Restriction map for
pF1KB8571
(Circular) (Six-base) MAPSORT of: pF1KB8571.seq Check: 9108 from: 1 to: 4832
pF1KB8571 4832 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AatII G_ACGT'C
Cuts at: 1725 1725
Size: 4832
AccI GT'mk_AC
Cuts at: 1870 1870
Size: 4832
Acc65I G'GTAC_C
Cuts at: 1848 1848
Size: 4832
AclI AA'CG_TT
Cuts at: 4672 4672
Size: 4832
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 590 2657 3089 3442 4104 590
Size: 2067 432 353 662 1318
Fragments arranged by size:
2067 1318 662 432 353
AflIII A'CryG_T
Cuts at: 1024 1816 2184 3984 4265 1024
Size: 792 368 1800 281 1591
Fragments arranged by size:
1800 1591 792 368 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1690 1690
Size: 4832
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 919 951 919
Size: 32 4800
AlwNI CAG_nnn'CTG
Cuts at: 613 910 1459 2137 3575 613
Size: 297 549 678 1438 1870
Fragments arranged by size:
1870 1438 678 549 297
ApaLI G'TGCA_C
Cuts at: 3670 3670
Size: 4832
ApoI r'AATT_y
Cuts at: 339 1836 4185 4441 339
Size: 1497 2349 256 730
Fragments arranged by size:
2349 1497 730 256
AseI AT'TA_AT
Cuts at: 20 1315 20
Size: 1295 3537
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4832
AvaI C'yCGr_G
Cuts at: 1327 1852 1327
Size: 525 4307
BamHI G'GATC_C
Cuts at: 1857 1857
Size: 4832
BanI G'GyrC_C
Cuts at: 1388 1848 2262 2510 2545 1388
Size: 460 414 248 35 3675
Fragments arranged by size:
3675 460 414 248 35
BanII G_rGCy'C
Cuts at: 964 1352 1399 1435 1511 1846 2876 964
Size: 388 47 36 76 335 1030 2920
Fragments arranged by size:
2920 1030 388 335 76 47 36
BbeI G_GCGC'C
Cuts at: 2266 2514 2266
Size: 248 4584
BbsI GAAGACnn'nnnn_
Cuts at: 385 1474 1795 385
Size: 1089 321 3422
Fragments arranged by size:
3422 1089 321
BciVI GTATCCnnnnn_n'
Cuts at: 1681 2730 3786 4832 1681
Size: 1049 1056 1046 1681
Fragments arranged by size:
1681 1056 1049 1046
BclI T'GATC_A
Cuts at: 219 2352 219
Size: 2133 2699
BfrBI ATG'CAT
Cuts at: 1775 1775
Size: 4832
BglII A'GATC_T
Cuts at: 2347 4384 2347
Size: 2037 2795
BlpI GC'TnA_GC
Cuts at: 1944 1944
Size: 4832
Bme1580I G_kGCm'C
Cuts at: 2457 2550 3674 2457
Size: 93 1124 3615
Fragments arranged by size:
3615 1124 93
BmgBI CAC'GTC
Cuts at: 1025 1025
Size: 4832
BmrI ACTGGGnnnn_n'
Cuts at: 1394 1805 2044 2125 2460 4604 1394
Size: 411 239 81 335 2144 1622
Fragments arranged by size:
2144 1622 411 335 239 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 561 3199 561
Size: 2638 2194
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 635 902 1366 1513 1731 635
Size: 267 464 147 218 3736
Fragments arranged by size:
3736 464 267 218 147
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1130 2008 2887 3601 3899 1130
Size: 878 879 714 298 2063
Fragments arranged by size:
2063 879 878 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4536 4536
Size: 4832
BsaAI yAC'GTr
Cuts at: 259 2185 259
Size: 1926 2906
BsaBI GATnn'nnATC
Cuts at: 6 171 2346 4383 6
Size: 165 2175 2037 455
Fragments arranged by size:
2175 2037 455 165
BsaHI Gr'CG_yC
Cuts at: 1722 2263 2511 3213 4709 1722
Size: 541 248 702 1496 1845
Fragments arranged by size:
1845 1496 702 541 248
BsaWI w'CCGG_w
Cuts at: 2021 2542 3220 3631 3778 4052 2021
Size: 521 678 411 147 274 2801
Fragments arranged by size:
2801 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 900 930 1752 1782 900
Size: 30 822 30 3950
Fragments arranged by size:
3950 822 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1415 1415
Size: 4832
BseYI C'CCAG_C
Cuts at: 1412 2257 3015 3680 4088 1412
Size: 845 758 665 408 2156
Fragments arranged by size:
2156 845 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 2374 2420 3650 104
Size: 2270 46 1230 1286
