Restriction map for pF1KB8624
 (Linear) (Six-base) MAPSORT of: KIBB8624.seq  Check: 7083  from: 1  to: 1050



KIBB8624.orf

 With 182 enzymes: SgfI * 

                        July 2, 2008 13:23  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     264     439     577     639    1050 
   Size:        264     175     138      62     411

  Fragments arranged by size: 

                 411     264     175     138      62
 
AatII G_ACGT'C

Cuts at:      0     520    1050 
   Size:        520     530
 
AccI GT'mk_AC

Cuts at:      0     185    1050 
   Size:        185     865
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     236     309    1050 
   Size:        236      73     741

  Fragments arranged by size: 

                 741     236      73
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     217     249    1050 
   Size:        217      32     801

  Fragments arranged by size: 

                 801     217      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     168     211     822    1050 
   Size:        168      43     611     228

  Fragments arranged by size: 

                 611     228     168      43
 
ApaI G_GGCC'C

Cuts at:      0     811     928    1050 
   Size:        811     117     122

  Fragments arranged by size: 

                 811     122     117
 
BamHI G'GATC_C

Cuts at:      0     905    1050 
   Size:        905     145
 
BanI G'GyrC_C

Cuts at:      0      15      28     541     565     835    1050 
   Size:         15      13     513      24     270     215

  Fragments arranged by size: 

                 513     270     215      24      15      13
 
BanII G_rGCy'C

Cuts at:      0     811     928    1050 
   Size:        811     117     122

  Fragments arranged by size: 

                 811     122     117
 
BbeI G_GCGC'C

Cuts at:      0      19      32    1050 
   Size:         19      13    1018

  Fragments arranged by size: 

                1018      19      13
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     471     520    1050 
   Size:        471      49     530

  Fragments arranged by size: 

                 530     471      49
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     555     589    1050 
   Size:        555      34     461

  Fragments arranged by size: 

                 555     461      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     319    1050 
   Size:        319     731
 
BlpI GC'TnA_GC

Cuts at:      0      90     265    1050 
   Size:         90     175     785

  Fragments arranged by size: 

                 785     175      90
 
Bme1580I G_kGCm'C

Cuts at:      0     544     556     811     928    1050 
   Size:        544      12     255     117     122

  Fragments arranged by size: 

                 544     255     122     117      12
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     300     421     940    1050 
   Size:        300     121     519     110

  Fragments arranged by size: 

                 519     300     121     110
 
Bpu10I CC'TnA_GC

Cuts at:      0     380    1050 
   Size:        380     670
 
BsaBI GATnn'nnATC

Cuts at:      0     415    1050 
   Size:        415     635
 
BsaHI Gr'CG_yC

Cuts at:      0      16      29     517    1050 
   Size:         16      13     488     533

  Fragments arranged by size: 

                 533     488      16      13
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     235     817    1050 
   Size:        235     582     233

  Fragments arranged by size: 

                 582     235     233
 
BseYI C'CCAG_C

Cuts at:      0     298     585     663    1050 
   Size:        298     287      78     387

  Fragments arranged by size: 

                 387     298     287      78
 
Bsp1286I G_dGCh'C

Cuts at:      0     544     556     811     928    1050 
   Size:        544      12     255     117     122

  Fragments arranged by size: 

                 544     255     122     117      12
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     264     439     577     639    1050 
   Size:        264     175     138      62     411

  Fragments arranged by size: 

                 411     264     175     138      62
 
BsrDI GCAATG_nn'

Cuts at:      0     684    1050 
   Size:        684     366
 
BsrFI r'CCGG_y

Cuts at:      0      18     269    1050 
   Size:         18     251     781

  Fragments arranged by size: 

                 781     251      18
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     202     211    1050 
   Size:        202       9     839

  Fragments arranged by size: 

                 839     202       9
 
BstBI TT'CG_AA

Cuts at:      0      65    1050 
   Size:         65     985
 
BstEII G'GTnAC_C

Cuts at:      0    1032    1050 
   Size:       1032      18
 
BstYI r'GATC_y

Cuts at:      0     905    1050 
   Size:        905     145
 
Bsu36I CC'TnA_GG

Cuts at:      0     126    1050 
   Size:        126     924
 
BtgI C'CryG_G

Cuts at:      0     504     803    1050 
   Size:        504     299     247

  Fragments arranged by size: 

                 504     299     247
 
BtsI GCAGTG_nn'

Cuts at:      0     658     769     791    1050 
   Size:        658     111      22     259

  Fragments arranged by size: 

                 658     259     111      22
 
DraIII CAC_nnn'GTG

Cuts at:      0     894    1050 
   Size:        894     156
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1041    1050 
   Size:       1041       9
 
EarI CTCTTCn'nnn_

Cuts at:      0     212     736     745     865    1050 
   Size:        212     524       9     120     185

  Fragments arranged by size: 

                 524     212     185     120       9
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      98    1050 
   Size:         98     952
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     236     300     309     421     940    1050 
   Size:        236      64       9     112     519     110

  Fragments arranged by size: 

