Restriction map for pF1KB8617
 (Linear) (Six-base) MAPSORT of: KIBB8617.seq  Check: 3980  from: 1  to: 1857



KIBB8617.orf

 With 182 enzymes: SgfI * 

                        September 18, 2009 17:00  ..

AccI GT'mk_AC

Cuts at:      0     500    1857 
   Size:        500    1357
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79     281     514    1857 
   Size:         79     202     233    1343

  Fragments arranged by size: 

                1343     233     202      79
 
AfeI AGC'GCT

Cuts at:      0     295    1857 
   Size:        295    1562
 
AflIII A'CryG_T

Cuts at:      0     963    1857 
   Size:        963     894
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     560     592    1857 
   Size:        560      32    1265

  Fragments arranged by size: 

                1265     560      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     564     745    1194    1857 
   Size:        564     181     449     663

  Fragments arranged by size: 

                 663     564     449     181
 
ApoI r'AATT_y

Cuts at:      0     569    1304    1728    1857 
   Size:        569     735     424     129

  Fragments arranged by size: 

                 735     569     424     129
 
AvaI C'yCGr_G

Cuts at:      0     229    1857 
   Size:        229    1628
 
BamHI G'GATC_C

Cuts at:      0    1702    1857 
   Size:       1702     155
 
BanI G'GyrC_C

Cuts at:      0     672    1857 
   Size:        672    1185
 
BanII G_rGCy'C

Cuts at:      0     269    1021    1857 
   Size:        269     752     836

  Fragments arranged by size: 

                 836     752     269
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      95     542    1280    1857 
   Size:         95     447     738     577

  Fragments arranged by size: 

                 738     577     447      95
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     220     254    1857 
   Size:        220      34    1603

  Fragments arranged by size: 

                1603     220      34
 
BclI T'GATC_A

Cuts at:      0    1532    1857 
   Size:       1532     325
 
BglI GCCn_nnn'nGGC

Cuts at:      0     197    1857 
   Size:        197    1660
 
BglII A'GATC_T

Cuts at:      0      13    1371    1857 
   Size:         13    1358     486

  Fragments arranged by size: 

                1358     486      13
 
BmtI G_CTAG'C

Cuts at:      0    1183    1857 
   Size:       1183     674
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     827    1857 
   Size:        827    1030
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1241    1319    1857 
   Size:       1241      78     538

  Fragments arranged by size: 

                1241     538      78
 
Bpu10I CC'TnA_GC

Cuts at:      0     750    1857 
   Size:        750    1107
 
BseYI C'CCAG_C

Cuts at:      0     187     296     564    1278    1857 
   Size:        187     109     268     714     579

  Fragments arranged by size: 

                 714     579     268     187     109
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     758    1857 
   Size:        758    1099
 
BsiHKAI G_wGCw'C

Cuts at:      0     512    1021    1504    1857 
   Size:        512     509     483     353

  Fragments arranged by size: 

                 512     509     483     353
 
BsmI GAATG_Cn'

Cuts at:      0      39     492    1857 
   Size:         39     453    1365

  Fragments arranged by size: 

                1365     453      39
 
Bsp1286I G_dGCh'C

Cuts at:      0     269     512    1021    1504    1857 
   Size:        269     243     509     483     353

  Fragments arranged by size: 

                 509     483     353     269     243
 
BspHI T'CATG_A

Cuts at:      0    1743    1857 
   Size:       1743     114
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1597    1857 
   Size:       1597     260
 
BstYI r'GATC_y

Cuts at:      0      13    1371    1702    1857 
   Size:         13    1358     331     155

  Fragments arranged by size: 

                1358     331     155      13
 
Bsu36I CC'TnA_GG

Cuts at:      0     632    1857 
   Size:        632    1225
 
DraI TTT'AAA

Cuts at:      0    1310    1857 
   Size:       1310     547
 
EaeI y'GGCC_r

Cuts at:      0     198     731    1857 
   Size:        198     533    1126

  Fragments arranged by size: 

                1126     533     198
 
EarI CTCTTCn'nnn_

Cuts at:      0     257     422     714    1596    1672    1747    1857 
   Size:        257     165     292     882      76      75     110

  Fragments arranged by size: 

                 882     292     257     165     110      76      75
 
EcoICRI GAG'CTC

Cuts at:      0    1019    1857 
   Size:       1019     838
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79     281     514    1241    1319    1857 
   Size:         79     202     233     727      78     538

  Fragments arranged by size: 

                 727     538     233     202      79      78
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1341    1857 
   Size:       1341     516
 
HaeII r_GCGC'y

Cuts at:      0     297    1857 
   Size:        297    1560
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     335     367     460     492     534     566     733     765 
   Size:        335      32      93      32      42      32     167      32

Cuts at:    765    1857 
   Size:       1092

  Fragments arranged by size: 

                1092     335     167      93      42      32      32      32
                  32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      80    1186    1857 
   Size:         80    1106     671

  Fragments arranged by size: 

                1106     671      80
 
MscI TGG'CCA

Cuts at:      0     200     733    1857 
   Size:        200     533    1124

  Fragments arranged by size: 

                1124     533     200
 
MslI CAynn'nnrTG

Cuts at:      0     688    1857 
   Size:        688    1169
 
MspA1I CmG'CkG

Cuts at:      0     564     763    1857 
   Size:        564     199    1094

  Fragments arranged by size: 

                1094     564     199
 
NheI G'CTAG_C

Cuts at:      0    1179    1857 
   Size:       1179     678
 
NspI r_CATG'y

Cuts at:      0     967    1857 
   Size:        967     890
 
PasI CC'CwG_GG

Cuts at:      0     988    1857 
   Size:        988     869
 
PciI A'CATG_T

Cuts at:      0     963    1857 
   Size:        963     894
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1620    1652    1857 
   Size:       1620      32     205

  Fragments arranged by size: 

                1620     205      32
 
PvuII CAG'CTG

Cuts at:      0     564     763    1857 
   Size:        564     199    1094

  Fragments arranged by size: 

                1094     564     199
 
SacI G_AGCT'C

Cuts at:      0    1021    1857 
   Size:       1021     836
 
SapI GCTCTTCn'nnn_

Cuts at:      0     257    1857 
   Size:        257    1600
 
SfcI C'TryA_G

Cuts at:      0     131     137    1022    1857 
   Size:        131       6     885     835

  Fragments arranged by size: 

                 885     835     131       6
 
StuI AGG'CCT

Cuts at:      0    1523    1857 
   Size:       1523     334
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1077    1857 
   Size:       1077     780
 
XmnI GAAnn'nnTTC

Cuts at:      0      92    1118    1388    1857 
   Size:         92    1026     270     469

  Fragments arranged by size: 

                1026     469     270      92
 

 Enzymes that do cut:

     AccI     AcuI     AfeI   AflIII     AloI    AlwNI     ApoI     AvaI
    BamHI     BanI    BanII     BbsI     BcgI     BclI     BglI    BglII
     BmtI     BplI     BpmI   Bpu10I    BseYI     BsgI  BsiHKAI     BsmI
 Bsp1286I    BspHI    BstXI    BstYI   Bsu36I     DraI     EaeI     EarI
  EcoICRI  Eco57MI     FalI    HaeII    Hin4I     MmeI     MscI     MslI
   MspA1I     NheI     NspI     PasI     PciI     PsrI    PvuII     SacI
     SapI     SfcI     StuI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII     AgeI     AhdI     AleI
     AlfI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
     BbeI    BbvCI    BciVI    BfrBI     BlpI Bme1580I    BmgBI     BmrI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BsiEI    BsiWI    BsmBI    BspEI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I     BtgI
    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI     EagI     EciI
    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NotI     NruI     NsiI     PacI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PstI     PvuI    RsrII    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     ZraI
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