Restriction map for
pF1KB8463
(Circular) (Six-base) MAPSORT of: pF1KB8463.seq Check: 2726 from: 1 to: 4157
pF1KB8463 4157 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 573 573
Size: 4157
AatII G_ACGT'C
Cuts at: 803 803
Size: 4157
AccI GT'mk_AC
Cuts at: 1195 1195
Size: 4157
Acc65I G'GTAC_C
Cuts at: 1173 1173
Size: 4157
AclI AA'CG_TT
Cuts at: 3997 3997
Size: 4157
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 355 712 914 1982 2414 2767 3429 220
Size: 135 357 202 1068 432 353 662 948
Fragments arranged by size:
1068 948 662 432 357 353 202 135
AflIII A'CryG_T
Cuts at: 1509 3309 3590 1509
Size: 1800 281 2076
Fragments arranged by size:
2076 1800 281
AleI CACnn'nnGTG
Cuts at: 373 373
Size: 4157
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 668 697 1048 668
Size: 29 351 3777
Fragments arranged by size:
3777 351 29
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 589 621 589
Size: 32 4125
AlwNI CAG_nnn'CTG
Cuts at: 1462 2900 1462
Size: 1438 2719
ApaI G_GGCC'C
Cuts at: 189 534 189
Size: 345 3812
ApaLI G'TGCA_C
Cuts at: 2995 2995
Size: 4157
ApoI r'AATT_y
Cuts at: 1161 3510 3766 1161
Size: 2349 256 1552
Fragments arranged by size:
2349 1552 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4157
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4157
AvaI C'yCGr_G
Cuts at: 277 767 1015 1177 277
Size: 490 248 162 3257
Fragments arranged by size:
3257 490 248 162
BamHI G'GATC_C
Cuts at: 1182 1182
Size: 4157
BanI G'GyrC_C
Cuts at: 519 865 1173 1587 1835 1870 519
Size: 346 308 414 248 35 2806
Fragments arranged by size:
2806 414 346 308 248 35
BanII G_rGCy'C
Cuts at: 189 534 1171 2201 189
Size: 345 637 1030 2145
Fragments arranged by size:
2145 1030 637 345
BbeI G_GCGC'C
Cuts at: 1591 1839 1591
Size: 248 3909
BbvCI CC'TCA_GC
Cuts at: 706 706
Size: 4157
BciVI GTATCCnnnnn_n'
Cuts at: 2055 3111 4157 2055
Size: 1056 1046 2055
Fragments arranged by size:
2055 1056 1046
BclI T'GATC_A
Cuts at: 887 1677 887
Size: 790 3367
BfrBI ATG'CAT
Cuts at: 270 270
Size: 4157
BglI GCCn_nnn'nGGC
Cuts at: 250 661 916 988 250
Size: 411 255 72 3419
Fragments arranged by size:
3419 411 255 72
BglII A'GATC_T
Cuts at: 1672 3709 1672
Size: 2037 2120
BlpI GC'TnA_GC
Cuts at: 1269 1269
Size: 4157
Bme1580I G_kGCm'C
Cuts at: 189 534 870 1049 1782 1875 2999 189
Size: 345 336 179 733 93 1124 1347
Fragments arranged by size:
1347 1124 733 345 336 179 93
BmrI ACTGGGnnnn_n'
Cuts at: 466 829 1369 1450 1785 3929 466
Size: 363 540 81 335 2144 694
Fragments arranged by size:
2144 694 540 363 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2524 2524
Size: 4157
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 934 961 211
Size: 723 27 3407
Fragments arranged by size:
3407 723 27
Bpu10I CC'TnA_GC
Cuts at: 706 706
Size: 4157
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1073 1333 2212 2926 3224 1073
Size: 260 879 714 298 2006
Fragments arranged by size:
2006 879 714 298 260
BsaI GGTCTCn'nnnn_
Cuts at: 3861 3861
Size: 4157
BsaAI yAC'GTr
Cuts at: 1044 1510 1044
Size: 466 3691
BsaBI GATnn'nnATC
Cuts at: 6 1126 1671 3708 6
Size: 1120 545 2037 455
Fragments arranged by size:
2037 1120 545 455
BsaHI Gr'CG_yC
Cuts at: 800 1588 1836 2538 4034 800
Size: 788 248 702 1496 923
Fragments arranged by size:
1496 923 788 702 248
BsaWI w'CCGG_w
Cuts at: 1346 1867 2545 2956 3103 3377 1346
Size: 521 678 411 147 274 2126
Fragments arranged by size:
2126 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 199 229 589 619 199
Size: 30 360 30 3737
Fragments arranged by size:
3737 360 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 220 610 613 616 694 220
Size: 390 3 3 78 3683
Fragments arranged by size:
3683 390 78 3 3
BseYI C'CCAG_C
Cuts at: 466 1582 2340 3005 3413 466
Size: 1116 758 665 408 1210
Fragments arranged by size:
1210 1116 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 934 934
Size: 4157
BsiEI CG_ry'CG
Cuts at: 104 1699 1745 2975 104
Size: 1595 46 1230 1286
Fragments arranged by size:
1595 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 380 428 1171 1949 2139 2999 380
Size: 48 743 778 190 860 1538
Fragments arranged by size:
1538 860 778 743 190 48
BsmI GAATG_Cn'
Cuts at: 984 984
Size: 4157
Bsp1286I G_dGCh'C
Cuts at: 189 380 428 534 870 1049 1171 1782 1875
Size: 191 48 106 336 179 122 611 93
Cuts at: 1875 1949 2139 2201 2999 189
Size: 74 190 62 798 1347
Fragments arranged by size:
1347 798 611 336 191 190 179 122
106 93 74 62 48
BspHI T'CATG_A
Cuts at: 2589 2589
Size: 4157
BspMI ACCTGCnnnn'nnnn_
Cuts at: 573 1207 1421 1723 2104 573
Size: 634 214 302 381 2626
Fragments arranged by size:
2626 634 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2449 2503 3987 4152 50
Size: 2399 54 1484 165 55
Fragments arranged by size:
2399 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 681 2069 681
Size: 1388 2769
BsrFI r'CCGG_y
Cuts at: 862 2155 2336 862
Size: 1293 181 2683
Fragments arranged by size:
2683 1293 181
BsrGI T'GTAC_A
Cuts at: 491 491
Size: 4157
BssHII G'CGCG_C
Cuts at: 970 970
Size: 4157
BssSI C'ACGA_G
Cuts at: 2428 3136 2428
Size: 708 3449
BstAPI GCAn_nnn'nTGC
Cuts at: 1534 1534
Size: 4157
BstBI TT'CG_AA
Cuts at: 2518 2518
Size: 4157
BstXI CCAn_nnnn'nTGG
Cuts at: 1068 1068
Size: 4157
BstYI r'GATC_y
Cuts at: 1 1182 1649 1672 2007 2253 2559 2571 2657
Size: 1181 467 23 335 246 306 12 86
Cuts at: 2657 3709 1
Size: 1052 449
Fragments arranged by size:
1181 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 1061 107
Size: 954 3203
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2107 2288 2451 2107
Size: 181 163 3813
Fragments arranged by size:
3813 181 163
BtsI GCAGTG_nn'
Cuts at: 167 1400 167
Size: 1233 2924
DraI TTT'AAA
Cuts at: 1156 1156
Size: 4157
DrdI GACnn_nn'nnGTC
Cuts at: 631 1863 3207 631
Size: 1232 1344 1581
Fragments arranged by size:
1581 1344 1232
EaeI y'GGCC_r
Cuts at: 177 1094 1742 1916 2307 2334 3679 177
Size: 917 648 174 391 27 1345 655
Fragments arranged by size:
1345 917 655 648 391 174 27
EagI C'GGCC_G
Cuts at: 1742 1742
Size: 4157
EarI CTCTTCn'nnn_
Cuts at: 2180 2390 2180
Size: 210 3947
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2365 3107 3253 3967 2365
Size: 742 146 714 2555
Fragments arranged by size:
2555 742 714 146
EcoICRI GAG'CTC
Cuts at: 1169 1169
Size: 4157
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 220 355 712 914 934 961 1982 2414
Size: 9 135 357 202 20 27 1021 432
Cuts at: 2414 2767 3429 211
Size: 353 662 939
Fragments arranged by size:
1021 939 662 432 357 353 202 135
27 20 9
EcoNI CCTnn'n_nnAGG
Cuts at: 493 646 493
Size: 153 4004
EcoO109I rG'GnC_Cy
Cuts at: 186 222 530 663 990 1011 1029 1296 186
Size: 36 308 133 327 21 18 267 3047
Fragments arranged by size:
3047 327 308 267 133 36 21 18
EcoRI G'AATT_C
Cuts at: 1161 1161
Size: 4157
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2176 2176
Size: 4157
FspI TGC'GCA
Cuts at: 358 1938 358
Size: 1580 2577
FspAI rTGC'GCAy
Cuts at: 358 358
Size: 4157
HaeII r_GCGC'y
Cuts at: 713 1591 1839 3069 3840 713
Size: 878 248 1230 771 1030
Fragments arranged by size:
1230 1030 878 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 199 231 1999 2029 2031 2061 199
Size: 32 1768 30 2 30 2295
Fragments arranged by size:
2295 1768 32 30 30 2
HincII GTy'rAC
Cuts at: 1196 1196
Size: 4157
HindIII A'AGCT_T
Cuts at: 326 326
Size: 4157
KasI G'GCGC_C
Cuts at: 1587 1835 1587
Size: 248 3909
KpnI G_GTAC'C
Cuts at: 1177 1177
Size: 4157
MluI A'CGCG_T
Cuts at: 3590 3590
Size: 4157
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2916 3100 2916
Size: 184 3973
MscI TGG'CCA
Cuts at: 179 1918 179
Size: 1739 2418
MslI CAynn'nnrTG
Cuts at: 373 570 1066 2146 2273 373
Size: 197 496 1080 127 2257
Fragments arranged by size:
2257 1080 496 197 127
MspA1I CmG'CkG
Cuts at: 510 1110 1268 1582 1942 2726 2971 510
Size: 600 158 314 360 784 245 1696
Fragments arranged by size:
1696 784 600 360 314 245 158
NaeI GCC'GGC
Cuts at: 2338 2338
Size: 4157
NarI GG'CG_CC
Cuts at: 1588 1836 1588
Size: 248 3909
NcoI C'CATG_G
Cuts at: 107 1061 107
Size: 954 3203
NgoMIV G'CCGG_C
Cuts at: 2336 2336
Size: 4157
NsiI A_TGCA'T
Cuts at: 272 272
Size: 4157
NspI r_CATG'y
Cuts at: 507 847 1210 1710 3313 507
Size: 340 363 500 1603 1351
Fragments arranged by size:
1603 1351 500 363 340
PasI CC'CwG_GG
Cuts at: 666 666
Size: 4157
PciI A'CATG_T
Cuts at: 3309 3309
Size: 4157
PflMI CCAn_nnn'nTGG
Cuts at: 402 402
Size: 4157
PfoI T'CCnGG_A
Cuts at: 608 620 938 608
Size: 12 318 3827
Fragments arranged by size:
3827 318 12
PmeI GTTT'AAAC
Cuts at: 1156 1156
Size: 4157
PmlI CAC'GTG
Cuts at: 1044 1044
Size: 4157
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 589 621 2603 2635 589
Size: 32 1982 32 2111
Fragments arranged by size:
2111 1982 32 32
PpuMI rG'GwC_Cy
Cuts at: 222 222
Size: 4157
PspOMI G'GGCC_C
Cuts at: 185 530 185
Size: 345 3812
PstI C_TGCA'G
Cuts at: 215 584 647 1037 1204 1889 215
Size: 369 63 390 167 685 2483
Fragments arranged by size:
2483 685 390 369 167 63
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4157
PvuII CAG'CTG
Cuts at: 510 1582 1942 510
Size: 1072 360 2725
Fragments arranged by size:
2725 1072 360
RsrII CG'GwC_CG
Cuts at: 2352 2352
Size: 4157
SacI G_AGCT'C
Cuts at: 1171 1171
Size: 4157
SalI G'TCGA_C
Cuts at: 1194 1194
Size: 4157
SapI GCTCTTCn'nnn_
Cuts at: 2180 2390 2180
Size: 210 3947
SbfI CC_TGCA'GG
Cuts at: 647 1204 647
Size: 557 3600
ScaI AGT'ACT
Cuts at: 3674 3674
Size: 4157
SfcI C'TryA_G
Cuts at: 33 211 580 643 1033 1200 1885 2853 3044
Size: 178 369 63 390 167 685 968 191
Cuts at: 3044 33
Size: 1146
Fragments arranged by size:
1146 968 685 390 369 191 178 167
63
SfoI GGC'GCC
Cuts at: 1589 1837 1589
Size: 248 3909
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4157
SmaI CCC'GGG
Cuts at: 1179 1179
Size: 4157
SmlI C'TyrA_G
Cuts at: 767 1088 1312 2227 2941 3203 767
Size: 321 224 915 714 262 1721
Fragments arranged by size:
1721 915 714 321 262 224
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4157
SphI G_CATG'C
Cuts at: 1210 1710 1210
Size: 500 3657
StyI C'CwwG_G
Cuts at: 107 128 986 1061 1291 1645 107
Size: 21 858 75 230 354 2619
Fragments arranged by size:
2619 858 354 230 75 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 172 1740 1873 2332 2541 172
Size: 1568 133 459 209 1788
Fragments arranged by size:
1788 1568 459 209 133
TatI w'GTAC_w
Cuts at: 491 3672 491
Size: 3181 976
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3674 3706 3674
Size: 32 4125
Tth111I GACn'n_nGTC
Cuts at: 433 1954 433
Size: 1521 2636
XbaI T'CTAG_A
Cuts at: 420 1188 420
Size: 768 3389
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 663 1652 663
Size: 989 3168
XhoI C'TCGA_G
Cuts at: 767 767
Size: 4157
XmaI C'CCGG_G
Cuts at: 1177 1177
Size: 4157
ZraI GAC'GTC
Cuts at: 801 801
Size: 4157
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbvCI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AhdI AscI AvrII BaeI BbsI
BcgI BmgBI BmtI BsiWI BsmBI BspEI BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI HpaI MfeI
NdeI NheI NotI NruI PacI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XmnI