Restriction map for
pF1KB8570
(Circular) (Six-base) MAPSORT of: pF1KB8570.seq Check: 525 from: 1 to: 5582
pF1KB8570 5582 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 170 728 2620 170
Size: 558 1892 3132
Fragments arranged by size:
3132 1892 558
Acc65I G'GTAC_C
Cuts at: 2598 2598
Size: 5582
AclI AA'CG_TT
Cuts at: 5422 5422
Size: 5582
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1145 1193 1426 3407 3839 4192 4854 1145
Size: 48 233 1981 432 353 662 1873
Fragments arranged by size:
1981 1873 662 432 353 233 48
AfeI AGC'GCT
Cuts at: 259 259
Size: 5582
AflII C'TTAA_G
Cuts at: 763 1735 763
Size: 972 4610
AflIII A'CryG_T
Cuts at: 794 2934 4734 5015 794
Size: 2140 1800 281 1361
Fragments arranged by size:
2140 1800 1361 281
AleI CACnn'nnGTG
Cuts at: 1126 1126
Size: 5582
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 329 329
Size: 5582
AlwNI CAG_nnn'CTG
Cuts at: 2887 4325 2887
Size: 1438 4144
ApaLI G'TGCA_C
Cuts at: 575 4420 575
Size: 3845 1737
ApoI r'AATT_y
Cuts at: 655 845 857 2116 2586 4935 5191 655
Size: 190 12 1259 470 2349 256 1046
Fragments arranged by size:
2349 1259 1046 470 256 190 12
AseI AT'TA_AT
Cuts at: 20 20
Size: 5582
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5582
AvaI C'yCGr_G
Cuts at: 1012 2602 1012
Size: 1590 3992
BamHI G'GATC_C
Cuts at: 2607 2607
Size: 5582
BanI G'GyrC_C
Cuts at: 475 2598 3012 3260 3295 475
Size: 2123 414 248 35 2762
Fragments arranged by size:
2762 2123 414 248 35
BanII G_rGCy'C
Cuts at: 157 2596 3626 157
Size: 2439 1030 2113
Fragments arranged by size:
2439 2113 1030
BbeI G_GCGC'C
Cuts at: 479 3016 3264 479
Size: 2537 248 2797
Fragments arranged by size:
2797 2537 248
BbsI GAAGACnn'nnnn_
Cuts at: 774 774
Size: 5582
BbvCI CC'TCA_GC
Cuts at: 1385 1385
Size: 5582
BciVI GTATCCnnnnn_n'
Cuts at: 178 1391 3480 4536 5582 178
Size: 1213 2089 1056 1046 178
Fragments arranged by size:
2089 1213 1056 1046 178
BclI T'GATC_A
Cuts at: 2475 3102 2475
Size: 627 4955
BglI GCCn_nnn'nGGC
Cuts at: 137 137
Size: 5582
BglII A'GATC_T
Cuts at: 3097 5134 3097
Size: 2037 3545
BlpI GC'TnA_GC
Cuts at: 2694 2694
Size: 5582
Bme1580I G_kGCm'C
Cuts at: 579 3207 3300 4424 579
Size: 2628 93 1124 1737
Fragments arranged by size:
2628 1737 1124 93
BmgBI CAC'GTC
Cuts at: 168 168
Size: 5582
BmrI ACTGGGnnnn_n'
Cuts at: 2794 2875 3210 5354 2794
Size: 81 335 2144 3022
Fragments arranged by size:
3022 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 561 2176 3949 561
Size: 1615 1773 2194
Fragments arranged by size:
2194 1773 1615
Bpu10I CC'TnA_GC
Cuts at: 1385 2203 1385
Size: 818 4764
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 773 1834 2758 3637 4351 4649 773
Size: 1061 924 879 714 298 1706
Fragments arranged by size:
1706 1061 924 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 275 5286 275
Size: 5011 571
BsaAI yAC'GTr
Cuts at: 2935 2935
Size: 5582
BsaBI GATnn'nnATC
Cuts at: 6 3096 5133 6
Size: 3090 2037 455
Fragments arranged by size:
3090 2037 455
BsaHI Gr'CG_yC
Cuts at: 242 290 476 3013 3261 3963 5459 242
Size: 48 186 2537 248 702 1496 365
Fragments arranged by size:
2537 1496 702 365 248 186 48
BsaWI w'CCGG_w
Cuts at: 181 1823 2771 3292 3970 4381 4528 4802 181
Size: 1642 948 521 678 411 147 274 961
Fragments arranged by size:
1642 961 948 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2513 2543 2513
Size: 30 5552
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2537 2537
Size: 5582
BseYI C'CCAG_C
Cuts at: 3007 3765 4430 4838 3007
Size: 758 665 408 3751
Fragments arranged by size:
3751 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 3124 3170 4400 104
Size: 3020 46 1230 1286
Fragments arranged by size:
3020 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 544 579 2596 3374 3564 4424 544
Size: 35 2017 778 190 860 1702
Fragments arranged by size:
2017 1702 860 778 190 35
BsmI GAATG_Cn'
Cuts at: 1819 1819
Size: 5582
BsmBI CGTCTCn'nnnn_
Cuts at: 696 696
Size: 5582
Bsp1286I G_dGCh'C
Cuts at: 157 544 579 2596 3207 3300 3374 3564 3626
Size: 387 35 2017 611 93 74 190 62
Cuts at: 3626 4424 157
Size: 798 1315
Fragments arranged by size:
2017 1315 798 611 387 190 93 74
62 35
BspEI T'CCGG_A
Cuts at: 181 181
Size: 5582
BspHI T'CATG_A
Cuts at: 1617 4014 1617
Size: 2397 3185
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2632 2846 3148 3529 2632
Size: 214 302 381 4685
Fragments arranged by size:
4685 381 302 214
BsrBI CCG'CTC
Cuts at: 50 278 3874 3928 5412 5577 50
Size: 228 3596 54 1484 165 55
Fragments arranged by size:
3596 1484 228 165 55 54
BsrDI GCAATG_nn'
Cuts at: 432 958 3494 432
Size: 526 2536 2520
Fragments arranged by size:
2536 2520 526
BsrFI r'CCGG_y
Cuts at: 3580 3761 3580
Size: 181 5401
BsrGI T'GTAC_A
Cuts at: 1170 1472 1693 2031 2190 2340 1170
Size: 302 221 338 159 150 4412
Fragments arranged by size:
4412 338 302 221 159 150
BssHII G'CGCG_C
Cuts at: 268 268
Size: 5582
BssSI C'ACGA_G
Cuts at: 2018 2434 3853 4561 2018
Size: 416 1419 708 3039
Fragments arranged by size:
3039 1419 708 416
BstAPI GCAn_nnn'nTGC
Cuts at: 1669 2959 1669
Size: 1290 4292
BstBI TT'CG_AA
Cuts at: 3943 3943
Size: 5582
BstYI r'GATC_y
Cuts at: 1 215 1039 1134 2607 3074 3097 3432 3678
Size: 214 824 95 1473 467 23 335 246
Cuts at: 3678 3984 3996 4082 5134 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1473 1052 824 467 449 335 306 246
214 95 86 23 12
BstZ17I GTA'TAC
Cuts at: 729 729
Size: 5582
BtgI C'CryG_G
Cuts at: 245 570 245
Size: 325 5257
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3532 3713 3876 3532
Size: 181 163 5238
Fragments arranged by size:
5238 181 163
BtsI GCAGTG_nn'
Cuts at: 509 1009 2825 509
Size: 500 1816 3266
Fragments arranged by size:
3266 1816 500
DraI TTT'AAA
Cuts at: 2483 2581 2483
Size: 98 5484
DrdI GACnn_nn'nnGTC
Cuts at: 3288 4632 3288
Size: 1344 4238
EaeI y'GGCC_r
Cuts at: 339 3167 3341 3732 3759 5104 339
Size: 2828 174 391 27 1345 817
Fragments arranged by size:
2828 1345 817 391 174 27
EagI C'GGCC_G
Cuts at: 3167 3167
Size: 5582
EarI CTCTTCn'nnn_
Cuts at: 437 1211 1406 2294 2438 3605 3815 437
Size: 774 195 888 144 1167 210 2204
Fragments arranged by size:
2204 1167 888 774 210 195 144
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3790 4532 4678 5392 3790
Size: 742 146 714 3980
Fragments arranged by size:
3980 742 714 146
EcoICRI GAG'CTC
Cuts at: 2594 2594
Size: 5582
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1145 1193 1426 3407 3839 4192 4854 1145
Size: 48 233 1981 432 353 662 1873
Fragments arranged by size:
1981 1873 662 432 353 233 48
EcoO109I rG'GnC_Cy
Cuts at: 1087 2721 1087
Size: 1634 3948
EcoRI G'AATT_C
Cuts at: 2586 2586
Size: 5582
EcoRV GAT'ATC
Cuts at: 498 498
Size: 5582
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3601 3601
Size: 5582
FspI TGC'GCA
Cuts at: 3363 3363
Size: 5582
HaeII r_GCGC'y
Cuts at: 261 479 3016 3264 4494 5265 261
Size: 218 2537 248 1230 771 578
Fragments arranged by size:
2537 1230 771 578 248 218
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 176 208 1006 1038 1312 1344 1558 1590 1912
Size: 32 798 32 274 32 214 32 322
Cuts at: 1912 1944 2295 2327 3424 3454 3456 3486 176
Size: 32 351 32 1097 30 2 30 2272
Fragments arranged by size:
2272 1097 798 351 322 274 214 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 369 2621 369
Size: 2252 3330
HpaI GTT'AAC
Cuts at: 369 369
Size: 5582
KasI G'GCGC_C
Cuts at: 475 3012 3260 475
Size: 2537 248 2797
Fragments arranged by size:
2797 2537 248
KpnI G_GTAC'C
Cuts at: 2602 2602
Size: 5582
MfeI C'AATT_G
Cuts at: 2573 2573
Size: 5582
MluI A'CGCG_T
Cuts at: 5015 5015
Size: 5582
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 885 2343 4341 4525 885
