(Circular) (Six-base) MAPSORT of: pF1KB8465.seq Check: 8171 from: 1 to: 3491 pF1KB8465 3491 bp With 182 enzymes: SgfI * June 20, 2008 10:29 .. AccI GT'mk_AC Cuts at: 290 529 290 Size: 239 3252 Acc65I G'GTAC_C Cuts at: 507 507 Size: 3491 AclI AA'CG_TT Cuts at: 3331 3331 Size: 3491 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 358 1316 1748 2101 2763 358 Size: 958 432 353 662 1086 Fragments arranged by size: 1086 958 662 432 353 AflIII A'CryG_T Cuts at: 163 843 2643 2924 163 Size: 680 1800 281 730 Fragments arranged by size: 1800 730 680 281 AgeI A'CCGG_T Cuts at: 178 178 Size: 3491 AlwNI CAG_nnn'CTG Cuts at: 391 796 2234 391 Size: 405 1438 1648 Fragments arranged by size: 1648 1438 405 ApaLI G'TGCA_C Cuts at: 2329 2329 Size: 3491 ApoI r'AATT_y Cuts at: 495 2844 3100 495 Size: 2349 256 886 Fragments arranged by size: 2349 886 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3491 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3491 AvaI C'yCGr_G Cuts at: 511 511 Size: 3491 BamHI G'GATC_C Cuts at: 516 516 Size: 3491 BanI G'GyrC_C Cuts at: 507 921 1169 1204 507 Size: 414 248 35 2794 Fragments arranged by size: 2794 414 248 35 BanII G_rGCy'C Cuts at: 505 1535 505 Size: 1030 2461 BbeI G_GCGC'C Cuts at: 925 1173 925 Size: 248 3243 BciVI GTATCCnnnnn_n' Cuts at: 1389 2445 3491 1389 Size: 1056 1046 1389 Fragments arranged by size: 1389 1056 1046 BclI T'GATC_A Cuts at: 1011 1011 Size: 3491 BglI GCCn_nnn'nGGC Cuts at: 427 427 Size: 3491 BglII A'GATC_T Cuts at: 1006 3043 1006 Size: 2037 1454 BlpI GC'TnA_GC Cuts at: 603 603 Size: 3491 Bme1580I G_kGCm'C Cuts at: 1116 1209 2333 1116 Size: 93 1124 2274 Fragments arranged by size: 2274 1124 93 BmgBI CAC'GTC Cuts at: 448 460 448 Size: 12 3479 BmrI ACTGGGnnnn_n' Cuts at: 703 784 1119 3263 703 Size: 81 335 2144 931 Fragments arranged by size: 2144 931 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1858 1858 Size: 3491 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 301 301 Size: 3491 Bpu10I CC'TnA_GC Cuts at: 384 384 Size: 3491 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 400 667 1546 2260 2558 400 Size: 267 879 714 298 1333 Fragments arranged by size: 1333 879 714 298 267 BsaI GGTCTCn'nnnn_ Cuts at: 3195 3195 Size: 3491 BsaAI yAC'GTr Cuts at: 844 844 Size: 3491 BsaBI GATnn'nnATC Cuts at: 6 1005 3042 6 Size: 999 2037 455 Fragments arranged by size: 2037 999 455 BsaHI Gr'CG_yC Cuts at: 922 1170 1872 3368 922 Size: 248 702 1496 1045 Fragments arranged by size: 1496 1045 702 248 BsaWI w'CCGG_w Cuts at: 178 680 1201 1879 2290 2437 2711 178 Size: 502 521 678 411 147 274 958 Fragments arranged by size: 958 678 521 502 411 274 147 BseYI C'CCAG_C Cuts at: 916 1674 2339 2747 916 Size: 758 665 408 1660 Fragments arranged by size: 1660 758 665 408 BsiEI CG_ry'CG Cuts at: 104 1033 1079 2309 104 Size: 929 46 1230 1286 Fragments arranged by size: 1286 1230 929 46 BsiHKAI G_wGCw'C Cuts at: 505 1283 1473 2333 505 Size: 778 190 860 1663 Fragments arranged by size: 1663 860 778 190 BsmBI CGTCTCn'nnnn_ Cuts at: 163 163 Size: 3491 Bsp1286I G_dGCh'C Cuts at: 505 1116 1209 1283 1473 1535 2333 505 Size: 611 93 74 190 62 798 1663 Fragments arranged by size: 1663 798 611 190 93 74 62 BspHI T'CATG_A Cuts at: 1923 1923 Size: 3491 BspMI ACCTGCnnnn'nnnn_ Cuts at: 276 541 755 1057 1438 276 Size: 265 214 302 381 2329 Fragments arranged by size: 2329 381 302 265 214 BsrBI CCG'CTC Cuts at: 50 1783 1837 3321 3486 50 Size: 1733 54 1484 165 55 Fragments arranged by size: 1733 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1403 1403 Size: 3491 BsrFI r'CCGG_y Cuts at: 178 1489 1670 178 Size: 1311 181 1999 Fragments arranged by size: 1999 1311 181 BssSI C'ACGA_G Cuts at: 1762 2470 1762 Size: 708 2783 BstAPI GCAn_nnn'nTGC Cuts at: 868 868 Size: 3491 BstBI TT'CG_AA Cuts at: 1852 1852 Size: 3491 BstYI r'GATC_y Cuts at: 1 516 983 1006 1341 1587 1893 1905 1991 Size: 515 467 23 335 246 306 12 86 Cuts at: 1991 3043 1 Size: 1052 449 Fragments arranged by size: 1052 515 467 449 335 306 246 86 23 12 BstZ17I GTA'TAC Cuts at: 291 291 Size: 3491 BtgI C'CryG_G Cuts at: 297 297 Size: 3491 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1441 1622 1785 1441 Size: 181 163 3147 Fragments arranged by size: 3147 181 163 BtsI GCAGTG_nn' Cuts at: 734 734 Size: 3491 DraI TTT'AAA Cuts at: 490 490 Size: 3491 DraIII CAC_nnn'GTG Cuts at: 159 159 Size: 3491 DrdI GACnn_nn'nnGTC Cuts at: 1197 2541 1197 Size: 1344 2147 EaeI y'GGCC_r Cuts at: 264 393 428 1076 1250 1641 1668 3013 264 Size: 129 35 648 174 391 27 1345 742 Fragments arranged by size: 1345 742 648 391 174 129 35 27 EagI C'GGCC_G Cuts at: 1076 1076 Size: 3491 EarI CTCTTCn'nnn_ Cuts at: 435 477 1514 1724 435 Size: 42 1037 210 2202 Fragments arranged by size: 2202 1037 210 42 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1699 2441 2587 3301 1699 Size: 742 146 714 1889 Fragments arranged by size: 1889 742 714 146 EcoICRI GAG'CTC Cuts at: 503 503 Size: 3491 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 301 358 1316 1748 2101 2763 301 Size: 57 958 432 353 662 1029 Fragments arranged by size: 1029 958 662 432 353 57 EcoO109I rG'GnC_Cy Cuts at: 630 630 Size: 3491 EcoRI G'AATT_C Cuts at: 495 495 Size: 3491 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1510 1510 Size: 3491 FspI TGC'GCA Cuts at: 1272 1272 Size: 3491 HaeII r_GCGC'y Cuts at: 925 1173 2403 3174 925 Size: 248 1230 771 1242 Fragments arranged by size: 1242 1230 771 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1333 1363 1365 1395 1333 Size: 30 2 30 3429 Fragments arranged by size: 3429 30 30 2 HincII GTy'rAC Cuts at: 530 530 Size: 3491 KasI G'GCGC_C Cuts at: 921 1169 921 Size: 248 3243 KpnI G_GTAC'C Cuts at: 511 511 Size: 3491 MluI A'CGCG_T Cuts at: 2924 2924 Size: 3491 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 263 2250 2434 263 Size: 1987 184 1320 Fragments arranged by size: 1987 1320 184 MscI TGG'CCA Cuts at: 1252 1252 Size: 3491 MslI CAynn'nnrTG Cuts at: 1480 1607 1480 Size: 127 3364 MspA1I CmG'CkG Cuts at: 279 602 916 1276 2060 2305 279 Size: 323 314 360 784 245 1465 Fragments arranged by size: 1465 784 360 323 314 245 NaeI GCC'GGC Cuts at: 1672 1672 Size: 3491 NarI GG'CG_CC Cuts at: 922 1170 922 Size: 248 3243 NgoMIV G'CCGG_C Cuts at: 1670 1670 Size: 3491 NspI r_CATG'y Cuts at: 167 336 544 1044 2647 167 Size: 169 208 500 1603 1011 Fragments arranged by size: 1603 1011 500 208 169 PciI A'CATG_T Cuts at: 163 2643 163 Size: 2480 1011 PmeI GTTT'AAAC Cuts at: 490 490 Size: 3491 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1937 1969 1937 Size: 32 3459 PstI C_TGCA'G Cuts at: 538 1223 538 Size: 685 2806 PvuI CG_AT'CG Cuts at: 104 104 Size: 3491 PvuII CAG'CTG Cuts at: 279 916 1276 279 Size: 637 360 2494 Fragments arranged by size: 2494 637 360 RsrII CG'GwC_CG Cuts at: 1686 1686 Size: 3491 SacI G_AGCT'C Cuts at: 505 505 Size: 3491 SalI G'TCGA_C Cuts at: 528 528 Size: 3491 SapI GCTCTTCn'nnn_ Cuts at: 1514 1724 1514 Size: 210 3281 SbfI CC_TGCA'GG Cuts at: 538 538 Size: 3491 ScaI AGT'ACT Cuts at: 134 3008 134 Size: 2874 617 SfcI C'TryA_G Cuts at: 33 534 1219 2187 2378 33 Size: 501 685 968 191 1146 Fragments arranged by size: 1146 968 685 501 191 SfiI GGCCn_nnn'nGGCC Cuts at: 427 427 Size: 3491 SfoI GGC'GCC Cuts at: 923 1171 923 Size: 248 3243 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3491 SgrAI Cr'CCGG_yG Cuts at: 178 178 Size: 3491 SmaI CCC'GGG Cuts at: 513 513 Size: 3491 SmlI C'TyrA_G Cuts at: 415 646 1561 2275 2537 415 Size: 231 915 714 262 1369 Fragments arranged by size: 1369 915 714 262 231 SpeI A'CTAG_T Cuts at: 67 67 Size: 3491 SphI G_CATG'C Cuts at: 544 1044 544 Size: 500 2991 StyI C'CwwG_G Cuts at: 378 625 979 378 Size: 247 354 2890 Fragments arranged by size: 2890 354 247 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 363 1074 1207 1666 1875 363 Size: 711 133 459 209 1979 Fragments arranged by size: 1979 711 459 209 133 TatI w'GTAC_w Cuts at: 132 3006 132 Size: 2874 617 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3008 3040 3008 Size: 32 3459 Tth111I GACn'n_nGTC Cuts at: 1288 1288 Size: 3491 XbaI T'CTAG_A Cuts at: 522 522 Size: 3491 XcmI CCAnnnn_n'nnnnTGG Cuts at: 986 986 Size: 3491 XmaI C'CCGG_G Cuts at: 511 511 Size: 3491 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BstZ17I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmtI BsaXI BseRI BsgI BsiWI BsmI BspEI BsrGI BssHII BstEII BstXI Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI