Restriction map for
pF1KB8466
(Circular) (Six-base) MAPSORT of: pF1KB8466.seq Check: 7407 from: 1 to: 4367
pF1KB8466 4367 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 685 685
Size: 4367
AccI GT'mk_AC
Cuts at: 257 350 1405 257
Size: 93 1055 3219
Fragments arranged by size:
3219 1055 93
Acc65I G'GTAC_C
Cuts at: 1383 1383
Size: 4367
AclI AA'CG_TT
Cuts at: 4207 4207
Size: 4367
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 320 2192 2624 2977 3639 320
Size: 1872 432 353 662 1048
Fragments arranged by size:
1872 1048 662 432 353
AflIII A'CryG_T
Cuts at: 197 1719 3519 3800 197
Size: 1522 1800 281 764
Fragments arranged by size:
1800 1522 764 281
AleI CACnn'nnGTG
Cuts at: 837 837
Size: 4367
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 855 1105 855
Size: 250 4117
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 409 441 409
Size: 32 4335
AlwNI CAG_nnn'CTG
Cuts at: 151 1672 3110 151
Size: 1521 1438 1408
Fragments arranged by size:
1521 1438 1408
ApaI G_GGCC'C
Cuts at: 211 1139 211
Size: 928 3439
ApaLI G'TGCA_C
Cuts at: 3205 3205
Size: 4367
ApoI r'AATT_y
Cuts at: 1371 3720 3976 1371
Size: 2349 256 1762
Fragments arranged by size:
2349 1762 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4367
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4367
AvaI C'yCGr_G
Cuts at: 651 694 964 1387 651
Size: 43 270 423 3631
Fragments arranged by size:
3631 423 270 43
BamHI G'GATC_C
Cuts at: 1392 1392
Size: 4367
BanI G'GyrC_C
Cuts at: 818 831 1249 1383 1797 2045 2080 818
Size: 13 418 134 414 248 35 3105
Fragments arranged by size:
3105 418 414 248 134 35 13
BanII G_rGCy'C
Cuts at: 211 383 989 1127 1139 1381 2411 211
Size: 172 606 138 12 242 1030 2167
Fragments arranged by size:
2167 1030 606 242 172 138 12
BbeI G_GCGC'C
Cuts at: 822 1801 2049 822
Size: 979 248 3140
Fragments arranged by size:
3140 979 248
BbsI GAAGACnn'nnnn_
Cuts at: 639 1153 639
Size: 514 3853
BbvCI CC'TCA_GC
Cuts at: 618 618
Size: 4367
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 955 989 955
Size: 34 4333
BciVI GTATCCnnnnn_n'
Cuts at: 2265 3321 4367 2265
Size: 1056 1046 2265
Fragments arranged by size:
2265 1056 1046
BclI T'GATC_A
Cuts at: 1887 1887
Size: 4367
BglI GCCn_nnn'nGGC
Cuts at: 166 895 166
Size: 729 3638
BglII A'GATC_T
Cuts at: 1882 3919 1882
Size: 2037 2330
BlpI GC'TnA_GC
Cuts at: 810 1479 810
Size: 669 3698
Bme1580I G_kGCm'C
Cuts at: 211 662 834 958 1139 1992 2085 3209 211
Size: 451 172 124 181 853 93 1124 1369
Fragments arranged by size:
1369 1124 853 451 181 172 124 93
BmrI ACTGGGnnnn_n'
Cuts at: 754 1334 1579 1660 1995 4139 754
Size: 580 245 81 335 2144 982
Fragments arranged by size:
2144 982 580 335 245 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 177 2734 177
Size: 2557 1810
Bpu10I CC'TnA_GC
Cuts at: 618 618
Size: 4367
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1543 2422 3136 3434 1543
Size: 879 714 298 2476
Fragments arranged by size:
2476 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4071 4071
Size: 4367
BsaAI yAC'GTr
Cuts at: 930 1720 930
Size: 790 3577
BsaBI GATnn'nnATC
Cuts at: 6 1348 1881 3918 6
Size: 1342 533 2037 455
Fragments arranged by size:
2037 1342 533 455
BsaHI Gr'CG_yC
Cuts at: 819 1798 2046 2748 4244 819
Size: 979 248 702 1496 942
Fragments arranged by size:
1496 979 942 702 248
BsaWI w'CCGG_w
Cuts at: 579 1556 2077 2755 3166 3313 3587 579
Size: 977 521 678 411 147 274 1359
Fragments arranged by size:
1359 977 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 153 982 1168 1171 1180 1183 1186 1198 153
Size: 829 186 3 9 3 3 12 3322
Fragments arranged by size:
3322 829 186 12 9 3 3 3
BseYI C'CCAG_C
Cuts at: 285 390 814 1273 1792 2550 3215 3623 285
Size: 105 424 459 519 758 665 408 1029
Fragments arranged by size:
1029 758 665 519 459 424 408 105
BsiEI CG_ry'CG
Cuts at: 104 740 1224 1270 1909 1955 3185 104
Size: 636 484 46 639 46 1230 1286
Fragments arranged by size:
1286 1230 639 636 484 46 46
BsiHKAI G_wGCw'C
Cuts at: 383 676 911 935 1381 2159 2349 3209 383
Size: 293 235 24 446 778 190 860 1541
Fragments arranged by size:
1541 860 778 446 293 235 190 24
BsmI GAATG_Cn'
Cuts at: 162 162
Size: 4367
Bsp1286I G_dGCh'C
Cuts at: 211 383 662 676 834 911 935 958 989
Size: 172 279 14 158 77 24 23 31
Cuts at: 989 1127 1139 1381 1992 2085 2159 2349 2411
Size: 138 12 242 611 93 74 190 62
Cuts at: 2411 3209 211
Size: 798 1369
Fragments arranged by size:
1369 798 611 279 242 190 172 158
138 93 77 74 62 31 24 23
14 12
BspHI T'CATG_A
Cuts at: 515 2799 515
Size: 2284 2083
BspMI ACCTGCnnnn'nnnn_
Cuts at: 685 794 1417 1631 1933 2314 685
Size: 109 623 214 302 381 2738
Fragments arranged by size:
2738 623 381 302 214 109
BsrBI CCG'CTC
Cuts at: 50 557 1266 2659 2713 4197 4362 50
Size: 507 709 1393 54 1484 165 55
Fragments arranged by size:
1484 1393 709 507 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2279 2279
Size: 4367
BsrFI r'CCGG_y
Cuts at: 699 718 2365 2546 699
Size: 19 1647 181 2520
Fragments arranged by size:
2520 1647 181 19
BssSI C'ACGA_G
Cuts at: 837 2638 3346 837
Size: 1801 708 1858
Fragments arranged by size:
1858 1801 708
BstAPI GCAn_nnn'nTGC
Cuts at: 503 1744 503
Size: 1241 3126
BstBI TT'CG_AA
Cuts at: 2728 2728
Size: 4367
BstXI CCAn_nnnn'nTGG
Cuts at: 1281 1281
Size: 4367
BstYI r'GATC_y
Cuts at: 1 1392 1859 1882 2217 2463 2769 2781 2867
Size: 1391 467 23 335 246 306 12 86
Cuts at: 2867 3919 1
Size: 1052 449
Fragments arranged by size:
1391 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 539 623 638 663 890 107
Size: 432 84 15 25 227 3584
Fragments arranged by size:
3584 432 227 84 25 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2317 2498 2661 2317
Size: 181 163 4023
Fragments arranged by size:
4023 181 163
BtsI GCAGTG_nn'
Cuts at: 694 958 1610 694
Size: 264 652 3451
Fragments arranged by size:
3451 652 264
DraI TTT'AAA
Cuts at: 1366 1366
Size: 4367
DrdI GACnn_nn'nnGTC
Cuts at: 2073 3417 2073
Size: 1344 3023
EaeI y'GGCC_r
Cuts at: 227 326 371 407 536 737 887 1221 1267
Size: 99 45 36 129 201 150 334 46
Cuts at: 1267 1283 1952 2126 2517 2544 3889 227
Size: 16 669 174 391 27 1345 705
Fragments arranged by size:
1345 705 669 391 334 201 174 150
129 99 46 45 36 27 16
EagI C'GGCC_G
Cuts at: 737 1221 1267 1952 737
Size: 484 46 685 3152
Fragments arranged by size:
3152 685 484 46
EarI CTCTTCn'nnn_
Cuts at: 388 907 2390 2600 388
Size: 519 1483 210 2155
Fragments arranged by size:
2155 1483 519 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2575 3317 3463 4177 2575
Size: 742 146 714 2765
Fragments arranged by size:
2765 742 714 146
EcoICRI GAG'CTC
Cuts at: 381 1379 381
Size: 998 3369
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 320 2192 2624 2977 3639 320
Size: 1872 432 353 662 1048
Fragments arranged by size:
1872 1048 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 1086 1135 1136 1506 1086
Size: 49 1 370 3947
Fragments arranged by size:
3947 370 49 1
EcoRI G'AATT_C
Cuts at: 1371 1371
Size: 4367
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1092 2386 1092
Size: 1294 3073
FspI TGC'GCA
Cuts at: 1114 1348 2148 1114
Size: 234 800 3333
Fragments arranged by size:
3333 800 234
FspAI rTGC'GCAy
Cuts at: 1348 1348
Size: 4367
HaeII r_GCGC'y
Cuts at: 370 822 858 901 1009 1801 2049 3279 4050
Size: 452 36 43 108 792 248 1230 771
Cuts at: 4050 370
Size: 687
Fragments arranged by size:
1230 792 771 687 452 248 108 43
36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2209 2239 2241 2271 2209
Size: 30 2 30 4305
Fragments arranged by size:
4305 30 30 2
HincII GTy'rAC
Cuts at: 945 1406 945
Size: 461 3906
KasI G'GCGC_C
Cuts at: 818 1797 2045 818
Size: 979 248 3140
Fragments arranged by size:
3140 979 248
KpnI G_GTAC'C
Cuts at: 1387 1387
Size: 4367
MluI A'CGCG_T
Cuts at: 3800 3800
Size: 4367
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3126 3310 3126
Size: 184 4183
MscI TGG'CCA
Cuts at: 1285 2128 1285
Size: 843 3524
MslI CAynn'nnrTG
Cuts at: 112 307 469 520 796 837 877 883 2356
Size: 195 162 51 276 41 40 6 1473
Cuts at: 2356 2483 112
Size: 127 1996
Fragments arranged by size:
1996 1473 276 195 162 127 51 41
40 6
MspA1I CmG'CkG
Cuts at: 566 665 762 814 846 1025 1256 1478 1792
Size: 99 97 52 32 179 231 222 314
Cuts at: 1792 2152 2936 3181 566
Size: 360 784 245 1752
Fragments arranged by size:
1752 784 360 314 245 231 222 179
99 97 52 32
NaeI GCC'GGC
Cuts at: 701 720 2548 701
Size: 19 1828 2520
Fragments arranged by size:
2520 1828 19
NarI GG'CG_CC
Cuts at: 819 1798 2046 819
Size: 979 248 3140
Fragments arranged by size:
3140 979 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4367
NgoMIV G'CCGG_C
Cuts at: 699 718 2546 699
Size: 19 1828 2520
Fragments arranged by size:
2520 1828 19
NotI GC'GGCC_GC
Cuts at: 1267 1267
Size: 4367
NspI r_CATG'y
Cuts at: 117 687 1420 1920 3523 117
Size: 570 733 500 1603 961
Fragments arranged by size:
1603 961 733 570 500
PasI CC'CwG_GG
Cuts at: 189 189
Size: 4367
PciI A'CATG_T
Cuts at: 3519 3519
Size: 4367
PmeI GTTT'AAAC
Cuts at: 1366 1366
Size: 4367
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2813 2845 2813
Size: 32 4335
PpuMI rG'GwC_Cy
Cuts at: 1086 1086
Size: 4367
PspOMI G'GGCC_C
Cuts at: 207 1135 207
Size: 928 3439
PstI C_TGCA'G
Cuts at: 1414 2099 1414
Size: 685 3682
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4367
PvuII CAG'CTG
Cuts at: 814 1025 1792 2152 814
Size: 211 767 360 3029
Fragments arranged by size:
3029 767 360 211
RsrII CG'GwC_CG
Cuts at: 2562 2562
Size: 4367
SacI G_AGCT'C
Cuts at: 383 1381 383
Size: 998 3369
SacII CC_GC'GG
Cuts at: 666 666
Size: 4367
SalI G'TCGA_C
Cuts at: 1404 1404
Size: 4367
SapI GCTCTTCn'nnn_
Cuts at: 388 907 2390 2600 388
Size: 519 1483 210 2155
Fragments arranged by size:
2155 1483 519 210
SbfI CC_TGCA'GG
Cuts at: 1414 1414
Size: 4367
ScaI AGT'ACT
Cuts at: 3884 3884
Size: 4367
SexAI A'CCwGG_T
Cuts at: 260 1358 260
Size: 1098 3269
SfcI C'TryA_G
Cuts at: 33 1410 2095 3063 3254 33
Size: 1377 685 968 191 1146
Fragments arranged by size:
1377 1146 968 685 191
SfoI GGC'GCC
Cuts at: 820 1799 2047 820
Size: 979 248 3140
Fragments arranged by size:
3140 979 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4367
SmaI CCC'GGG
Cuts at: 1389 1389
Size: 4367
SmlI C'TyrA_G
Cuts at: 1522 2437 3151 3413 1522
Size: 915 714 262 2476
Fragments arranged by size:
2476 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4367
SphI G_CATG'C
Cuts at: 1420 1920 1420
Size: 500 3867
StyI C'CwwG_G
Cuts at: 107 1501 1855 107
Size: 1394 354 2619
Fragments arranged by size:
2619 1394 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 600 1950 2083 2542 2751 600
Size: 1350 133 459 209 2216
Fragments arranged by size:
2216 1350 459 209 133
TatI w'GTAC_w
Cuts at: 3882 3882
Size: 4367
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3884 3916 3884
Size: 32 4335
Tth111I GACn'n_nGTC
Cuts at: 2164 2164
Size: 4367
XbaI T'CTAG_A
Cuts at: 1398 1398
Size: 4367
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1862 1862
Size: 4367
XmaI C'CCGG_G
Cuts at: 1387 1387
Size: 4367
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NotI NspI PasI PciI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AscI AvrII BaeI
BfrBI BmgBI BmtI BpmI BsaXI BsgI BsiWI BsmBI
BspEI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI HindIII HpaI MfeI NdeI
NheI NruI NsiI PacI PflMI PfoI PmlI PshAI
PsiI PspXI PsrI SanDI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI