Restriction map for pF1KB8617
##################################
# NO CUT ENZYMES (  147 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AvaII       AviII       
AvrII       BbeI        BbrPI       BbvCI       BciVI       
BcnI        BfrI        BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsiEI       BsiWI       BsmBI       
Bsp1407I    BspDI       BspEI       BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstZ17I     CelII       Cfr10I      ClaI        
CpoI        DraII       DraIII      DrdI        DsaI        
EagI        Eam1105I    EclXI       Eco52I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FauI        FseI        FspI        HapII       
HgaI        Hin1I       HincII      HindII      HindIII     
HpaI        HpaII       KasI        KpnI        KspI        
MamI        MfeI        MluI        MroI        MspI        
MunI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        RsrII       SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StyI        
SwaI        Tth111I     Van91I      XbaI        XhoI        
XmaCI       XmaI        


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# ONE CUT ENZYMES (   40 enzymes) #
###################################

AccI          499  GTCTAC               GT^[AC][GT]AC
AciI         1438  CCGC                 CCGC(-3/-1)
AfaI         1249  GTAC                 GT^AC
AflIII        963  ACATGT               A^C[GA][CT]GT
AocI          631  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       293  AGCGCT               AGC^GCT
AvaI          229  CCCGAG               C^[CT]CG[GA]G
BamHI        1702  GGATCC               G^GATCC
BanI          672  GGTGCC               G^G[CT][GA]CC
BcgI          231  CGAGCAGGGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         1532  TGATCA               T^GATCA
BglI          191  GCCCAGGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI          737  GTGCAG               GTGCAG(16/14)
BsoBI         229  CCCGAG               C^[CT]CG[GA]G
BspHI        1743  TCATGA               T^CATGA
BspLU11I      963  ACATGT               A^CATGT
BstXI        1590  CCATCAGATTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        631  CCTGAGG              CC^T[ACGT]AGG
Cfr13I        599  GGCCC                G^G[ACGT]CC
DraI         1308  TTTAAA               TTT^AAA
Eco47III      293  AGCGCT               AGC^GCT
Eco81I        631  CCTGAGG              CC^T[ACGT]AGG
FbaI         1532  TGATCA               T^GATCA
HaeII         293  AGCGCT               [GA]GCGC^[CT]
HphI          593  TCACC                GGTGA(8/7)
MaeII        1251  ACGT                 A^CGT
MslI          684  CATTCAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NheI         1179  GCTAGC               G^CTAGC
NspI          963  ACATGT               [GA]CATG^[CT]
RcaI         1743  TCATGA               T^CATGA
RsaI         1249  GTAC                 GT^AC
SacI         1017  GAGCTC               GAGCT^C
SapI          262  GAAGAGC              GCTCTTC(1/4)
Sau96I        599  GGCCC                G^G[ACGT]CC
SfaNI         127  GATGC                GCATC(5/9)
StuI         1521  AGGCCT               AGG^CCT
TaqI          800  TCGA                 T^CGA
Tsp45I        592  GTCAC                ^GT[GC]AC
TthHB8I       800  TCGA                 T^CGA
XcmI         1070  CCATGTTAAAGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AlwI         1703  GATCC                GGATC(4/5)
             1702  GGATC               
BalI          198  TGGCCA               TGG^CCA
              731  TGGCCA              
BanII         265  GAGCCC               G[GA]GC[CT]^C
             1017  GAGCTC              
BglII          13  AGATCT               A^GATCT
             1371  AGATCT              
BpmI         1334  CTCCAG               CTGGAG(16/14)
             1220  CTGGAG              
BsmI           33  GAATGC               GAATGC(1/-1)
              486  GAATGC              
BstF5I        253  CATCC                GGATG(2/0)
             1578  GGATG               
CfoI          294  GCGC                 GCG^C
              820  GCGC                
EaeI          198  TGGCCA               [CT]^GGCC[GA]
              731  TGGCCA              
FokI          253  CATCC                GGATG(9/13)
             1578  GGATG               
HhaI          294  GCGC                 GCG^C
              820  GCGC                
HinP1I        294  GCGC                 G^CGC
              820  GCGC                
MaeIII        592  GTCAC                ^GT[ACGT]AC
             1798  GTTAC               
MluNI         198  TGGCCA               TGG^CCA
              731  TGGCCA              
MscI          198  TGGCCA               TGG^CCA
              731  TGGCCA              
MspA1I        562  CAGCTG               C[AC]G^C[GT]G
              761  CAGCTG              
PvuII         562  CAGCTG               CAG^CTG
              761  CAGCTG              


#########################################
# 3 OR MORE CUT ENZYMES (   54 enzymes) #
#########################################

AcsI        AluI        AlwNI       ApoI        Asp700I     
AspHI       BbsI        BbvI        BfaI        BmyI        
BpuAI       BsaJI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrI        BssKI       
BstNI       BstYI       Cac8I       DdeI        DpnII       
EarI        EcoRII      Fnu4HI      HaeIII      HinfI       
ItaI        Ksp632I     MaeI        MboI        MboII       
MflI        MnlI        MseI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       PleI        Sau3AI      
ScrFI       SfcI        TfiI        Tru9I       TseI        
Tsp509I     TspRI       XhoII       XmnI        
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