Restriction map for pF1KB8619
 (Linear) (Six-base) MAPSORT of: KIBB8619.seq  Check: 7823  from: 1  to: 948



KIBB8619.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:06  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     222     948 
   Size:        222     726
 
AlwNI CAG_nnn'CTG

Cuts at:      0     282     852     936     948 
   Size:        282     570      84      12

  Fragments arranged by size: 

                 570     282      84      12
 
ApaI G_GGCC'C

Cuts at:      0     587     948 
   Size:        587     361
 
ApoI r'AATT_y

Cuts at:      0      31     948 
   Size:         31     917
 
BanI G'GyrC_C

Cuts at:      0     335     525     948 
   Size:        335     190     423

  Fragments arranged by size: 

                 423     335     190
 
BanII G_rGCy'C

Cuts at:      0     136     587     948 
   Size:        136     451     361

  Fragments arranged by size: 

                 451     361     136
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     627     948 
   Size:        627     321
 
BglI GCCn_nnn'nGGC

Cuts at:      0     391     817     948 
   Size:        391     426     131

  Fragments arranged by size: 

                 426     391     131
 
Bme1580I G_kGCm'C

Cuts at:      0     116     530     587     948 
   Size:        116     414      57     361

  Fragments arranged by size: 

                 414     361     116      57
 
BmgBI CAC'GTC

Cuts at:      0     264     948 
   Size:        264     684
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     465     948 
   Size:        465     483
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     619     948 
   Size:        619     329
 
Bpu10I CC'TnA_GC

Cuts at:      0     135     948 
   Size:        135     813
 
BsaAI yAC'GTr

Cuts at:      0     117     948 
   Size:        117     831
 
BsaWI w'CCGG_w

Cuts at:      0     287     858     948 
   Size:        287     571      90

  Fragments arranged by size: 

                 571     287      90
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     592     607     622     637     948 
   Size:        592      15      15      15     311

  Fragments arranged by size: 

                 592     311      15      15      15
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      52     184     187     456     863     866     948 
   Size:         52     132       3     269     407       3      82

  Fragments arranged by size: 

                 407     269     132      82      52       3       3
 
BsiEI CG_ry'CG

Cuts at:      0      11     355     948 
   Size:         11     344     593

  Fragments arranged by size: 

                 593     344      11
 
Bsp1286I G_dGCh'C

Cuts at:      0     116     136     530     587     948 
   Size:        116      20     394      57     361

  Fragments arranged by size: 

                 394     361     116      57      20
 
BspEI T'CCGG_A

Cuts at:      0     858     948 
   Size:        858      90
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     342     509     948 
   Size:        342     167     439

  Fragments arranged by size: 

                 439     342     167
 
BstEII G'GTnAC_C

Cuts at:      0     483     948 
   Size:        483     465
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     771     948 
   Size:        771     177
 
BstYI r'GATC_y

Cuts at:      0     357     377     948 
   Size:        357      20     571

  Fragments arranged by size: 

                 571     357      20
 
BtgI C'CryG_G

Cuts at:      0     128     488     644     746     791     821     948 
   Size:        128     360     156     102      45      30     127

  Fragments arranged by size: 

                 360     156     128     127     102      45      30
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     891     948 
   Size:        891      57
 
BtsI GCAGTG_nn'

Cuts at:      0     301     688     948 
   Size:        301     387     260

  Fragments arranged by size: 

                 387     301     260
 
DraIII CAC_nnn'GTG

Cuts at:      0     483     948 
   Size:        483     465
 
EaeI y'GGCC_r

Cuts at:      0       8     125     318     352     404     773     818     948 
   Size:          8     117     193      34      52     369      45     130

  Fragments arranged by size: 

                 369     193     130     117      52      45      34       8
            
 
EagI C'GGCC_G

Cuts at:      0       8     352     948 
   Size:          8     344     596

  Fragments arranged by size: 

