Restriction map for pF1KB8589
##################################
# NO CUT ENZYMES (  149 enzymes) #
##################################

AccII       AccIII      AclI        AflII       AgeI        
AhdI        AlwI        AocI        Aor51HI     ApaI        
AscI        AseI        AsnI        Asp700I     AspEI       
AspI        AvaII       AviII       AvrII       BalI        
BamHI       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BcnI        BfrI        BglI        BlnI        
BlpI        BpmI        Bpu1102I    BsaAI       BsaBI       
BsaI        BsaWI       BseAI       BseRI       BsiWI       
BsmBI       BsmI        Bsp1407I    BspEI       BspMI       
BsrDI       BsrFI       BsrGI       BssHII      BssKI       
BssSI       Bst1107I    BstBI       BstEII      BstNI       
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
CelII       Cfr10I      CpoI        DraI        DrdI        
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRII      EcoRV       EcoT14I     
FauI        FseI        FspI        HaeII       HapII       
HgaI        HindIII     HpaII       KasI        Ksp632I     
KspI        MamI        MfeI        MluI        MluNI       
MroI        MscI        MspI        MunI        MvaI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    RsrII       SacII       
SapI        SbfI        ScaI        ScrFI       SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SphI        Sse8387I    SspBI       SspI        
StyI        SwaI        Tth111I     Van91I      XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   59 enzymes) #
###################################

AatII         479  GACGTC               GACGT^C
Acc65I        828  GGTACC               G^GTACC
AccI          218  GTCGAC               GT^[AC][GT]AC
AcsI          227  AAATTC               [GA]^AATT[CT]
AcyI          479  GACGTC               G[GA]^CG[CT]C
AflIII        124  ACATGT               A^C[GA][CT]GT
Alw44I       1056  GTGCAC               G^TGCAC
AlwNI        1061  CAGTAACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI        1056  GTGCAC               G^TGCAC
ApoI          227  AAATTC               [GA]^AATT[CT]
Asp718I       828  GGTACC               G^GTACC
AvaI          232  CCCGAG               C^[CT]CG[GA]G
BanI          828  GGTACC               G^G[CT][GA]CC
BanII         582  GAGCTC               G[GA]GC[CT]^C
BbvI          283  GCAGC                GCAGC(8/12)
BclI           21  TGATCA               T^GATCA
BglII        1033  AGATCT               A^GATCT
BsaHI         479  GACGTC               G[GA]^CG[CT]C
BsaJI         233  CCGAGG               C^C[ACGT][ACGT]GG
BsgI           80  CTGCAC               GTGCAG(16/14)
BsmFI          97  GTCCC                GGGAC(10/14)
BsoBI         232  CCCGAG               C^[CT]CG[GA]G
BspHI         804  TCATGA               T^CATGA
BspLU11I      124  ACATGT               A^CATGT
BsrBI         596  GAGCGG               CCGCTC(-3/-3)
BstAPI        898  GCAGTTGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI        1033  AGATCT               [GA]^GATC[CT]
CfoI          354  GCGC                 GCG^C
Cfr13I        656  GGCCC                G^G[ACGT]CC
DraII         655  AGGCCCC              [GA]G^G[ACGT]CC[CT]
DraIII        614  CACAGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I      655  AGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoT22I       308  ATGCAT               ATGCA^T
FbaI           21  TGATCA               T^GATCA
Fnu4HI        283  GCAGC                GC^[ACGT]GC
HhaI          354  GCGC                 GCG^C
Hin1I         479  GACGTC               G[GA]^CG[CT]C
HinP1I        354  GCGC                 G^CGC
HpaI          681  GTTAAC               GTT^AAC
ItaI          283  GCAGC                GC^[ACGT]GC
KpnI          828  GGTACC               GGTAC^C
MflI         1033  AGATCT               [GA]^GATC[CT]
MslI          402  CACTGAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        767  CAGCTG               C[AC]G^C[GT]G
NsiI          308  ATGCAT               ATGCA^T
PstI          896  CTGCAG               CTGCA^G
PvuII         767  CAGCTG               CAG^CTG
RcaI          804  TCATGA               T^CATGA
SacI          582  GAGCTC               GAGCT^C
SalI          218  GTCGAC               G^TCGAC
Sau96I        656  GGCCC                G^G[ACGT]CC
SfcI          896  CTGCAG               C^T[GA][CT]AG
SmlI          277  CTCAAG               C^T[CT][GA]AG
SpeI          358  ACTAGT               A^CTAGT
StuI         1042  AGGCCT               AGG^CCT
TseI          283  GCAGC                G^C[AT]GC
Tsp45I        264  GTCAC                ^GT[GC]AC
XbaI         1036  TCTAGA               T^CTAGA
XhoII        1033  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AciI          204  GCGG                 CCGC(-3/-1)
              598  GCGG                
BbsI          727  GAAGAC               GAAGAC(2/6)
              645  GTCTTC              
BpuAI         727  GAAGAC               GAAGAC(2/6)
              645  GTCTTC              
BsiEI         966  CGATCG               CG[GA][CT]^CG
              979  CGATCG              
BsiYI         517  CCAAAGAAGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              814  CCATCTTCAGG         
BslI          517  CCAAAGAAGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              814  CCATCTTCAGG         
BsmAI         758  GAGAC                GTCTC(1/5)
              930  GAGAC               
BsrI          136  CCAGT                ACTGG(1/-1)
              872  CCAGT               
DdeI          538  CTTAG                C^T[ACGT]AG
              751  CTCAG               
HaeIII        656  GGCC                 GG^CC
             1043  GGCC                
HincII        218  GTCGAC               GT[CT]^[GA]AC
              681  GTTAAC              
HindII        218  GTCGAC               GT[CT]^[GA]AC
              681  GTTAAC              
MaeII         158  ACGT                 A^CGT
              480  ACGT                
MaeIII        264  GTCAC                ^GT[ACGT]AC
             1063  GTAAC               
NlaIV         656  GGCCCC               GG[ACGT]^[ACGT]CC
              828  GGTACC              
NspI           35  ACATGC               [GA]CATG^[CT]
              124  ACATGT              
PvuI          966  CGATCG               CGAT^CG
              979  CGATCG              
TfiI          808  GAATC                G^A[AT]TC
              862  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (   32 enzymes) #
#########################################

AfaI        AluI        AspHI       BfaI        BmyI        
BsiHKAI     Bsp1286I    BspDI       BstF5I      Cac8I       
ClaI        DpnII       FokI        HinfI       HphI        
MaeI        MboI        MboII       MnlI        MseI        
MwoI        NdeII       NlaIII      PleI        RsaI        
Sau3AI      SfaNI       TaqI        Tru9I       Tsp509I     
TspRI       TthHB8I     
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