Size: 1458 1998 184 1942
Fragments arranged by size:
1998 1942 1458 184
MscI TGG'CCA
Cuts at: 341 3343 341
Size: 3002 2580
MslI CAynn'nnrTG
Cuts at: 1126 1616 3571 3698 1126
Size: 490 1955 127 3010
Fragments arranged by size:
3010 1955 490 127
MspA1I CmG'CkG
Cuts at: 229 247 2693 3007 3367 4151 4396 229
Size: 18 2446 314 360 784 245 1415
Fragments arranged by size:
2446 1415 784 360 314 245 18
NaeI GCC'GGC
Cuts at: 3763 3763
Size: 5582
NarI GG'CG_CC
Cuts at: 476 3013 3261 476
Size: 2537 248 2797
Fragments arranged by size:
2797 2537 248
NcoI C'CATG_G
Cuts at: 570 570
Size: 5582
NgoMIV G'CCGG_C
Cuts at: 3761 3761
Size: 5582
NspI r_CATG'y
Cuts at: 688 798 2635 3135 4738 688
Size: 110 1837 500 1603 1532
Fragments arranged by size:
1837 1603 1532 500 110
PasI CC'CwG_GG
Cuts at: 150 150
Size: 5582
PciI A'CATG_T
Cuts at: 794 4734 794
Size: 3940 1642
PfoI T'CCnGG_A
Cuts at: 194 194
Size: 5582
PmeI GTTT'AAAC
Cuts at: 2581 2581
Size: 5582
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4028 4060 4028
Size: 32 5550
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1181 1213 1181
Size: 32 5550
PstI C_TGCA'G
Cuts at: 212 927 2629 3314 212
Size: 715 1702 685 2480
Fragments arranged by size:
2480 1702 715 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5582
PvuII CAG'CTG
Cuts at: 229 3007 3367 229
Size: 2778 360 2444
Fragments arranged by size:
2778 2444 360
RsrII CG'GwC_CG
Cuts at: 3777 3777
Size: 5582
SacI G_AGCT'C
Cuts at: 2596 2596
Size: 5582
SacII CC_GC'GG
Cuts at: 248 248
Size: 5582
SalI G'TCGA_C
Cuts at: 2619 2619
Size: 5582
SapI GCTCTTCn'nnn_
Cuts at: 1211 1406 3605 3815 1211
Size: 195 2199 210 2978
Fragments arranged by size:
2978 2199 210 195
SbfI CC_TGCA'GG
Cuts at: 2629 2629
Size: 5582
ScaI AGT'ACT
Cuts at: 5099 5099
Size: 5582
SexAI A'CCwGG_T
Cuts at: 741 741
Size: 5582
SfcI C'TryA_G
Cuts at: 33 208 923 2625 3310 4278 4469 33
Size: 175 715 1702 685 968 191 1146
Fragments arranged by size:
1702 1146 968 715 685 191 175
SfoI GGC'GCC
Cuts at: 477 3014 3262 477
Size: 2537 248 2797
Fragments arranged by size:
2797 2537 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5582
SmaI CCC'GGG
Cuts at: 2604 2604
Size: 5582
SmlI C'TyrA_G
Cuts at: 763 788 1735 1849 2737 3652 4366 4628 763
Size: 25 947 114 888 915 714 262 1717
Fragments arranged by size:
1717 947 915 888 714 262 114 25
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5582
SphI G_CATG'C
Cuts at: 2635 3135 2635
Size: 500 5082
SspI AAT'ATT
Cuts at: 774 2551 774
Size: 1777 3805
StyI C'CwwG_G
Cuts at: 570 2716 3070 570
Size: 2146 354 3082
Fragments arranged by size:
3082 2146 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3165 3298 3757 3966 3165
Size: 133 459 209 4781
Fragments arranged by size:
4781 459 209 133
TatI w'GTAC_w
Cuts at: 1155 1170 1324 1472 1693 2031 2190 2340 5097
Size: 15 154 148 221 338 159 150 2757
Cuts at: 5097 1155
Size: 1640
Fragments arranged by size:
2757 1640 338 221 159 154 150 148
15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5099 5131 5099
Size: 32 5550
Tth111I GACn'n_nGTC
Cuts at: 166 3379 166
Size: 3213 2369
XbaI T'CTAG_A
Cuts at: 1939 2563 2613 1939
Size: 624 50 4908
Fragments arranged by size:
4908 624 50
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3077 3077
Size: 5582
XmaI C'CCGG_G
Cuts at: 2602 2602
Size: 5582
XmnI GAAnn'nnTTC
Cuts at: 115 115
Size: 5582
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstYI BstZ17I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PfoI PmeI PpiI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AgeI AhdI AloI ApaI AscI AvrII
BaeI BcgI BfrBI BmtI BpmI BsgI BsiWI BstEII
BstXI Bsu36I ClaI CspCI DraIII EcoNI FseI FspAI
HindIII NdeI NheI NotI NruI NsiI PacI PflMI
PmlI PpuMI PshAI PsiI PspOMI PspXI SanDI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI ZraI