Restriction map for pF1KB8460
 (Linear) (Six-base) MAPSORT of: KIBB8460.seq  Check: 7964  from: 1  to: 756



KIBB8460.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:06  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     609     756 
   Size:        609     147
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     598     756 
   Size:        598     158
 
AlwNI CAG_nnn'CTG

Cuts at:      0     468     681     756 
   Size:        468     213      75

  Fragments arranged by size: 

                 468     213      75
 
AvaI C'yCGr_G

Cuts at:      0     232     430     756 
   Size:        232     198     326

  Fragments arranged by size: 

                 326     232     198
 
BanII G_rGCy'C

Cuts at:      0     437     494     646     756 
   Size:        437      57     152     110

  Fragments arranged by size: 

                 437     152     110      57
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     615     756 
   Size:        615     141
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     193     756 
   Size:        193     563
 
BglII A'GATC_T

Cuts at:      0     138     756 
   Size:        138     618
 
BlpI GC'TnA_GC

Cuts at:      0     488     756 
   Size:        488     268
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     239     316     756 
   Size:        239      77     440

  Fragments arranged by size: 

                 440     239      77
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     109     586     696     756 
   Size:        109     477     110      60

  Fragments arranged by size: 

                 477     110     109      60
 
Bpu10I CC'TnA_GC

Cuts at:      0     436     756 
   Size:        436     320
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     247     756 
   Size:        247     509
 
BsaAI yAC'GTr

Cuts at:      0     181     756 
   Size:        181     575
 
BsaWI w'CCGG_w

Cuts at:      0     650     756 
   Size:        650     106
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     265     756 
   Size:        265     491
 
BseYI C'CCAG_C

Cuts at:      0     267     461     756 
   Size:        267     194     295

  Fragments arranged by size: 

                 295     267     194
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     304     756 
   Size:        304     452
 
BsiHKAI G_wGCw'C

Cuts at:      0     113     218     756 
   Size:        113     105     538

  Fragments arranged by size: 

                 538     113     105
 
Bsp1286I G_dGCh'C

Cuts at:      0     113     218     437     494     646     756 
   Size:        113     105     219      57     152     110

  Fragments arranged by size: 

                 219     152     113     110     105      57
 
BspHI T'CATG_A

Cuts at:      0     731     756 
   Size:        731      25
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     566     756 
   Size:        566     190
 
BstYI r'GATC_y

Cuts at:      0     138     756 
   Size:        138     618
 
Bsu36I CC'TnA_GG

Cuts at:      0     259     682     756 
   Size:        259     423      74

  Fragments arranged by size: 

                 423     259      74
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     580     756 
   Size:        580     176
 
BtsI GCAGTG_nn'

Cuts at:      0     216     756 
   Size:        216     540
 
DraIII CAC_nnn'GTG

Cuts at:      0     612     756 
   Size:        612     144
 
EaeI y'GGCC_r

Cuts at:      0     117     329     504     756 
   Size:        117     212     175     252

  Fragments arranged by size: 

                 252     212     175     117
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     109     586     609     696     756 
   Size:        109     477      23      87      60

  Fragments arranged by size: 

                 477     109      87      60      23
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     352     685     756 
   Size:        352     333      71

  Fragments arranged by size: 

                 352     333      71
 
EcoO109I rG'GnC_Cy

Cuts at:      0     236     512     678     756 
   Size:        236     276     166      78

  Fragments arranged by size: 

                 276     236     166      78
 
FspI TGC'GCA

Cuts at:      0     697     756 
   Size:        697      59
 
HaeII r_GCGC'y

Cuts at:      0     231     348     756 
   Size:        231     117     408

  Fragments arranged by size: 

                 408     231     117
 
MscI TGG'CCA

Cuts at:      0     119     756 
   Size:        119     637
 
MspA1I CmG'CkG

Cuts at:      0      89     465     747     756 
   Size:         89     376     282       9

  Fragments arranged by size: 

                 376     282      89       9
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     192     366     468     756 
   Size:        192     174     102     288

  Fragments arranged by size: 

                 288     192     174     102
 
PfoI T'CCnGG_A

Cuts at:      0     164     712     756 
   Size:        164     548      44

  Fragments arranged by size: 

                 548     164      44
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     433     465     756 
   Size:        433      32     291

  Fragments arranged by size: 

                 433     291      32
 
PstI C_TGCA'G

Cuts at:      0     223     290     410     577     756 
   Size:        223      67     120     167     179

  Fragments arranged by size: 

                 223     179     167     120      67
 
PvuII CAG'CTG

Cuts at:      0      89     465     747     756 
   Size:         89     376     282       9

  Fragments arranged by size: 

                 376     282      89       9
 
SfcI C'TryA_G

Cuts at:      0      80     219     286     406     573     756 
   Size:         80     139      67     120     167     183

  Fragments arranged by size: 

                 183     167     139     120      80      67
 
SmaI CCC'GGG

Cuts at:      0     234     756 
   Size:        234     522
 
SnaBI TAC'GTA

Cuts at:      0     181     756 
   Size:        181     575
 
StuI AGG'CCT

Cuts at:      0     191     756 
   Size:        191     565
 
StyI C'CwwG_G

Cuts at:      0     114     186     353     756 
   Size:        114      72     167     403

  Fragments arranged by size: 

                 403     167     114      72
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     162     756 
   Size:        162     594
 
XmaI C'CCGG_G

Cuts at:      0     232     756 
   Size:        232     524
 

 Enzymes that do cut:

     AcuI     AlfI    AlwNI     AvaI    BanII     BbsI    BciVI    BglII
     BlpI     BmrI     BpmI   Bpu10I     BsaI    BsaAI    BsaWI    BseRI
    BseYI     BsgI  BsiHKAI Bsp1286I    BspHI    BspMI    BstYI   Bsu36I
    BtgZI     BtsI   DraIII     EaeI  Eco57MI    EcoNI EcoO109I     FspI
    HaeII     MscI   MspA1I    PflMI     PfoI     PsrI     PstI    PvuII
     SfcI     SmaI    SnaBI     StuI     StyI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI
     BcgI     BclI    BfrBI     BglI Bme1580I    BmgBI     BmtI     BplI
    BpuEI    BsaBI    BsaHI    BsaXI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I     BtgI     ClaI    CspCI     DraI
     DrdI     EagI     EarI     EciI  EcoICRI    EcoRI    EcoRV     FalI
     FseI    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI
     SpeI     SphI     SrfI     SspI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XhoI     XmnI     ZraI
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