Restriction map for
pF1KB8463
(Linear) (Six-base) MAPSORT of: KIBB8463.seq Check: 9134 from: 1 to: 1044
KIBB8463.orf
With 182 enzymes: SgfI *
March 3, 2008 11:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 465 1044
Size: 465 579
AatII G_ACGT'C
Cuts at: 0 695 1044
Size: 695 349
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 247 604 806 1044
Size: 112 135 357 202 238
Fragments arranged by size:
357 238 202 135 112
AleI CACnn'nnGTG
Cuts at: 0 265 1044
Size: 265 779
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 560 589 940 1044
Size: 560 29 351 104
Fragments arranged by size:
560 351 104 29
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 481 513 1044
Size: 481 32 531
Fragments arranged by size:
531 481 32
ApaI G_GGCC'C
Cuts at: 0 81 426 1044
Size: 81 345 618
Fragments arranged by size:
618 345 81
AvaI C'yCGr_G
Cuts at: 0 169 659 907 1044
Size: 169 490 248 137
Fragments arranged by size:
490 248 169 137
BanI G'GyrC_C
Cuts at: 0 411 757 1044
Size: 411 346 287
Fragments arranged by size:
411 346 287
BanII G_rGCy'C
Cuts at: 0 81 426 1044
Size: 81 345 618
Fragments arranged by size:
618 345 81
BbvCI CC'TCA_GC
Cuts at: 0 598 1044
Size: 598 446
BclI T'GATC_A
Cuts at: 0 779 1044
Size: 779 265
BfrBI ATG'CAT
Cuts at: 0 162 1044
Size: 162 882
BglI GCCn_nnn'nGGC
Cuts at: 0 142 553 808 880 1044
Size: 142 411 255 72 164
Fragments arranged by size:
411 255 164 142 72
Bme1580I G_kGCm'C
Cuts at: 0 81 426 762 941 1044
Size: 81 345 336 179 103
Fragments arranged by size:
345 336 179 103 81
BmrI ACTGGGnnnn_n'
Cuts at: 0 358 721 1044
Size: 358 363 323
Fragments arranged by size:
363 358 323
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 826 853 1044
Size: 103 723 27 191
Fragments arranged by size:
723 191 103 27
Bpu10I CC'TnA_GC
Cuts at: 0 598 1044
Size: 598 446
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 965 1044
Size: 965 79
BsaAI yAC'GTr
Cuts at: 0 936 1044
Size: 936 108
BsaBI GATnn'nnATC
Cuts at: 0 1018 1044
Size: 1018 26
BsaHI Gr'CG_yC
Cuts at: 0 692 1044
Size: 692 352
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 91 121 481 511 1044
Size: 91 30 360 30 533
Fragments arranged by size:
533 360 91 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 112 502 505 508 586 1044
Size: 112 390 3 3 78 458
Fragments arranged by size:
458 390 112 78 3 3
BseYI C'CCAG_C
Cuts at: 0 358 1044
Size: 358 686
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 826 1044
Size: 826 218
BsiHKAI G_wGCw'C
Cuts at: 0 272 320 1044
Size: 272 48 724
Fragments arranged by size:
724 272 48
BsmI GAATG_Cn'
Cuts at: 0 876 1044
Size: 876 168
Bsp1286I G_dGCh'C
Cuts at: 0 81 272 320 426 762 941 1044
Size: 81 191 48 106 336 179 103
Fragments arranged by size:
336 191 179 106 103 81 48
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 465 1044
Size: 465 579
BsrDI GCAATG_nn'
Cuts at: 0 573 1044
Size: 573 471
BsrFI r'CCGG_y
Cuts at: 0 754 1044
Size: 754 290
BsrGI T'GTAC_A
Cuts at: 0 383 1044
Size: 383 661
BssHII G'CGCG_C
Cuts at: 0 862 1044
Size: 862 182
BstXI CCAn_nnnn'nTGG
Cuts at: 0 960 1044
Size: 960 84
BtgI C'CryG_G
Cuts at: 0 953 1044
Size: 953 91
BtsI GCAGTG_nn'
Cuts at: 0 59 1044
Size: 59 985
DrdI GACnn_nn'nnGTC
Cuts at: 0 523 1044
Size: 523 521
EaeI y'GGCC_r
Cuts at: 0 69 986 1044
Size: 69 917 58
Fragments arranged by size:
917 69 58
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 112 247 604 806 826 853 1044
Size: 103 9 135 357 202 20 27 191
Fragments arranged by size:
357 202 191 135 103 27 20 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 385 538 1044
Size: 385 153 506
Fragments arranged by size:
506 385 153
EcoO109I rG'GnC_Cy
Cuts at: 0 78 114 422 555 882 903 921 1044
Size: 78 36 308 133 327 21 18 123
Fragments arranged by size:
327 308 133 123 78 36 21 18
FspI TGC'GCA
Cuts at: 0 250 1044
Size: 250 794
FspAI rTGC'GCAy
Cuts at: 0 250 1044
Size: 250 794
HaeII r_GCGC'y
Cuts at: 0 605 1044
Size: 605 439
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 91 123 1044
Size: 91 32 921
Fragments arranged by size:
921 91 32
HindIII A'AGCT_T
Cuts at: 0 218 1044
Size: 218 826
MscI TGG'CCA
Cuts at: 0 71 1044
Size: 71 973
MslI CAynn'nnrTG
Cuts at: 0 265 462 958 1044
Size: 265 197 496 86
Fragments arranged by size:
496 265 197 86
MspA1I CmG'CkG
Cuts at: 0 402 1002 1044
Size: 402 600 42
Fragments arranged by size:
600 402 42
NcoI C'CATG_G
Cuts at: 0 953 1044
Size: 953 91
NsiI A_TGCA'T
Cuts at: 0 164 1044
Size: 164 880
NspI r_CATG'y
Cuts at: 0 399 739 1044
Size: 399 340 305
Fragments arranged by size:
399 340 305
PasI CC'CwG_GG
Cuts at: 0 558 1044
Size: 558 486
PflMI CCAn_nnn'nTGG
Cuts at: 0 294 1044
Size: 294 750
PfoI T'CCnGG_A
Cuts at: 0 500 512 830 1044
Size: 500 12 318 214
Fragments arranged by size:
500 318 214 12
PmlI CAC'GTG
Cuts at: 0 936 1044
Size: 936 108
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 481 513 1044
Size: 481 32 531
Fragments arranged by size:
531 481 32
PpuMI rG'GwC_Cy
Cuts at: 0 114 1044
Size: 114 930
PspOMI G'GGCC_C
Cuts at: 0 77 422 1044
Size: 77 345 622
Fragments arranged by size:
622 345 77
PstI C_TGCA'G
Cuts at: 0 107 476 539 929 1044
Size: 107 369 63 390 115
Fragments arranged by size:
390 369 115 107 63
PvuII CAG'CTG
Cuts at: 0 402 1044
Size: 402 642
SbfI CC_TGCA'GG
Cuts at: 0 539 1044
Size: 539 505
SfcI C'TryA_G
Cuts at: 0 103 472 535 925 1044
Size: 103 369 63 390 119
Fragments arranged by size:
390 369 119 103 63
SmlI C'TyrA_G
Cuts at: 0 659 980 1044
Size: 659 321 64
Fragments arranged by size:
659 321 64
StyI C'CwwG_G
Cuts at: 0 20 878 953 1044
Size: 20 858 75 91
Fragments arranged by size:
858 91 75 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 64 1044
Size: 64 980
TatI w'GTAC_w
Cuts at: 0 383 1044
Size: 383 661
Tth111I GACn'n_nGTC
Cuts at: 0 325 1044
Size: 325 719
XbaI T'CTAG_A
Cuts at: 0 312 1044
Size: 312 732
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 555 1044
Size: 555 489
XhoI C'TCGA_G
Cuts at: 0 659 1044
Size: 659 385
ZraI GAC'GTC
Cuts at: 0 693 1044
Size: 693 351
Enzymes that do cut:
AarI AatII AcuI AleI AlfI AloI ApaI AvaI
BanI BanII BbvCI BclI BfrBI BglI Bme1580I BmrI
BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI
BsrGI BssHII BstXI BtgI BtsI DrdI EaeI Eco57MI
EcoNI EcoO109I FspI FspAI HaeII Hin4I HindIII MscI
MslI MspA1I NcoI NsiI NspI PasI PflMI PfoI
PmlI PpiI PpuMI PspOMI PstI PvuII SbfI SfcI
SmlI StyI TaqII TatI Tth111I XbaI XcmI XhoI
ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbsI BcgI BciVI BglII BlpI BmgBI
BmtI BplI BsaI BsaWI BsiEI BsiWI BsmBI BspEI
BspHI BsrBI BssSI BstAPI BstBI BstEII BstYI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII EagI EarI
EciI EcoICRI EcoRI EcoRV FalI FseI HincII HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NdeI
NgoMIV NheI NotI NruI PacI PciI PmeI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TstI XmaI XmnI