Restriction map for pF1KB8568
 (Linear) (Six-base) MAPSORT of: KIBB8568.seq  Check: 6015  from: 1  to: 2355



KIBB8568.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:06  ..

AccI GT'mk_AC

Cuts at:      0     282    2355 
   Size:        282    2073
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     271     791    1154    1463    1559    1595    2279    2294 
   Size:        271     520     363     309      96      36     684      15

Cuts at:   2294    2355 
   Size:         61

  Fragments arranged by size: 

                 684     520     363     309     271      96      61      36
                  15
 
AflIII A'CryG_T

Cuts at:      0    1312    2355 
   Size:       1312    1043
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1799    2355 
   Size:       1799     556
 
ApoI r'AATT_y

Cuts at:      0     826    1060    1418    1833    2097    2195    2292    2355 
   Size:        826     234     358     415     264      98      97      63

  Fragments arranged by size: 

                 826     415     358     264     234      98      97      63
            
 
AseI AT'TA_AT

Cuts at:      0    2171    2355 
   Size:       2171     184
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     188     779    1453    2355 
   Size:        188     591     674     902

  Fragments arranged by size: 

                 902     674     591     188
 
BclI T'GATC_A

Cuts at:      0     171    2355 
   Size:        171    2184
 
BfrBI ATG'CAT

Cuts at:      0    1749    2355 
   Size:       1749     606
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2129    2355 
   Size:       2129     226
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     341     460    1178    1409    2355 
   Size:        341     119     718     231     946

  Fragments arranged by size: 

                 946     718     341     231     119
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     110    2355 
   Size:        110    2245
 
BsaAI yAC'GTr

Cuts at:      0    2205    2355 
   Size:       2205     150
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2310    2340    2355 
   Size:       2310      30      15

  Fragments arranged by size: 

                2310      30      15
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1420    1900    2355 
   Size:       1420     480     455

  Fragments arranged by size: 

                1420     480     455
 
BseYI C'CCAG_C

Cuts at:      0    1045    2355 
   Size:       1045    1310
 
BsiHKAI G_wGCw'C

Cuts at:      0    1705    2355 
   Size:       1705     650
 
BsmI GAATG_Cn'

Cuts at:      0     765    1049    2355 
   Size:        765     284    1306

  Fragments arranged by size: 

                1306     765     284
 
Bsp1286I G_dGCh'C

Cuts at:      0    1705    2355 
   Size:       1705     650
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     266    2355 
   Size:        266    2089
 
BstZ17I GTA'TAC

Cuts at:      0     283    2355 
   Size:        283    2072
 
BtsI GCAGTG_nn'

Cuts at:      0    1781    2355 
   Size:       1781     574
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1883    1918    2355 
   Size:       1883      35     437

  Fragments arranged by size: 

                1883     437      35
 
DraI TTT'AAA

Cuts at:      0     315     780    2355 
   Size:        315     465    1575

  Fragments arranged by size: 

                1575     465     315
 
EarI CTCTTCn'nnn_

Cuts at:      0     211     875    1130    1341    2270    2355 
   Size:        211     664     255     211     929      85

  Fragments arranged by size: 

                 929     664     255     211     211      85
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     271     341     460     791    1154    1178    1409    1463 
   Size:        271      70     119     331     363      24     231      54

Cuts at:   1463    1559    1595    2279    2294    2355 
   Size:         96      36     684      15      61

  Fragments arranged by size: 

                 684     363     331     271     231     119      96      70
                  61      54      36      24      15
 
EcoO109I rG'GnC_Cy

Cuts at:      0    2123    2355 
   Size:       2123     232
 
EcoRI G'AATT_C

Cuts at:      0    2292    2355 
   Size:       2292      63
 
EcoRV GAT'ATC

Cuts at:      0     583    2355 
   Size:        583    1772
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1408    2355 
   Size:       1408     947
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1644    1676    2355 
   Size:       1644      32     679

  Fragments arranged by size: 

                1644     679      32
 
HincII GTy'rAC

Cuts at:      0    1845    2355 
   Size:       1845     510
 
HindIII A'AGCT_T

Cuts at:      0     143    2214    2355 
   Size:        143    2071     141

  Fragments arranged by size: 

                2071     143     141
 
MluI A'CGCG_T

Cuts at:      0    1312    2355 
   Size:       1312    1043
 
MslI CAynn'nnrTG

Cuts at:      0     748    1369    2355 
   Size:        748     621     986

  Fragments arranged by size: 

                 986     748     621
 
MspA1I CmG'CkG

Cuts at:      0    1299    2355 
   Size:       1299    1056
 
NsiI A_TGCA'T

Cuts at:      0    1751    2355 
   Size:       1751     604
 
NspI r_CATG'y

Cuts at:      0     644    2355 
   Size:        644    1711
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     876    2355 
   Size:        876    1479
 
PfoI T'CCnGG_A

Cuts at:      0    1194    2355 
   Size:       1194    1161
 
PpuMI rG'GwC_Cy

Cuts at:      0    2123    2355 
   Size:       2123     232
 
PsiI TTA'TAA

Cuts at:      0    1582    1916    2345    2355 
   Size:       1582     334     429      10

  Fragments arranged by size: 

                1582     429     334      10
 
PstI C_TGCA'G

Cuts at:      0     189    2355 
   Size:        189    2166
 
PvuII CAG'CTG

Cuts at:      0    1299    2355 
   Size:       1299    1056
 
SanDI GG'GwC_CC

Cuts at:      0    2123    2355 
   Size:       2123     232
 
SfcI C'TryA_G

Cuts at:      0     185     586    2355 
   Size:        185     401    1769

  Fragments arranged by size: 

                1769     401     185
 
SmlI C'TyrA_G

Cuts at:      0     125    2355 
   Size:        125    2230
 
SnaBI TAC'GTA

Cuts at:      0    2205    2355 
   Size:       2205     150
 
SpeI A'CTAG_T

Cuts at:      0    2107    2355 
   Size:       2107     248
 
SspI AAT'ATT

Cuts at:      0     342    1510    2175    2355 
   Size:        342    1168     665     180

  Fragments arranged by size: 

                1168     665     342     180
 
StyI C'CwwG_G

Cuts at:      0     532     860    1092    2355 
   Size:        532     328     232    1263

  Fragments arranged by size: 

                1263     532     328     232
 
TatI w'GTAC_w

Cuts at:      0    1377    2074    2355 
   Size:       1377     697     281

  Fragments arranged by size: 

                1377     697     281
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1823    1855    2355 
   Size:       1823      32     500

  Fragments arranged by size: 

                1823     500      32
 
XmnI GAAnn'nnTTC

Cuts at:      0    1355    2355 
   Size:       1355    1000
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AlfI     ApoI     AseI     BbsI     BclI
    BfrBI     BmrI     BpmI    BpuEI    BsaAI    BsaXI    BseRI    BseYI
  BsiHKAI     BsmI Bsp1286I   BstAPI  BstZ17I     BtsI    CspCI     DraI
     EarI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI    Hin4I   HincII
  HindIII     MluI     MslI   MspA1I     NsiI     NspI    PflMI     PfoI
    PpuMI     PsiI     PstI    PvuII    SanDI     SfcI     SmlI    SnaBI
     SpeI     SspI     StyI     TatI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AloI    AlwNI     ApaI    ApaLI     AscI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI     BcgI
    BciVI     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI
   Bpu10I     BsaI    BsaBI    BsaHI    BsaWI     BsgI    BsiEI    BsiWI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     ClaI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI
    EcoNI     FseI     FspI    FspAI    HaeII     HpaI     KasI     KpnI
     MfeI     MmeI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     PacI     PasI     PciI     PmeI     PmlI
     PpiI    PshAI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SphI     SrfI     StuI     SwaI    TaqII
  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
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