Restriction map for pF1KB8619
##################################
# NO CUT ENZYMES (  142 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BamHI       
BbeI        BbvCI       BcgI        BciVI       BclI        
BfaI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BsaBI       BsaHI       BsaI        BsgI        
BsiHKAI     BsiWI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraI        
DrdI        Eam1105I    EarI        Eco47III    Eco81I      
EcoNI       EcoRV       EcoT22I     FauI        FbaI        
FseI        FspI        Hin1I       HincII      HindII      
HindIII     HpaI        KasI        KpnI        Ksp632I     
KspI        MaeI        MamI        MfeI        MluI        
MseI        MunI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmeI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
SspBI       SspI        StuI        SwaI        Tru9I       
Tth111I     Van91I      XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AccIII        858  TCCGGA               T^CCGGA
AcsI           31  GAATTC               [GA]^AATT[CT]
AfaI          837  GTAC                 GT^AC
ApaI          583  GGGCCC               GGGCC^C
ApoI           31  GAATTC               [GA]^AATT[CT]
BbrPI         115  CACGTG               CAC^GTG
BbsI          634  GTCTTC               GAAGAC(2/6)
BpmI          598  CTGGAG               CTGGAG(16/14)
BpuAI         634  GTCTTC               GAAGAC(2/6)
BsaAI         115  CACGTG               [CT]AC^GT[GA]
BseAI         858  TCCGGA               T^CCGGA
BsmAI         601  GAGAC                GTCTC(1/5)
BspEI         858  TCCGGA               T^CCGGA
BstEII        483  GGTGACC              G^GT[ACGT]ACC
BstPI         483  GGTGACC              G^GT[ACGT]ACC
BstXI         764  CCAGGCTGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI          135  CTCAG                C^T[ACGT]AG
DraIII        478  CACAAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I       483  GGTGACC              G^GT[ACGT]ACC
EcoRI          31  GAATTC               G^AATTC
HaeII         696  GGCGCT               [GA]GCGC^[CT]
HgaI          423  GACGC                GACGC(5/10)
MroI          858  TCCGGA               T^CCGGA
MspA1I         97  CAGCTG               C[AC]G^C[GT]G
PmaCI         115  CACGTG               CAC^GTG
PmlI          115  CACGTG               CAC^GTG
PvuII          97  CAGCTG               CAG^CTG
RsaI          837  GTAC                 GT^AC
SbfI          195  CCTGCAGG             CCTGCA^GG
Sse8387I      195  CCTGCAGG             CCTGCA^GG
TaqI           35  TCGA                 T^CGA
Tsp509I        32  AATT                 ^AATT
TthHB8I        35  TCGA                 T^CGA
XcmI           44  CCACTAATCTTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AccII         189  CGCG                 CG^CG
              909  CGCG                
BanI          335  GGCACC               G^G[CT][GA]CC
              525  GGTGCC              
BanII         132  GGGCTC               G[GA]GC[CT]^C
              583  GGGCCC              
BglI          385  GCCACGTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              811  GCCCTCCTGGC         
BsaWI         287  ACCGGA               [AT]^CCGG[AT]
              858  TCCGGA              
BsiEI           8  CGGCCG               CG[GA][CT]^CG
              352  CGGCCG              
BstAPI        336  GCACCATCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              503  GCATCTACTGC         
BstUI         189  CGCG                 CG^CG
              909  CGCG                
BstYI         357  GGATCT               [GA]^GATC[CT]
              377  GGATCT              
EagI            8  CGGCCG               C^GGCCG
              352  CGGCCG              
EclXI           8  CGGCCG               C^GGCCG
              352  CGGCCG              
Eco52I          8  CGGCCG               C^GGCCG
              352  CGGCCG              
HinfI         227  GATTC                G^A[ACGT]TC
              348  GAATC               
HphI          483  GGTGA                GGTGA(8/7)
              734  TCACC               
MflI          357  GGATCT               [GA]^GATC[CT]
              377  GGATCT              
MslI          639  CATCCCCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              663  CATCCCTGTG          
MvnI          189  CGCG                 CG^CG
              909  CGCG                
NcoI          644  CCATGG               C^CATGG
              821  CCATGG              
PpuMI         278  AGGTCCT              [GA]G^G[AT]CC[CT]
              446  GGGACCC             
PstI          196  CTGCAG               CTGCA^G
              679  CTGCAG              
TfiI          227  GATTC                G^A[AT]TC
              348  GAATC               
XhoII         357  GGATCT               [GA]^GATC[CT]
              377  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   60 enzymes) #
#########################################

AciI        AluI        AlwI        AlwNI       AvaII       
BalI        BbvI        BcnI        BmyI        BsaJI       
BseRI       BsiYI       BslI        BsmFI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       Cac8I       
CfoI        Cfr13I      DpnII       DraII       DsaI        
EaeI        EcoO109I    EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HpaII       ItaI        MaeII       MaeIII      MboI        
MboII       MluNI       MnlI        MscI        MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StyI        TseI        Tsp45I      TspRI       
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