Fragments arranged by size:
2270 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 964 1511 1762 1846 2624 2814 3674 964
Size: 547 251 84 778 190 860 2122
Fragments arranged by size:
2122 860 778 547 251 190 84
BsmI GAATG_Cn'
Cuts at: 1569 1569
Size: 4832
BsmBI CGTCTCn'nnnn_
Cuts at: 1015 1180 1015
Size: 165 4667
Bsp1286I G_dGCh'C
Cuts at: 964 1352 1399 1435 1511 1762 1846 2457 2550
Size: 388 47 36 76 251 84 611 93
Cuts at: 2550 2624 2814 2876 3674 964
Size: 74 190 62 798 2122
Fragments arranged by size:
2122 798 611 388 251 190 93 84
76 74 62 47 36
BspHI T'CATG_A
Cuts at: 1088 3264 1088
Size: 2176 2656
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1882 2096 2398 2779 1882
Size: 214 302 381 3935
Fragments arranged by size:
3935 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1808 3124 3178 4662 4827 50
Size: 1758 1316 54 1484 165 55
Fragments arranged by size:
1758 1484 1316 165 55 54
BsrDI GCAATG_nn'
Cuts at: 855 1574 2744 855
Size: 719 1170 2943
Fragments arranged by size:
2943 1170 719
BsrFI r'CCGG_y
Cuts at: 2830 3011 2830
Size: 181 4651
BsrGI T'GTAC_A
Cuts at: 696 696
Size: 4832
BssSI C'ACGA_G
Cuts at: 3103 3811 3103
Size: 708 4124
BstAPI GCAn_nnn'nTGC
Cuts at: 2209 2209
Size: 4832
BstBI TT'CG_AA
Cuts at: 3193 3193
Size: 4832
BstEII G'GTnAC_C
Cuts at: 1519 1519
Size: 4832
BstYI r'GATC_y
Cuts at: 1 1857 2324 2347 2682 2928 3234 3246 3332
Size: 1856 467 23 335 246 306 12 86
Cuts at: 3332 4384 1
Size: 1052 449
Fragments arranged by size:
1856 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 1095 1095
Size: 4832
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2782 2963 3126 2782
Size: 181 163 4488
Fragments arranged by size:
4488 181 163
BtsI GCAGTG_nn'
Cuts at: 388 2075 388
Size: 1687 3145
DraI TTT'AAA
Cuts at: 162 1831 162
Size: 1669 3163
DraIII CAC_nnn'GTG
Cuts at: 1388 1388
Size: 4832
DrdI GACnn_nn'nnGTC
Cuts at: 2538 3882 2538
Size: 1344 3488
EaeI y'GGCC_r
Cuts at: 2417 2591 2982 3009 4354 2417
Size: 174 391 27 1345 2895
Fragments arranged by size:
2895 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2417 2417
Size: 4832
EarI CTCTTCn'nnn_
Cuts at: 1478 1645 1684 1750 2855 3065 1478
Size: 167 39 66 1105 210 3245
Fragments arranged by size:
3245 1105 210 167 66 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3040 3782 3928 4642 3040
Size: 742 146 714 3230
Fragments arranged by size:
3230 742 714 146
EcoICRI GAG'CTC
Cuts at: 962 1509 1844 962
Size: 547 335 3950
Fragments arranged by size:
3950 547 335
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 590 635 902 1366 1513 1731 2657 3089 3442
Size: 45 267 464 147 218 926 432 353
Cuts at: 3442 4104 590
Size: 662 1318
Fragments arranged by size:
1318 926 662 464 432 353 267 218
147 45
EcoNI CCTnn'n_nnAGG
Cuts at: 581 581
Size: 4832
EcoO109I rG'GnC_Cy
Cuts at: 1971 1971
Size: 4832
EcoRI G'AATT_C
Cuts at: 1836 1836
Size: 4832
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2851 2851
Size: 4832
FspI TGC'GCA
Cuts at: 2613 2613
Size: 4832
HaeII r_GCGC'y
Cuts at: 2266 2514 3744 4515 2266
Size: 248 1230 771 2583
Fragments arranged by size:
2583 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 102 134 865 897 1339 1371 1679 1711 1792
Size: 32 731 32 442 32 308 32 81
Cuts at: 1792 1824 2674 2704 2706 2736 102
Size: 32 850 30 2 30 2198
Fragments arranged by size:
2198 850 731 442 308 81 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1871 1871
Size: 4832
KasI G'GCGC_C
Cuts at: 2262 2510 2262
Size: 248 4584
KpnI G_GTAC'C
Cuts at: 1852 1852
Size: 4832
MluI A'CGCG_T
Cuts at: 4265 4265
Size: 4832
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 480 1540 3591 3775 480
Size: 1060 2051 184 1537
