Restriction map for
pF1KB8467
(Circular) (Six-base) MAPSORT of: pF1KB8467.seq Check: 5387 from: 1 to: 4460
pF1KB8467 4460 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 1139 1498 1139
Size: 359 4101
Acc65I G'GTAC_C
Cuts at: 1476 1476
Size: 4460
AclI AA'CG_TT
Cuts at: 4300 4300
Size: 4460
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2285 2717 3070 3732 2285
Size: 432 353 662 3013
Fragments arranged by size:
3013 662 432 353
AflIII A'CryG_T
Cuts at: 1812 3612 3893 1812
Size: 1800 281 2379
Fragments arranged by size:
2379 1800 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 179 211 179
Size: 32 4428
AlwNI CAG_nnn'CTG
Cuts at: 989 1765 3203 989
Size: 776 1438 2246
Fragments arranged by size:
2246 1438 776
ApaI G_GGCC'C
Cuts at: 849 1275 849
Size: 426 4034
ApaLI G'TGCA_C
Cuts at: 3298 3298
Size: 4460
ApoI r'AATT_y
Cuts at: 1357 1450 1464 3813 4069 1357
Size: 93 14 2349 256 1748
Fragments arranged by size:
2349 1748 256 93 14
AseI AT'TA_AT
Cuts at: 20 20
Size: 4460
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4460
AvaI C'yCGr_G
Cuts at: 296 419 1026 1198 1480 296
Size: 123 607 172 282 3276
Fragments arranged by size:
3276 607 282 172 123
BamHI G'GATC_C
Cuts at: 1485 1485
Size: 4460
BanI G'GyrC_C
Cuts at: 885 1077 1476 1890 2138 2173 885
Size: 192 399 414 248 35 3172
Fragments arranged by size:
3172 414 399 248 192 35
BanII G_rGCy'C
Cuts at: 176 659 849 1001 1275 1474 2504 176
Size: 483 190 152 274 199 1030 2132
Fragments arranged by size:
2132 1030 483 274 199 190 152
BbeI G_GCGC'C
Cuts at: 1894 2142 1894
Size: 248 4212
BbsI GAAGACnn'nnnn_
Cuts at: 1382 1382
Size: 4460
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4426
BciVI GTATCCnnnnn_n'
Cuts at: 2358 3414 4460 2358
Size: 1056 1046 2358
Fragments arranged by size:
2358 1056 1046
BclI T'GATC_A
Cuts at: 383 1980 383
Size: 1597 2863
BfrBI ATG'CAT
Cuts at: 119 119
Size: 4460
BglI GCCn_nnn'nGGC
Cuts at: 506 1312 506
Size: 806 3654
BglII A'GATC_T
Cuts at: 312 1975 4012 312
Size: 1663 2037 760
Fragments arranged by size:
2037 1663 760
BlpI GC'TnA_GC
Cuts at: 1240 1572 1240
Size: 332 4128
Bme1580I G_kGCm'C
Cuts at: 296 541 849 890 1082 1275 2085 2178 3302
Size: 245 308 41 192 193 810 93 1124
Cuts at: 3302 296
Size: 1454
Fragments arranged by size:
1454 1124 810 308 245 193 192 93
41
BmgBI CAC'GTC
Cuts at: 1074 1074
Size: 4460
BmrI ACTGGGnnnn_n'
Cuts at: 1672 1753 2088 4232 1672
Size: 81 335 2144 1900
Fragments arranged by size:
2144 1900 335 81
BmtI G_CTAG'C
Cuts at: 230 230
Size: 4460
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2827 2827
Size: 4460
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 628 1411 337
Size: 291 783 3386
Fragments arranged by size:
3386 783 291
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 206 1636 2515 3229 3527 206
Size: 1430 879 714 298 1139
Fragments arranged by size:
1430 1139 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 642 1199 4164 642
Size: 557 2965 938
Fragments arranged by size:
2965 938 557
BsaAI yAC'GTr
Cuts at: 132 1813 132
Size: 1681 2779
BsaBI GATnn'nnATC
Cuts at: 6 1974 4011 6
Size: 1968 2037 455
Fragments arranged by size:
2037 1968 455
BsaHI Gr'CG_yC
Cuts at: 1891 2139 2841 4337 1891
Size: 248 702 1496 2014
Fragments arranged by size:
2014 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1649 2170 2848 3259 3406 3680 1649
Size: 521 678 411 147 274 2429
Fragments arranged by size:
2429 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 167 624 1885 2643 3308 3716 167
Size: 457 1261 758 665 408 911
Fragments arranged by size:
1261 911 758 665 457 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1240 1240
Size: 4460
BsiEI CG_ry'CG
Cuts at: 104 1190 2002 2048 3278 104
Size: 1086 812 46 1230 1286
Fragments arranged by size:
1286 1230 1086 812 46
BsiHKAI G_wGCw'C
Cuts at: 176 1474 2252 2442 3302 176
Size: 1298 778 190 860 1334
Fragments arranged by size:
1334 1298 860 778 190
BsmI GAATG_Cn'
Cuts at: 121 121
Size: 4460
Bsp1286I G_dGCh'C
Cuts at: 176 296 541 659 849 890 1001 1082 1275
Size: 120 245 118 190 41 111 81 193
Cuts at: 1275 1474 2085 2178 2252 2442 2504 3302 176
Size: 199 611 93 74 190 62 798 1334
Fragments arranged by size:
1334 798 611 245 199 193 190 190
120 118 111 93 81 74 62 41
BspHI T'CATG_A
Cuts at: 305 2892 305
Size: 2587 1873
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1510 1724 2026 2407 1510
Size: 214 302 381 3563
Fragments arranged by size:
3563 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2752 2806 4290 4455 50
Size: 2702 54 1484 165 55
Fragments arranged by size:
2702 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 165 285 1256 2372 165
Size: 120 971 1116 2253
Fragments arranged by size:
2253 1116 971 120
BsrFI r'CCGG_y
Cuts at: 2458 2639 2458
Size: 181 4279
BssSI C'ACGA_G
Cuts at: 2731 3439 2731
Size: 708 3752
BstAPI GCAn_nnn'nTGC
Cuts at: 164 1837 164
Size: 1673 2787
BstBI TT'CG_AA
Cuts at: 2821 2821
Size: 4460
BstEII G'GTnAC_C
Cuts at: 915 915
Size: 4460
BstXI CCAn_nnnn'nTGG
Cuts at: 529 529
Size: 4460
BstYI r'GATC_y
Cuts at: 1 312 1485 1952 1975 2310 2556 2862 2874
Size: 311 1173 467 23 335 246 306 12
Cuts at: 2874 2960 4012 1
Size: 86 1052 449
Fragments arranged by size:
1173 1052 467 449 335 311 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 1190 1250 1190
Size: 60 4400
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2410 2591 2754 2410
Size: 181 163 4116
Fragments arranged by size:
4116 181 163
BtsI GCAGTG_nn'
Cuts at: 653 821 1703 653
Size: 168 882 3410
Fragments arranged by size:
3410 882 168
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1239 1274 1239
Size: 35 4425
DraI TTT'AAA
Cuts at: 1459 1459
Size: 4460
DrdI GACnn_nn'nnGTC
Cuts at: 2166 3510 2166
Size: 1344 3116
EaeI y'GGCC_r
Cuts at: 971 1211 1253 2045 2219 2610 2637 3982 971
Size: 240 42 792 174 391 27 1345 1449
Fragments arranged by size:
1449 1345 792 391 240 174 42 27
EagI C'GGCC_G
Cuts at: 2045 2045
Size: 4460
EarI CTCTTCn'nnn_
Cuts at: 181 370 2483 2693 181
Size: 189 2113 210 1948
Fragments arranged by size:
2113 1948 210 189
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2668 3410 3556 4270 2668
Size: 742 146 714 2858
Fragments arranged by size:
2858 742 714 146
EcoICRI GAG'CTC
Cuts at: 174 1472 174
Size: 1298 3162
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 628 1411 2285 2717 3070 3732 337
Size: 291 783 874 432 353 662 1065
Fragments arranged by size:
1065 874 783 662 432 353 291
EcoNI CCTnn'n_nnAGG
Cuts at: 1213 1393 1213
Size: 180 4280
EcoO109I rG'GnC_Cy
Cuts at: 845 846 1599 845
Size: 1 753 3706
Fragments arranged by size:
3706 753 1
EcoRI G'AATT_C
Cuts at: 1464 1464
Size: 4460
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1332 2479 1332
Size: 1147 3313
FspI TGC'GCA
Cuts at: 2241 2241
Size: 4460
HaeII r_GCGC'y
Cuts at: 771 1894 2142 3372 4143 771
Size: 1123 248 1230 771 1088
Fragments arranged by size:
1230 1123 1088 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 232 264 478 510 2302 2332 2334 2364 232
Size: 32 214 32 1792 30 2 30 2328
Fragments arranged by size:
2328 1792 214 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1499 1499
Size: 4460
KasI G'GCGC_C
Cuts at: 1890 2138 1890
Size: 248 4212
KpnI G_GTAC'C
Cuts at: 1480 1480
Size: 4460
MluI A'CGCG_T
Cuts at: 3893 3893
Size: 4460
