Restriction map for pF1KB8459
##################################
# NO CUT ENZYMES (  148 enzymes) #
##################################

AatII       AccI        AccIII      AcyI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BamHI       
BbeI        BbvCI       BciVI       BclI        BcnI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BsaHI       BsaI        BsaWI       BseAI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmBI       
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstXI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DdeI        DraII       DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoO109I    EcoO65I     EcoRV       EcoT22I     FbaI        
FseI        FspI        HapII       Hin1I       HpaI        
HpaII       KasI        KspI        MluI        MroI        
MslI        MspI        NaeI        NarI        NciI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmeI        PpuMI       
PshAI       PshBI       PstI        PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       StuI        
SwaI        Tth111I     Van91I      XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

Acc65I        195  GGTACC               G^GTACC
AccII        1250  CGCG                 CG^CG
Asp718I       195  GGTACC               G^GTACC
AvaII         777  GGACC                G^G[AT]CC
BanI          195  GGTACC               G^G[CT][GA]CC
BanII         846  GGGCTC               G[GA]GC[CT]^C
BbrPI         520  CACGTG               CAC^GTG
BbsI         1167  GAAGAC               GAAGAC(2/6)
BcgI         1305  CGAGAAGAATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          790  CTAG                 C^TAG
BpuAI        1167  GAAGAC               GAAGAC(2/6)
BsaAI         520  CACGTG               [CT]AC^GT[GA]
BsaBI         146  GATTTGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BssKI         889  CCTGG                ^CC[ACGT]GG
BstNI         889  CCTGG                CC^[AT]GG
BstUI        1250  CGCG                 CG^CG
BstYI         321  AGATCC               [GA]^GATC[CT]
Cac8I        1185  GCGGGC               GC[ACGT]^[ACGT]GC
DsaI           83  CCATGG               C^C[GA][CT]GG
EcoRI         618  GAATTC               G^AATTC
EcoRII        889  CCTGG                ^CC[AT]GG
HaeII         235  GGCGCT               [GA]GCGC^[CT]
HgaI          212  GACGC                GACGC(5/10)
HincII        699  GTCAAC               GT[CT]^[GA]AC
HindII        699  GTCAAC               GT[CT]^[GA]AC
HindIII       446  AAGCTT               A^AGCTT
KpnI          195  GGTACC               GGTAC^C
MaeI          790  CTAG                 C^TAG
MamI          146  GATTTGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1298  CAATTG               C^AATTG
MflI          321  AGATCC               [GA]^GATC[CT]
MunI         1298  CAATTG               C^AATTG
MvaI          889  CCTGG                CC^[AT]GG
MvnI         1250  CGCG                 CG^CG
NcoI           83  CCATGG               C^CATGG
PleI          697  GAGTC                GAGTC(4/5)
PmaCI         520  CACGTG               CAC^GTG
PmlI          520  CACGTG               CAC^GTG
PvuII         647  CAGCTG               CAG^CTG
ScrFI         889  CCTGG                CC^[ACGT]GG
SfcI         1154  CTACAG               C^T[GA][CT]AG
SspI          575  AATATT               AAT^ATT
TaqI          265  TCGA                 T^CGA
TthHB8I       265  TCGA                 T^CGA
XcmI          953  CCACTAAACCATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         321  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AcsI          618  GAATTC               [GA]^AATT[CT]
             1379  AAATTT              
AlwI          322  GATCC                GGATC(4/5)
               45  GGATC               
ApoI          618  GAATTC               [GA]^AATT[CT]
             1379  AAATTT              
BalI            2  TGGCCA               TGG^CCA
               86  TGGCCA              
BmyI          287  GTGCCC               G[AGT]GC[ACT]^C
              846  GGGCTC              
BsmAI         660  GTCTC                GTCTC(1/5)
              937  GTCTC               
BsmFI         404  GGGAC                GGGAC(10/14)
              862  GTCCC               
BsmI          994  GAATGC               GAATGC(1/-1)
             1311  GAATGC              
Bsp1286I      287  GTGCCC               G[AGT]GC[ACT]^C
              846  GGGCTC              
BstF5I       1359  GGATG                GGATG(2/0)
             1398  GGATG               
CfoI          132  GCGC                 GCG^C
              236  GCGC                
EcoNI         199  CCTTCTGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              250  CCTTTGGAAGG         
FauI          501  GCGGG                CCCGC(4/6)
             1185  GCGGG               
FokI         1359  GGATG                GGATG(9/13)
             1398  GGATG               
HhaI          132  GCGC                 GCG^C
              236  GCGC                
HinP1I        132  GCGC                 G^CGC
              236  GCGC                
MluNI           2  TGGCCA               TGG^CCA
               86  TGGCCA              
MscI            2  TGGCCA               TGG^CCA
               86  TGGCCA              
MspA1I        314  CAGCGG               C[AC]G^C[GT]G
              647  CAGCTG              
Tsp45I        983  GTGAC                ^GT[GC]AC
             1327  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   44 enzymes) #
#########################################

AciI        AclI        AfaI        AluI        BbvI        
BpmI        BsaJI       BseRI       BsiYI       BslI        
BsrI        Cfr13I      DpnII       DraI        EaeI        
EarI        EcoT14I     Fnu4HI      HaeIII      HinfI       
HphI        ItaI        Ksp632I     MaeII       MaeIII      
MboI        MboII       MnlI        MseI        MwoI        
NdeII       NlaIII      NlaIV       Psp1406I    RsaI        
Sau3AI      Sau96I      SfaNI       StyI        TfiI        
Tru9I       TseI        Tsp509I     TspRI       
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