  ACATGC              
PleI          185  GAGTC                GAGTC(4/5)
              934  GAGTC               
RsaI           20  GTAC                 GT^AC
              743  GTAC                
ScrFI         170  CCAGG                CC^[ACGT]GG
              367  CCAGG               
SfcI          253  CTACAG               C^T[GA][CT]AG
              650  CTATAG              
Tru9I         145  TTAA                 T^TAA
              482  TTAA                
Tsp45I        166  GTCAC                ^GT[GC]AC
              936  GTCAC               
TspRI         836  CACTG                CA[GC]TG[ACGT][ACGT]^
              864  CAGTG               
XmnI          397  GAACTGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              634  GAACAACTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   26 enzymes) #
#########################################

AcsI        AluI        ApoI        BfaI        BsaJI       
BsiYI       BslI        BsrI        Cac8I       DdeI        
DpnII       EarI        HincII      HindII      HinfI       
HphI        Ksp632I     MaeI        MboI        MboII       
MnlI        NdeII       NlaIII      Sau3AI      TfiI        
Tsp509I     
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