                 596     344       8
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     222     619     948 
   Size:        222     397     329

  Fragments arranged by size: 

                 397     329     222
 
EcoO109I rG'GnC_Cy

Cuts at:      0     279     447     583     584     948 
   Size:        279     168     136       1     364

  Fragments arranged by size: 

                 364     279     168     136       1
 
EcoRI G'AATT_C

Cuts at:      0      31     948 
   Size:         31     917
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     855     948 
   Size:        855      93
 
HaeII r_GCGC'y

Cuts at:      0     700     948 
   Size:        700     248
 
MscI TGG'CCA

Cuts at:      0     320     775     820     948 
   Size:        320     455      45     128

  Fragments arranged by size: 

                 455     320     128      45
 
MslI CAynn'nnrTG

Cuts at:      0     643     667     948 
   Size:        643      24     281

  Fragments arranged by size: 

                 643     281      24
 
MspA1I CmG'CkG

Cuts at:      0      99     948 
   Size:         99     849
 
NcoI C'CATG_G

Cuts at:      0     644     821     948 
   Size:        644     177     127

  Fragments arranged by size: 

                 644     177     127
 
PasI CC'CwG_GG

Cuts at:      0     705     948 
   Size:        705     243
 
PfoI T'CCnGG_A

Cuts at:      0     281     569     596     948 
   Size:        281     288      27     352

  Fragments arranged by size: 

                 352     288     281      27
 
PmlI CAC'GTG

Cuts at:      0     117     948 
   Size:        117     831
 
PpuMI rG'GwC_Cy

Cuts at:      0     279     447     948 
   Size:        279     168     501

  Fragments arranged by size: 

                 501     279     168
 
PspOMI G'GGCC_C

Cuts at:      0     583     948 
   Size:        583     365
 
PstI C_TGCA'G

Cuts at:      0     200     683     948 
   Size:        200     483     265

  Fragments arranged by size: 

                 483     265     200
 
PvuII CAG'CTG

Cuts at:      0      99     948 
   Size:         99     849
 
SanDI GG'GwC_CC

Cuts at:      0     447     948 
   Size:        447     501
 
SbfI CC_TGCA'GG

Cuts at:      0     200     948 
   Size:        200     748
 
SfcI C'TryA_G

Cuts at:      0     196     462     679     948 
   Size:        196     266     217     269

  Fragments arranged by size: 

                 269     266     217     196
 
StyI C'CwwG_G

Cuts at:      0     644     673     821     948 
   Size:        644      29     148     127

  Fragments arranged by size: 

                 644     148     127      29
 
TatI w'GTAC_w

Cuts at:      0     836     948 
   Size:        836     112
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     592     624     948 
   Size:        592      32     324

  Fragments arranged by size: 

                 592     324      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      51     948 
   Size:         51     897
 

 Enzymes that do cut:

     AcuI    AlwNI     ApaI     ApoI     BanI    BanII     BbsI     BglI
 Bme1580I    BmgBI     BmrI     BpmI   Bpu10I    BsaAI    BsaWI    BsaXI
    BseRI    BsiEI Bsp1286I    BspEI   BstAPI   BstEII    BstXI    BstYI
     BtgI    BtgZI     BtsI   DraIII     EaeI     EagI  Eco57MI EcoO109I
    EcoRI     FalI    HaeII     MscI     MslI   MspA1I     NcoI     PasI
     PfoI     PmlI    PpuMI   PspOMI     PstI    PvuII    SanDI     SbfI
     SfcI     StyI     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI    BglII     BlpI     BmtI     BplI    BpuEI
     BsaI    BsaBI    BsaHI    BseYI     BsgI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI    BstBI  BstZ17I   Bsu36I     ClaI    CspCI     DraI     DrdI
     EarI     EciI  EcoICRI    EcoNI    EcoRV     FseI     FspI    FspAI
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PciI    PflMI     PmeI     PpiI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
     SapI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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