Restriction map for pF1KB8624
##################################
# NO CUT ENZYMES (  117 enzymes) #
##################################

Acc65I      AccIII      AclI        AcsI        AflII       
AflIII      AgeI        AhdI        Alw44I      Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AvrII       BalI        BbrPI       BbvCI       
BciVI       BclI        BfrI        BglII       BlnI        
BsaAI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrGI       BssHII      BssSI       
Bst1107I    BstXI       BstZ17I     ClaI        DraI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoRI       EcoRV       EcoT22I     FbaI        
FseI        HindIII     HpaI        KpnI        KspI        
MfeI        MluI        MluNI       MroI        MscI        
MunI        NdeI        NheI        NotI        NruI        
NsiI        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        SacI        SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TfiI        Tth111I     XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AatII         516  GACGTC               GACGT^C
AccI          184  GTAGAC               GT^[AC][GT]AC
AccII         172  CGCG                 CG^CG
AfaI          333  GTAC                 GT^AC
AocI          125  CCTCAGG              CC^T[ACGT]AGG
AviII         773  TGCGCA               TGC^GCA
BamHI         905  GGATCC               G^GATCC
BcgI          566  GCACCTGCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          134  CTAG                 C^TAG
BglI          313  GCCTCCAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI         411  GATCCCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsrDI         685  CATTGC               GCAATG(2/0)
BstBI          64  TTCGAA               TT^CGAA
BstEII       1032  GGTGACC              G^GT[ACGT]ACC
BstPI        1032  GGTGACC              G^GT[ACGT]ACC
BstUI         172  CGCG                 CG^CG
BstYI         905  GGATCC               [GA]^GATC[CT]
Bsu36I        125  CCTCAGG              CC^T[ACGT]AGG
CpoI          799  CGGACCG              CG^G[AT]CCG
DraIII        889  CACAAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1035  GACCCTGCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        125  CCTCAGG              CC^T[ACGT]AGG
EcoNI         168  CCTGCGCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1032  GGTGACC              G^GT[ACGT]ACC
EcoT14I       504  CCATGG               C^C[AT][AT]GG
FspI          773  TGCGCA               TGC^GCA
HgaI          187  GACGC                GACGC(5/10)
HincII        427  GTCAAC               GT[CT]^[GA]AC
HindII        427  GTCAAC               GT[CT]^[GA]AC
MaeI          134  CTAG                 C^TAG
MaeII         517  ACGT                 A^CGT
MamI          411  GATCCCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          905  GGATCC               [GA]^GATC[CT]
MseI          254  TTAA                 T^TAA
MslI          543  CACCCTGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI          172  CGCG                 CG^CG
NcoI          504  CCATGG               C^CATGG
NspI          854  ACATGC               [GA]CATG^[CT]
NspV           64  TTCGAA               TT^CGAA
PflMI         888  CCACAAAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         950  GGGTCCC              [GA]G^G[AT]CC[CT]
RsaI          333  GTAC                 GT^AC
RsrII         799  CGGACCG              CG^G[AT]CCG
SapI          858  GCTCTTC              GCTCTTC(1/4)
SfuI           64  TTCGAA               TT^CGAA
SgrAI          17  CGCCGGCG             C[GA]^CCGG[CT]G
StyI          504  CCATGG               C^C[AT][AT]GG
Tru9I         254  TTAA                 T^TAA
Tsp509I       444  AATT                 ^AATT
Van91I        888  CCACAAAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          767  CCACACTGCGCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         905  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

ApaI          807  GGGCCC               GGGCC^C
              924  GGGCCC              
BanII         807  GGGCCC               G[GA]GC[CT]^C
              924  GGGCCC              
BbeI           15  GGCGCC               GGCGC^C
               28  GGCGCC              
BbsI          513  GAAGAC               GAAGAC(2/6)
              478  GTCTTC              
BcnI           48  CCCGG                CC^[GC]GG
              534  CCGGG               
BlpI           89  GCTCAGC              GC^T[ACGT]AGC
              264  GCTGAGC             
Bpu1102I       89  GCTCAGC              GC^T[ACGT]AGC
              264  GCTGAGC             
BpuAI         513  GAAGAC               GAAGAC(2/6)
              478  GTCTTC              
BseRI         826  CTCCTC               GAGGAG(10/8)
              220  GAGGAG              
BsmFI         258  GGGAC                GGGAC(10/14)
              952  GTCCC               
BsrFI          18  GCCGGC               [GA]^CCGG[CT]
              269  GCCGGC              
BsrI          581  ACTGG                ACTGG(1/-1)
              918  ACTGG               
BstAPI        196  GCAGCTGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              205  GCAGCTGCTGC         
CelII          89  GCTCAGC              GC^T[ACGT]AGC
              264  GCTGAGC             
Cfr10I         18  GCCGGC               [GA]^CCGG[CT]
              269  GCCGGC              
DsaI          504  CCATGG               C^C[GA][CT]GG
              803  CCGTGG              
FauI          936  CCCGC                CCCGC(4/6)
               22  GCGGG               
HinfI         403  GAGTC                G^A[ACGT]TC
              590  GAGTC               
KasI           15  GGCGCC               G^GCGCC
               28  GGCGCC              
MaeIII       1033  GTGAC                ^GT[ACGT]AC
             1044  GTCAC               
NaeI           18  GCCGGC               GCC^GGC
              269  GCCGGC              
NarI           15  GGCGCC               GG^CGCC
               28  GGCGCC              
NciI           48  CCCGG                CC^[GC]GG
              534  CCGGG               
NgoMIV         18  GCCGGC               G^CCGGC
              269  GCCGGC              
PleI          403  GAGTC                GAGTC(4/5)
              590  GAGTC               
PstI          646  CTGCAG               CTGCA^G
             1039  CTGCAG              
SfoI           15  GGCGCC               GGC^GCC
               28  GGCGCC              
Tsp45I       1033  GTGAC                ^GT[GC]AC
             1044  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   61 enzymes) #
#########################################

AciI        AcyI        AluI        AlwI        AlwNI       
AvaII       BanI        BbvI        BmyI        BpmI        
BsaHI       BsaJI       BsiYI       BslI        BsmAI       
Bsp1286I    BspMI       BssKI       BstF5I      BstNI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       EarI        EcoO109I    EcoRII      Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
Hin1I       HinP1I      HpaII       HphI        ItaI        
Ksp632I     MboI        MboII       MnlI        MspA1I      
MspI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       PvuII       Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        TaqI        TseI        TspRI       
TthHB8I     
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