                 519     236     112     110      64       9
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     172    1050 
   Size:        172     878
 
EcoO109I rG'GnC_Cy

Cuts at:      0     320     808     924     925     951    1050 
   Size:        320     488     116       1      26      99

  Fragments arranged by size: 

                 488     320     116      99      26       1
 
FspI TGC'GCA

Cuts at:      0     775    1050 
   Size:        775     275
 
HaeII r_GCGC'y

Cuts at:      0      19      32     168    1050 
   Size:         19      13     136     882

  Fragments arranged by size: 

                 882     136      19      13
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     109     141    1050 
   Size:        109      32     909

  Fragments arranged by size: 

                 909     109      32
 
HincII GTy'rAC

Cuts at:      0     429    1050 
   Size:        429     621
 
KasI G'GCGC_C

Cuts at:      0      15      28    1050 
   Size:         15      13    1022

  Fragments arranged by size: 

                1022      15      13
 
MslI CAynn'nnrTG

Cuts at:      0     547    1050 
   Size:        547     503
 
MspA1I CmG'CkG

Cuts at:      0      13      94     199     208     264     819    1050 
   Size:         13      81     105       9      56     555     231

  Fragments arranged by size: 

                 555     231     105      81      56      13       9
 
NaeI GCC'GGC

Cuts at:      0      20     271    1050 
   Size:         20     251     779

  Fragments arranged by size: 

                 779     251      20
 
NarI GG'CG_CC

Cuts at:      0      16      29    1050 
   Size:         16      13    1021

  Fragments arranged by size: 

                1021      16      13
 
NcoI C'CATG_G

Cuts at:      0     504    1050 
   Size:        504     546
 
NgoMIV G'CCGG_C

Cuts at:      0      18     269    1050 
   Size:         18     251     781

  Fragments arranged by size: 

                 781     251      18
 
NspI r_CATG'y

Cuts at:      0     858    1050 
   Size:        858     192
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     894    1050 
   Size:        894     156
 
PfoI T'CCnGG_A

Cuts at:      0     398     533     908    1050 
   Size:        398     135     375     142

  Fragments arranged by size: 

                 398     375     142     135
 
PpuMI rG'GwC_Cy

Cuts at:      0     951    1050 
   Size:        951      99
 
PspOMI G'GGCC_C

Cuts at:      0     807     924    1050 
   Size:        807     117     126

  Fragments arranged by size: 

                 807     126     117
 
PstI C_TGCA'G

Cuts at:      0     650    1043    1050 
   Size:        650     393       7

  Fragments arranged by size: 

                 650     393       7
 
PvuII CAG'CTG

Cuts at:      0      94     199     208     264    1050 
   Size:         94     105       9      56     786

  Fragments arranged by size: 

                 786     105      94      56       9
 
RsrII CG'GwC_CG

Cuts at:      0     800    1050 
   Size:        800     250
 
SanDI GG'GwC_CC

Cuts at:      0     951    1050 
   Size:        951      99
 
SapI GCTCTTCn'nnn_

Cuts at:      0     865    1050 
   Size:        865     185
 
SfcI C'TryA_G

Cuts at:      0       8     646    1039    1050 
   Size:          8     638     393      11

  Fragments arranged by size: 

                 638     393      11       8
 
SfoI GGC'GCC

Cuts at:      0      17      30    1050 
   Size:         17      13    1020

  Fragments arranged by size: 

                1020      17      13
 
SgrAI Cr'CCGG_yG

Cuts at:      0      18    1050 
   Size:         18    1032
 
StyI C'CwwG_G

Cuts at:      0     504    1050 
   Size:        504     546
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     139     745    1050 
   Size:        139     606     305

  Fragments arranged by size: 

                 606     305     139
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     774    1050 
   Size:        774     276
 
ZraI GAC'GTC

Cuts at:      0     518    1050 
   Size:        518     532
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AloI    AlwNI     ApaI    BamHI
     BanI    BanII     BbeI     BbsI     BcgI     BglI     BlpI Bme1580I
     BpmI   Bpu10I    BsaBI    BsaHI    BseRI    BseYI Bsp1286I    BspMI
    BsrDI    BsrFI   BstAPI    BstBI   BstEII    BstYI   Bsu36I     BtgI
     BtsI   DraIII     DrdI     EarI     EciI  Eco57MI    EcoNI EcoO109I
     FspI    HaeII    Hin4I   HincII     KasI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NspI    PflMI     PfoI    PpuMI   PspOMI
     PstI    PvuII    RsrII    SanDI     SapI     SfcI     SfoI    SgrAI
     StyI    TaqII     XcmI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI     AleI
     AlfI    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BbvCI    BciVI     BclI    BfrBI    BglII    BmgBI     BmrI
     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaWI    BsaXI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI
    BsrGI   BssHII    BssSI    BstXI  BstZ17I    BtgZI     ClaI    CspCI
     DraI     EaeI     EagI  EcoICRI    EcoRI    EcoRV     FalI     FseI
    FspAI  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI
     SacI    SacII     SalI     SbfI     ScaI    SexAI     SfiI     SgfI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
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