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3219 3403 3219
Size: 184 4276
MscI TGG'CCA
Cuts at: 1213 2221 1213
Size: 1008 3452
MslI CAynn'nnrTG
Cuts at: 1328 2449 2576 1328
Size: 1121 127 3212
Fragments arranged by size:
3212 1121 127
MspA1I CmG'CkG
Cuts at: 381 912 1224 1571 1885 2245 3029 3274 381
Size: 531 312 347 314 360 784 245 1567
Fragments arranged by size:
1567 784 531 360 347 314 312 245
NaeI GCC'GGC
Cuts at: 2641 2641
Size: 4460
NarI GG'CG_CC
Cuts at: 1891 2139 1891
Size: 248 4212
NgoMIV G'CCGG_C
Cuts at: 2639 2639
Size: 4460
NheI G'CTAG_C
Cuts at: 226 226
Size: 4460
NsiI A_TGCA'T
Cuts at: 121 121
Size: 4460
NspI r_CATG'y
Cuts at: 292 1059 1239 1513 2013 3616 292
Size: 767 180 274 500 1603 1136
Fragments arranged by size:
1603 1136 767 500 274 180
PciI A'CATG_T
Cuts at: 3612 3612
Size: 4460
PmeI GTTT'AAAC
Cuts at: 1459 1459
Size: 4460
PmlI CAC'GTG
Cuts at: 132 132
Size: 4460
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2906 2938 2906
Size: 32 4428
PshAI GACnn'nnGTC
Cuts at: 1145 1192 1145
Size: 47 4413
PspOMI G'GGCC_C
Cuts at: 845 1271 845
Size: 426 4034
PstI C_TGCA'G
Cuts at: 506 648 1507 2192 506
Size: 142 859 685 2774
Fragments arranged by size:
2774 859 685 142
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4460
PvuII CAG'CTG
Cuts at: 381 1885 2245 381
Size: 1504 360 2596
Fragments arranged by size:
2596 1504 360
RsrII CG'GwC_CG
Cuts at: 2655 2655
Size: 4460
SacI G_AGCT'C
Cuts at: 176 1474 176
Size: 1298 3162
SalI G'TCGA_C
Cuts at: 1497 1497
Size: 4460
SapI GCTCTTCn'nnn_
Cuts at: 181 370 2483 2693 181
Size: 189 2113 210 1948
Fragments arranged by size:
2113 1948 210 189
SbfI CC_TGCA'GG
Cuts at: 506 1507 506
Size: 1001 3459
ScaI AGT'ACT
Cuts at: 3977 3977
Size: 4460
SexAI A'CCwGG_T
Cuts at: 455 881 455
Size: 426 4034
SfcI C'TryA_G
Cuts at: 33 326 502 644 1503 2188 3156 3347 33
Size: 293 176 142 859 685 968 191 1146
Fragments arranged by size:
1146 968 859 685 293 191 176 142
SfoI GGC'GCC
Cuts at: 1892 2140 1892
Size: 248 4212
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4460
SmaI CCC'GGG
Cuts at: 298 421 1200 1482 298
Size: 123 779 282 3276
Fragments arranged by size:
3276 779 282 123
SmlI C'TyrA_G
Cuts at: 221 1615 2530 3244 3506 221
Size: 1394 915 714 262 1175
Fragments arranged by size:
1394 1175 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4460
SphI G_CATG'C
Cuts at: 1059 1239 1513 2013 1059
Size: 180 274 500 3506
Fragments arranged by size:
3506 500 274 180
StuI AGG'CCT
Cuts at: 1267 1267
Size: 4460
StyI C'CwwG_G
Cuts at: 1082 1214 1594 1948 1082
Size: 132 380 354 3594
Fragments arranged by size:
3594 380 354 132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 694 2043 2176 2635 2844 694
Size: 1349 133 459 209 2310
Fragments arranged by size:
2310 1349 459 209 133
TatI w'GTAC_w
Cuts at: 1040 3975 1040
Size: 2935 1525
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3977 4009 3977
Size: 32 4428
Tth111I GACn'n_nGTC
Cuts at: 2257 2257
Size: 4460
XbaI T'CTAG_A
Cuts at: 1491 1491
Size: 4460
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 529 1008 1955 529
Size: 479 947 3034
Fragments arranged by size:
3034 947 479
XmaI C'CCGG_G
Cuts at: 296 419 1198 1480 296
Size: 123 779 282 3276
Fragments arranged by size:
3276 779 282 123
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NheI NsiI NspI PciI
PmeI PmlI PpiI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AscI AvrII BaeI BbvCI Bpu10I BsaXI BseRI BsiWI
BsmBI BspEI BsrGI BssHII BstZ17I Bsu36I ClaI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NotI NruI PacI PasI PflMI PfoI PpuMI PsiI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI