Restriction map for
pF1KB8582
(Linear) (Six-base) MAPSORT of: KIBB8582.seq Check: 1930 from: 1 to: 2247
KIBB8582.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1169 1695 2247
Size: 1169 526 552
Fragments arranged by size:
1169 552 526
AccI GT'mk_AC
Cuts at: 0 1295 1457 2247
Size: 1295 162 790
Fragments arranged by size:
1295 790 162
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 273 439 1537 1681 2039 2247
Size: 247 26 166 1098 144 358 208
Fragments arranged by size:
1098 358 247 208 166 144 26
AhdI GACnn_n'nnGTC
Cuts at: 0 78 2247
Size: 78 2169
AleI CACnn'nnGTG
Cuts at: 0 1018 2247
Size: 1018 1229
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 715 718 1767 2247
Size: 715 3 1049 480
Fragments arranged by size:
1049 715 480 3
AlwNI CAG_nnn'CTG
Cuts at: 0 279 663 959 1716 1862 2193 2247
Size: 279 384 296 757 146 331 54
Fragments arranged by size:
757 384 331 296 279 146 54
ApaI G_GGCC'C
Cuts at: 0 1270 2247
Size: 1270 977
ApaLI G'TGCA_C
Cuts at: 0 1225 2247
Size: 1225 1022
ApoI r'AATT_y
Cuts at: 0 99 432 583 2247
Size: 99 333 151 1664
Fragments arranged by size:
1664 333 151 99
AvaI C'yCGr_G
Cuts at: 0 241 551 1395 1404 1823 2247
Size: 241 310 844 9 419 424
Fragments arranged by size:
844 424 419 310 241 9
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 511 544 2247
Size: 511 33 1703
Fragments arranged by size:
1703 511 33
BanI G'GyrC_C
Cuts at: 0 26 840 1048 1244 2247
Size: 26 814 208 196 1003
Fragments arranged by size:
1003 814 208 196 26
BanII G_rGCy'C
Cuts at: 0 169 766 1270 1405 1489 2247
Size: 169 597 504 135 84 758
Fragments arranged by size:
758 597 504 169 135 84
BbeI G_GCGC'C
Cuts at: 0 30 2247
Size: 30 2217
BbsI GAAGACnn'nnnn_
Cuts at: 0 1928 2096 2247
Size: 1928 168 151
Fragments arranged by size:
1928 168 151
BbvCI CC'TCA_GC
Cuts at: 0 572 949 2247
Size: 572 377 1298
Fragments arranged by size:
1298 572 377
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1352 1386 2247
Size: 1352 34 861
Fragments arranged by size:
1352 861 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2048 2247
Size: 2048 199
Bme1580I G_kGCm'C
Cuts at: 0 845 1053 1229 1270 1383 2247
Size: 845 208 176 41 113 864
Fragments arranged by size:
864 845 208 176 113 41
BmrI ACTGGGnnnn_n'
Cuts at: 0 920 2247
Size: 920 1327
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1056 2058 2247
Size: 1056 1002 189
Fragments arranged by size:
1056 1002 189
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 370 640 1123 1267 1459 1612 1915 2239
Size: 370 270 483 144 192 153 303 324
Cuts at: 2239 2247
Size: 8
Fragments arranged by size:
483 370 324 303 270 192 153 144
8
Bpu10I CC'TnA_GC
Cuts at: 0 572 949 1255 2068 2247
Size: 572 377 306 813 179
Fragments arranged by size:
813 572 377 306 179
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 868 982 2247
Size: 868 114 1265
Fragments arranged by size:
1265 868 114
BsaI GGTCTCn'nnnn_
Cuts at: 0 230 632 2141 2247
Size: 230 402 1509 106
Fragments arranged by size:
1509 402 230 106
BsaAI yAC'GTr
Cuts at: 0 1016 2247
Size: 1016 1231
BsaHI Gr'CG_yC
Cuts at: 0 27 1781 2247
Size: 27 1754 466
Fragments arranged by size:
1754 466 27
BsaWI w'CCGG_w
Cuts at: 0 147 2247
Size: 147 2100
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1534 1561 1564 1591 2247
Size: 1534 27 3 27 656
Fragments arranged by size:
1534 656 27 27 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 325 379 382 931 2122 2125 2134 2247
Size: 325 54 3 549 1191 3 9 113
Fragments arranged by size:
1191 549 325 113 54 9 3 3
BseYI C'CCAG_C
Cuts at: 0 1270 2247
Size: 1270 977
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1585 1729 2247
Size: 1585 144 518
Fragments arranged by size:
1585 518 144
BsiEI CG_ry'CG
Cuts at: 0 815 2247
Size: 815 1432
BsiHKAI G_wGCw'C
Cuts at: 0 206 910 1229 1261 1405 1489 2074 2247
Size: 206 704 319 32 144 84 585 173
Fragments arranged by size:
704 585 319 206 173 144 84 32
BsmI GAATG_Cn'
Cuts at: 0 607 1370 2247
Size: 607 763 877
Fragments arranged by size:
877 763 607
BsmBI CGTCTCn'nnnn_
Cuts at: 0 790 1788 2247
Size: 790 998 459
Fragments arranged by size:
998 790 459
Bsp1286I G_dGCh'C
Cuts at: 0 169 206 766 845 910 1053 1229 1261
Size: 169 37 560 79 65 143 176 32
Cuts at: 1261 1270 1383 1405 1489 2074 2247
Size: 9 113 22 84 585 173
Fragments arranged by size:
585 560 176 173 169 143 113 84
79 65 37 32 22 9
BspEI T'CCGG_A
Cuts at: 0 147 2247
Size: 147 2100
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1169 1443 1544 1607 1695 2247
Size: 1169 274 101 63 88 552
Fragments arranged by size:
1169 552 274 101 88 63
BsrBI CCG'CTC
Cuts at: 0 379 723 1914 2247
Size: 379 344 1191 333
Fragments arranged by size:
1191 379 344 333
BsrFI r'CCGG_y
Cuts at: 0 179 962 1700 2247
Size: 179 783 738 547
Fragments arranged by size:
783 738 547 179
BsrGI T'GTAC_A
Cuts at: 0 1007 1208 2247
Size: 1007 201 1039
Fragments arranged by size:
1039 1007 201
BstAPI GCAn_nnn'nTGC
Cuts at: 0 613 959 1224 1716 2247
Size: 613 346 265 492 531
Fragments arranged by size:
613 531 492 346 265
BstEII G'GTnAC_C
Cuts at: 0 621 2247
Size: 621 1626
BstXI CCAn_nnnn'nTGG
Cuts at: 0 777 2247
Size: 777 1470
BstYI r'GATC_y
Cuts at: 0 1886 2223 2247
Size: 1886 337 24
Fragments arranged by size:
1886 337 24
BtgI C'CryG_G
Cuts at: 0 455 2247
Size: 455 1792
BtsI GCAGTG_nn'
Cuts at: 0 874 1081 1227 1259 1340 1368 1614 1805
Size: 874 207 146 32 81 28 246 191
Cuts at: 1805 2247
Size: 442
Fragments arranged by size:
874 442 246 207 191 146 81 32
28
DraI TTT'AAA
Cuts at: 0 1992 2247
Size: 1992 255
EaeI y'GGCC_r
Cuts at: 0 173 542 767 812 1688 2247
Size: 173 369 225 45 876 559
Fragments arranged by size:
876 559 369 225 173 45
EagI C'GGCC_G
Cuts at: 0 812 2247
Size: 812 1435
EarI CTCTTCn'nnn_
Cuts at: 0 311 1240 1823 2015 2093 2247
Size: 311 929 583 192 78 154
Fragments arranged by size:
929 583 311 192 154 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 49 1132 2247
Size: 49 1083 1115
Fragments arranged by size:
1115 1083 49
EcoICRI GAG'CTC
Cuts at: 0 1403 1487 2247
Size: 1403 84 760
Fragments arranged by size:
1403 760 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 273 370 439 640 1123 1267 1459
Size: 247 26 97 69 201 483 144 192
Cuts at: 1459 1537 1612 1681 1915 2039 2239 2247
Size: 78 75 69 234 124 200 8
Fragments arranged by size:
483 247 234 201 200 192 144 124
97 78 75 69 69 26 8
EcoO109I rG'GnC_Cy
Cuts at: 0 1251 1267 1409 2063 2247
Size: 1251 16 142 654 184
Fragments arranged by size:
1251 654 184 142 16
EcoRI G'AATT_C
Cuts at: 0 432 2247
Size: 432 1815
FspI TGC'GCA
Cuts at: 0 1165 2247
Size: 1165 1082
HaeII r_GCGC'y
Cuts at: 0 30 2247
Size: 30 2217
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 64 70 96 102 202 234 2247
Size: 64 6 26 6 100 32 2013
Fragments arranged by size:
2013 100 64 32 26 6 6
HincII GTy'rAC
Cuts at: 0 1458 1533 2247
Size: 1458 75 714
Fragments arranged by size:
1458 714 75
HindIII A'AGCT_T
Cuts at: 0 733 2247
Size: 733 1514
KasI G'GCGC_C
Cuts at: 0 26 2247
Size: 26 2221
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 773 2247
Size: 773 1474
MscI TGG'CCA
Cuts at: 0 544 1690 2247
Size: 544 1146 557
Fragments arranged by size:
1146 557 544
MslI CAynn'nnrTG
Cuts at: 0 832 1018 1420 2247
Size: 832 186 402 827
Fragments arranged by size:
832 827 402 186
MspA1I CmG'CkG
Cuts at: 0 7 538 777 1359 1575 1659 1716 1893
Size: 7 531 239 582 216 84 57 177
Cuts at: 1893 2193 2247
Size: 300 54
Fragments arranged by size:
582 531 300 239 216 177 84 57
54 7
NaeI GCC'GGC
Cuts at: 0 181 964 1702 2247
Size: 181 783 738 545
Fragments arranged by size:
783 738 545 181
NarI GG'CG_CC
Cuts at: 0 27 2247
Size: 27 2220
NcoI C'CATG_G
Cuts at: 0 455 2247
Size: 455 1792
NgoMIV G'CCGG_C
Cuts at: 0 179 962 1700 2247
Size: 179 783 738 547
Fragments arranged by size:
783 738 547 179
NspI r_CATG'y
Cuts at: 0 879 2247
Size: 879 1368
PasI CC'CwG_GG
Cuts at: 0 2176 2199 2247
Size: 2176 23 48
Fragments arranged by size:
2176 48 23
PflMI CCAn_nnn'nTGG
Cuts at: 0 516 1290 2247
Size: 516 774 957
Fragments arranged by size:
957 774 516
PfoI T'CCnGG_A
Cuts at: 0 1436 1628 1673 2247
Size: 1436 192 45 574
Fragments arranged by size:
1436 574 192 45
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 310 342 2247
Size: 310 32 1905
Fragments arranged by size:
1905 310 32
PpuMI rG'GwC_Cy
Cuts at: 0 1251 2247
Size: 1251 996
PspOMI G'GGCC_C
Cuts at: 0 1266 2247
Size: 1266 981
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1729 1761 2247
Size: 1729 32 486
Fragments arranged by size:
1729 486 32
PstI C_TGCA'G
Cuts at: 0 263 707 1541 1658 1756 2189 2231 2247
Size: 263 444 834 117 98 433 42 16
Fragments arranged by size:
834 444 433 263 117 98 42 16
PvuII CAG'CTG
Cuts at: 0 7 538 777 1575 1659 1716 1893 2193
Size: 7 531 239 798 84 57 177 300
Cuts at: 2193 2247
Size: 54
Fragments arranged by size:
798 531 300 239 177 84 57 54
7
SacI G_AGCT'C
Cuts at: 0 1405 1489 2247
Size: 1405 84 758
Fragments arranged by size:
1405 758 84
SalI G'TCGA_C
Cuts at: 0 1456 2247
Size: 1456 791
SbfI CC_TGCA'GG
Cuts at: 0 263 1541 2247
Size: 263 1278 706
Fragments arranged by size:
1278 706 263
SfcI C'TryA_G
Cuts at: 0 259 703 1537 1654 1752 1854 2185 2227
Size: 259 444 834 117 98 102 331 42
Cuts at: 2227 2247
Size: 20
Fragments arranged by size:
834 444 331 259 117 102 98 42
20
SfoI GGC'GCC
Cuts at: 0 28 2247
Size: 28 2219
SmaI CCC'GGG
Cuts at: 0 1825 2247
Size: 1825 422
SmlI C'TyrA_G
Cuts at: 0 883 997 2247
Size: 883 114 1250
Fragments arranged by size:
1250 883 114
SphI G_CATG'C
Cuts at: 0 879 2247
Size: 879 1368
StyI C'CwwG_G
Cuts at: 0 455 984 1289 2247
Size: 455 529 305 958
Fragments arranged by size:
958 529 455 305
TatI w'GTAC_w
Cuts at: 0 1007 1208 1850 2247
Size: 1007 201 642 397
Fragments arranged by size:
1007 642 397 201
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 723 755 2247
Size: 723 32 1492
Fragments arranged by size:
1492 723 32
Tth111I GACn'n_nGTC
Cuts at: 0 403 2247
Size: 403 1844
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1315 2247
Size: 1315 932
XmaI C'CCGG_G
Cuts at: 0 1823 2247
Size: 1823 424
XmnI GAAnn'nnTTC
Cuts at: 0 1666 2247
Size: 1666 581
Enzymes that do cut:
AarI AccI AcuI AhdI AleI AlfI AlwNI ApaI
ApaLI ApoI AvaI BaeI BanI BanII BbeI BbsI
BbvCI BcgI BciVI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI
BsrBI BsrFI BsrGI BstAPI BstEII BstXI BstYI BtgI
BtsI DraI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII KasI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PflMI PfoI PpiI PpuMI PspOMI PsrI
PstI PvuII SacI SalI SbfI SfcI SfoI SmaI
SmlI SphI StyI TatI TstI Tth111I XcmI XmaI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AloI
AscI AseI AsiSI AvrII BamHI BclI BfrBI BglI
BglII BlpI BmgBI BmtI BsaBI BsiWI BspHI BsrDI
BssHII BssSI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI
DraIII DrdI EcoNI EcoRV FalI FseI FspAI HpaI
KpnI MfeI MluI NdeI NheI NotI NruI NsiI
PacI PciI PmeI PmlI PshAI PsiI PspXI PvuI
RsrII SacII SanDI SapI ScaI SexAI SfiI SgfI
SgrAI SnaBI SpeI SrfI SspI StuI SwaI TaqII
XbaI XhoI ZraI