Fragments arranged by size:
2051 1537 1060 184
MscI TGG'CCA
Cuts at: 2593 2593
Size: 4832
MslI CAynn'nnrTG
Cuts at: 1100 1292 1672 1780 2821 2948 1100
Size: 192 380 108 1041 127 2984
Fragments arranged by size:
2984 1041 380 192 127 108
MspA1I CmG'CkG
Cuts at: 397 1456 1481 1943 2257 2617 3401 3646 397
Size: 1059 25 462 314 360 784 245 1583
Fragments arranged by size:
1583 1059 784 462 360 314 245 25
NaeI GCC'GGC
Cuts at: 3013 3013
Size: 4832
NarI GG'CG_CC
Cuts at: 2263 2511 2263
Size: 248 4584
NcoI C'CATG_G
Cuts at: 1095 1095
Size: 4832
NgoMIV G'CCGG_C
Cuts at: 3011 3011
Size: 4832
NsiI A_TGCA'T
Cuts at: 1777 1777
Size: 4832
NspI r_CATG'y
Cuts at: 1820 1885 2385 3988 1820
Size: 65 500 1603 2664
Fragments arranged by size:
2664 1603 500 65
PacI TTA_AT'TAA
Cuts at: 296 296
Size: 4832
PasI CC'CwG_GG
Cuts at: 1011 1599 1011
Size: 588 4244
PciI A'CATG_T
Cuts at: 1816 3984 1816
Size: 2168 2664
PflMI CCAn_nnn'nTGG
Cuts at: 400 984 1777 400
Size: 584 793 3455
Fragments arranged by size:
3455 793 584
PmeI GTTT'AAAC
Cuts at: 1831 1831
Size: 4832
PmlI CAC'GTG
Cuts at: 259 259
Size: 4832
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3278 3310 3278
Size: 32 4800
PstI C_TGCA'G
Cuts at: 1455 1879 2564 1455
Size: 424 685 3723
Fragments arranged by size:
3723 685 424
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4832
PvuII CAG'CTG
Cuts at: 397 1456 1481 2257 2617 397
Size: 1059 25 776 360 2612
Fragments arranged by size:
2612 1059 776 360 25
RsrII CG'GwC_CG
Cuts at: 3027 3027
Size: 4832
SacI G_AGCT'C
Cuts at: 964 1511 1846 964
Size: 547 335 3950
Fragments arranged by size:
3950 547 335
SalI G'TCGA_C
Cuts at: 1869 1869
Size: 4832
SapI GCTCTTCn'nnn_
Cuts at: 1684 1750 2855 3065 1684
Size: 66 1105 210 3451
Fragments arranged by size:
3451 1105 210 66
SbfI CC_TGCA'GG
Cuts at: 1879 1879
Size: 4832
ScaI AGT'ACT
Cuts at: 4349 4349
Size: 4832
SfcI C'TryA_G
Cuts at: 33 1451 1875 2560 3528 3719 33
Size: 1418 424 685 968 191 1146
Fragments arranged by size:
1418 1146 968 685 424 191
SfoI GGC'GCC
Cuts at: 2264 2512 2264
Size: 248 4584
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4832
SmaI CCC'GGG
Cuts at: 1854 1854
Size: 4832
SmlI C'TyrA_G
Cuts at: 1145 1987 2902 3616 3878 1145
Size: 842 915 714 262 2099
Fragments arranged by size:
2099 915 842 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4832
SphI G_CATG'C
Cuts at: 1885 2385 1885
Size: 500 4332
SspI AAT'ATT
Cuts at: 673 673
Size: 4832
StyI C'CwwG_G
Cuts at: 515 1095 1524 1966 2320 515
Size: 580 429 442 354 3027
Fragments arranged by size:
3027 580 442 429 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 138 1376 2415 2548 3007 3216 138
Size: 1238 1039 133 459 209 1754
Fragments arranged by size:
1754 1238 1039 459 209 133
TatI w'GTAC_w
Cuts at: 696 4347 696
Size: 3651 1181
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1520 1552 1752 1784 4349 4381 1520
Size: 32 200 32 2565 32 1971
Fragments arranged by size:
2565 1971 200 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2629 2629
Size: 4832
XbaI T'CTAG_A
Cuts at: 1863 1863
Size: 4832
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2327 2327
Size: 4832
XmaI C'CCGG_G
Cuts at: 1852 1852
Size: 4832
XmnI GAAnn'nnTTC
Cuts at: 672 672
Size: 4832
ZraI GAC'GTC
Cuts at: 1723 1723
Size: 4832
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AlfI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PacI
PasI PciI PflMI PmeI PmlI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AhdI AleI ApaI AscI
AvrII BaeI BbvCI BcgI BglI BmtI Bpu10I BsgI
BsiWI BspEI BssHII BstXI BstZ17I Bsu36I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI PfoI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI